24 research outputs found
Three-dimensional flow visualization system by holographic interferometry
A holographic system has been developed to visualize a three-dimensional fluid flow. The system consists principally of a thermoplastic film, a monochrome video camera, a microcomputer with image-processing capability and a printer. The system makes it possible to measure a slow flow. Two examples of such, the bimodal mixed-mode convection flows within a Bénard cell, are presented
Self-Contained Induction of Neurons from Human Embryonic Stem Cells
BACKGROUND: Neurons and glial cells can be efficiently induced from mouse embryonic stem (ES) cells in a conditioned medium collected from rat primary-cultured astrocytes (P-ACM). However, the use of rodent primary cells for clinical applications may be hampered by limited supply and risk of contamination with xeno-proteins. METHODOLOGY/PRINCIPAL FINDINGS: We have developed an alternative method for unimpeded production of human neurons under xeno-free conditions. Initially, neural stem cells in sphere-like clusters were induced from human ES (hES) cells after being cultured in P-ACM under free-floating conditions. The resultant neural stem cells could circumferentially proliferate under subsequent adhesive culture, and selectively differentiate into neurons or astrocytes by changing the medium to P-ACM or G5, respectively. These hES cell-derived neurons and astrocytes could procure functions similar to those of primary cells. Interestingly, a conditioned medium obtained from the hES cell-derived astrocytes (ES-ACM) could successfully be used to substitute P-ACM for induction of neurons. Neurons made by this method could survive in mice brain after xeno-transplantation. CONCLUSION/SIGNIFICANCE: By inducing astrocytes from hES cells in a chemically defined medium, we could produce human neurons without the use of P-ACM. This self-serving method provides an unlimited source of human neural cells and may facilitate clinical applications of hES cells for neurological diseases
A new technique for the visualization of ultrasonic traveling wave by linearized subfringe interferometric holography
Monolithically Integrated Waveband Selective Switch Using Cyclic AWGs
We propose a novel waveband-selective switch that uses cyclic Arrayed-Waveguide Gratings. The device is fabricated on one chip using Planer Lightwave Circuit technologies. Tests demonstrate that the designed performance is successfully realized