Effects of enzyme complex SSF (solid state fermentation) in pellet diets for Nile tilapia

The effects of enzyme complex SSF (solid state fermentation) on growth performance and the availability of sucrose and monosaccharides in the chyme of Nile were involved. The study included 360 fish (70g±4.43) in a completely randomized design with six dietary treatments (0, 50, 100, 150, 200 and 250 ppm of SSF) arranged in six replicates, with 10 fish per replicate. Every 15 days, one tilapia of each experimental unit was sacrificed for analyses of carbohydrate in the chyme. On day 60 of the experiment, the performance parameters were measured. There was a linear effect according to treatment for final weight and weight gain. For the other performance parameters, there were no differences. There was quadratic effect for sucrose and glucose in function of the treatment, whereas the fructose levels increased linearly. The addition of 150 ppm of the enzyme complex SSF in the feed improves the performance of Nile tilapia and increases the availability of sucrose and monosaccharides in the chyme

Digestibilidade aparente dos nutrientes e energia de ração suplementada com enzimas digestivas exógenas para juvenis de tambaqui (Colosssoma macropomum Cuvier, 1818) Apparent digestibility of nutrients and crude energy in diets with addition of exogenous digestive enzymes in tambaqui juveniles ( Colosssoma macropomum Cuvier, 1818)

O experimento foi conduzido com o objetivo de avaliar o efeito da adição de um compelxo multienzimático exógeno composto de amilase, protease, lipase e celulase, em rações de juvenis de tambaqui, sobre os coeficientes de digestibilidade aparente (CDa) da proteína bruta (PB), extrato etéreo (EE), carboidratos (ENN) e energia bruta (EB). O delineamento experimental foi inteiramente casualizado com quatro tratamentos (quatro níveis de inclusão de enzimas, 0,0; 0,05; 0,10; e 0,15 %), três repetições (no tempo) e 10 peixes por unidade experimental. Foram utilizados 40 juvenis de tambaqui, com peso médio de 155,0 ± 0,49 g, distribuídos em quatro tanques de alimentação de 500 l, recebendo refeições à vontade das 8 às 12h, a cada hora. Em seguida os animais foram transferidos para coletores de fezes (200 l), onde permaneceram até às 18h, sendo a coleta de dejetos realizada a cada hora. A determinação dos CDa foi realizada pelo método indireto, sendo utilizado como indicador externo 0,5% de óxido de cromo-III (Cr2O3) incorporado à ração. Os resultados demonstraram que a suplementação das dietas com enzimas exógenas para juvenis de tambaqui aumenta a digestibilidade aparente dos nutrientes e energia bruta, no nível de inclusão de 0,05% (P<0,05%).<br>The experiment evaluated the effect of dietary supplementation a multienzyme complex containing amylase, protease, lipase and cellulase for tambaqui juveniles on the apparent digestibility coefficients (ADCs) of crude protein (CP), ether extract (EE), carbohydrates (CHO) and crude energy (CE). A completely randomized experimental design was used with four treatments (level of enzymes 0.0; 0.05; 0.10; and 0.15 %) and three replicates (over time), with ten fish per experimental unity. Forty fish weighting 155.0 ± 0.49g were distributed in four 500 l feeding tanks and were fed hourly, from 8 to 12 AM. After this period, fish were transferred for fecal collection units until 6 PM, where feces were collected hourly. ADCs were evaluated by an indirect method, with 0.5 % III-chromic oxide (Cr2O3) as innert marker. The results showed that supplementary exogenous enzymes on tambaqui diets increased the apparent digestibility of nutrients and crude energy at inclusion level of 0.05% (P<0.05%)