Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging.

Circuit mapping requires knowledge of both structural and functional connectivity between cells. Although optical tools have been made to assess either the morphology and projections of neurons or their activity and functional connections, few probes integrate this information. We have generated a family of photoactivatable genetically encoded Ca(2+) indicators that combines attributes of high-contrast photolabeling with high-sensitivity Ca(2+) detection in a single-color protein sensor. We demonstrated in cultured neurons and in fruit fly and zebrafish larvae how single cells could be selected out of dense populations for visualization of morphology and high signal-to-noise measurements of activity, synaptic transmission and connectivity. Our design strategy is transferrable to other sensors based on circularly permutated GFP (cpGFP)

SPECTROSCOPIC CHARACTERIZATION OF A NIGERIAN STANDARD SAND: IGBOKODA SAND

-1 reveal the presence of quartz and that it is in the crystalline form. Bands at around 800cm -1 -822.79cm -1 and 786.23cm -1, reveal that the silica is in the form of α-Quartz. The Raman spectrum of the sample has two peaks at around 203cm -1 and 463.07cm -1 for A1 vibrational mode characteristic of quartz. These peaks reflect that the sample studied belongs to the low temperature quartz. The occurrence of the Raman shift at 463.07cm -1 supports the observation from XRD studies which establishes that the quartz observed in this work is α-Quartz. The 29 Si NMR spectrum shows a prominent signal at -107.95 ppm correlating to quartz

Invariant Distribution of Promoter Activities in Escherichia coli

Cells need to allocate their limited resources to express a wide range of genes. To understand how Escherichia coli partitions its transcriptional resources between its different promoters, we employ a robotic assay using a comprehensive reporter strain library for E. coli to measure promoter activity on a genomic scale at high-temporal resolution and accuracy. This allows continuous tracking of promoter activity as cells change their growth rate from exponential to stationary phase in different media. We find a heavy-tailed distribution of promoter activities, with promoter activities spanning several orders of magnitude. While the shape of the distribution is almost completely independent of the growth conditions, the identity of the promoters expressed at different levels does depend on them. Translation machinery genes, however, keep the same relative expression levels in the distribution across conditions, and their fractional promoter activity tracks growth rate tightly. We present a simple optimization model for resource allocation which suggests that the observed invariant distributions might maximize growth rate. These invariant features of the distribution of promoter activities may suggest design constraints that shape the allocation of transcriptional resources

Supernova PTF12glz: a possible shock breakout driven through an aspherical wind

We present visible-light and ultraviolet (UV) observations of the supernova PTF12glz. The SN was discovered and monitored in near-UV and R bands as part of a joint GALEX and Palomar Transient Factory campaign. It is among the most energetic Type IIn supernovae observed to date (~10^51erg). If the radiated energy mainly came from the thermalization of the shock kinetic energy, we show that PTF12glz was surrounded by ~1 solar mass of circumstellar material (CSM) prior to its explosive death. PTF12glz shows a puzzling peculiarity: at early times, while the freely expanding ejecta are presumably masked by the optically thick CSM, the radius of the blackbody that best fits the observations grows at ~8000km/s. Such a velocity is characteristic of fast moving ejecta rather than optically thick CSM. This phase of radial expansion takes place before any spectroscopic signature of expanding ejecta appears in the spectrum and while both the spectroscopic data and the bolometric luminosity seem to indicate that the CSM is optically thick. We propose a geometrical solution to this puzzle, involving an aspherical structure of the CSM around PTF12glz. By modeling radiative diffusion through a slab of CSM, we show that an aspherical geometry of the CSM can result in a growing effective radius. This simple model also allows us to recover the decreasing blackbody temperature of PTF12glz. SLAB-Diffusion, the code we wrote to model the radiative diffusion of photons through a slab of CSM and evaluate the observed radius and temperature, is made available on-line.Comment: Sumbitted to ApJ. Comments are welcom

Reliability of a White Striping Scoring System and Description of White Striping Prevalence in Purebred Turkey Lines.

To efficiently meet consumer demands for high-quality lean meat, turkeys are selected for increased meat yield, mainly by increasing breast muscle size and growth efficiency. Over time, this has altered muscle morphology and development rates, which are believed to contribute to the prevalence of myopathies. White striping is a myopathy of economic importance which presents as varying degrees of white striations on the surface of skinless breast muscle and can negatively affect consumer acceptance at the point of sale. Breeding for improved meat quality may be a novel strategy for mitigating the development of white striping in turkey meat; however, it is crucial to have a reliable assessment tool before it can be considered as a phenotype. Six observers used a four-category scoring system (0-3) to score severity in several controlled rounds and evaluate intra- and inter-observer reliability of the scoring system. After sufficient inter-observer reliability (Kendall's W > 0.6) was achieved, 12,321 turkey breasts, from four different purebred lines, were scored to assess prevalence of the condition and analyze its relationship with important growth traits. Overall, the prevalence of white striping (Score > 0) was approximately 88% across all genetic lines studied, with most scores being of moderate-severe severity (Score 1 or 2). As was expected, increased white striping severity was associated with higher slaughter weight, breast weight, and breast meat yield (BMY) within each genetic line. This study highlights the importance of training to improve the reliability of a scoring system for white striping in turkeys and was required to provide an updated account on white striping prevalence in modern turkeys. Furthermore, we showed that white striping is an important breast muscle myopathy in turkeys linked to heavily selected traits such as body weight and BMY. White striping should be investigated further as a novel phenotype in future domestic turkey selection through use of a balanced selection index