Segregation of molecules at cell division reveals native protein localization

We introduce a non-intrusive method exploiting post-division single-cell variability to validate protein localization. The results show that Clp proteases, widely reported to form biologically relevant foci, are in fact uniformly distributed inside Escherichia coli cells, and that many commonly used fluorescent proteins (FPs) cause severe mislocalization when fused to homo-oligomers. Re-tagging five other reportedly foci-forming proteins with the most monomeric FP tested suggests the foci were caused by the FPs

Revisiting the link between business strategy and performance: Evidence from hotels

This paper aims to investigate the competitive success factors for hotel companies and examine the link between business strategy and performance. Using a structured questionnaire, the researchers collected data from hotel managers in Spain. Study results suggest that a firm’s assets and strategies have a greater influence on performance than industry forces do. This lack of direct influence by industry forces is due to the sector’s specific characteristics, which cannot be overlooked during analysis. Based on these research findings, theoretical and managerial implications and future research are presented

Quantification of very low-abundant proteins in bacteria using the HaloTag and epi-fluorescence microscopy.

Cell biology is increasingly dependent on quantitative methods resulting in the need for microscopic labelling technologies that are highly sensitive and specific. Whilst the use of fluorescent proteins has led to major advances, they also suffer from their relatively low brightness and photo-stability, making the detection of very low abundance proteins using fluorescent protein-based methods challenging. Here, we characterize the use of the self-labelling protein tag called HaloTag, in conjunction with an organic fluorescent dye, to label and accurately count endogenous proteins present in very low numbers (<7) in individual Escherichia coli cells. This procedure can be used to detect single molecules in fixed cells with conventional epifluorescence illumination and a standard microscope. We show that the detection efficiency of proteins labelled with the HaloTag is ≥80%, which is on par or better than previous techniques. Therefore, this method offers a simple and attractive alternative to current procedures to detect low abundance molecules

Towards a circular maritime industry : identifying strategy and technology solutions

Shipping is considered one of the most energy-efficient modes, considering the amount of cargo that can be carried. On the other hand, the circular economy approach is not well-established in the maritime industry, which currently lags behind different transport modes. The maritime industry needs scientific support to “close the loop”, minimise waste and increase the revenue stream. Therefore, this study aims to address a critical gap in the maritime industry by first showing the understanding of the stakeholders and identifying suitable strategy and technology solutions that can fit the characteristics of the maritime industry. Moreover, the potential benefits of these solutions have been demonstrated through high-speed marine engine remanufacturing. A cost-benefit analysis has shown that remanufactured engine acquisition cost is nearly half of the cost of a new engine with similar operating performance and operating cost. This study is a novel contribution to maritime industry stakeholders to demonstrate the advantages of circular end-of-life applications

An approach to advance circular practices in the maritime industry through a database as a bridging solution

The concept of maritime circularity has gained increasing attention to address challenges arising from the net-zero targets of the maritime industry. The circular economy provides potential solutions to address these challenges through reuse, remanufacturing, and recycling practices. However, the industry faces complex challenges, including inefficient reverse supply chains, a lack of awareness about circular economy principles, standardisation issues, and the need for digital infrastructure to provide vital information in the sector. These challenges prevent the implementation of circularity practices, as access to crucial data throughout the vessel’s life cycle is obstructed. This novel research aims to create a robust first-of-its-kind database solution specifically designed to support the industry’s shift towards circularity. The database will facilitate fast and transparent information flow between the stakeholders, providing foundations for asset tracking and a robust reverse supply chain. A case study was conducted to show that a database could help extract higher financial value from end-of-life ships by over 80%. The ageing fleet increases the urgency of utilising such a database, which could be a pivotal strategy for a sustainable and circular industry. This digital solution offers significant benefits to all industry stakeholders and allows holistic resource management, influencing maritime operations’ sustainability, resilience, and profitability

“So what if ChatGPT wrote it?” Multidisciplinary perspectives on opportunities, challenges and implications of generative conversational AI for research, practice and policy

Transformative artificially intelligent tools, such as ChatGPT, designed to generate sophisticated text indistinguishable from that produced by a human, are applicable across a wide range of contexts. The technology presents opportunities as well as, often ethical and legal, challenges, and has the potential for both positive and negative impacts for organisations, society, and individuals. Offering multi-disciplinary insight into some of these, this article brings together 43 contributions from experts in fields such as computer science, marketing, information systems, education, policy, hospitality and tourism, management, publishing, and nursing. The contributors acknowledge ChatGPT's capabilities to enhance productivity and suggest that it is likely to offer significant gains in the banking, hospitality and tourism, and information technology industries, and enhance business activities, such as management and marketing. Nevertheless, they also consider its limitations, disruptions to practices, threats to privacy and security, and consequences of biases, misuse, and misinformation. However, opinion is split on whether ChatGPT's use should be restricted or legislated. Drawing on these contributions, the article identifies questions requiring further research across three thematic areas: knowledge, transparency, and ethics; digital transformation of organisations and societies; and teaching, learning, and scholarly research. The avenues for further research include: identifying skills, resources, and capabilities needed to handle generative AI; examining biases of generative AI attributable to training datasets and processes; exploring business and societal contexts best suited for generative AI implementation; determining optimal combinations of human and generative AI for various tasks; identifying ways to assess accuracy of text produced by generative AI; and uncovering the ethical and legal issues in using generative AI across different contexts

Consumer trust and confidence in the compliance of Islamic banks

Islamic banks compete with traditional (non-Islamic) banks for customers. This article aims to provide insight into why some Muslims choose to bank with Islamic banks in Pakistan, while others do not. Specifically, it addresses the questions: to what extent are trust and confidence active influencers in the decision-making process, are they differentiated or are they one of the same? Also how does the Pakistani collective cultural context further complicate the application of these concepts? For the purposes of this article trust refers to people and their interpersonal or social relations whereas confidence concerns institutions such as banks. Drawing on interviews with Muslim consumers in Pakistan, this study provides further insight into consumer behaviour within financial services and specifically Islamic banking and contributes to our theoretical understanding of the concepts of trust and confidence

Athlete brand construction: A perspective based on fans’ perceptions

Abstract The purpose of this study was to develop a framework for understanding the antecedents and components of athlete brand. Based on a set of 21 interviews conducted in three different countries, a detailed framework is proposed including five antecedents and two components of athlete brand. The antecedents are media (social media, mass media, video games and major sport events), oral communications (word of mouth, and rumors and narratives), impression management, social agents (parents, family members, friends and community), and teams and sport (sport interest, team interest and team geographical location). In turn, the components of athlete brand are related with on-field attributes (behavior, team, achievements, style of play and skills) and off-field attributes (physical attraction, lifestyle, personal appeal, ethnicity and entertainment). Complementarily, these components of athlete brand are proposed to have an impact on fans' loyalty towards the athlete. Implications of these findings for building and managing athlete brand are discussed, and directions for future studies are provided