1,948 research outputs found
PLASMD BEARING A CDNA COPY OF THE GENOME OF BOVINE VIRAL DIARRHEA VIRUS, CHIMERIC DERIVATIVES THEREOF, AND METHOD OF PRODUCING AN INFECTIOUS BOVINE WRAL DARRHEAVIRUS USING SAD PLASMID
A plasmid bearing a cDNA copy of the genome of bovine viral diarrhea virus (BVDV), chimeric derivatives of the plasmid and a method of producing an infectious bovine viral diarrhea virus using the plasmid are disclosed. The invention relates to a plasmid DNA molecule that replicates easily in E. coli and contains a sufficient portion of the genome of BVDV, cloned as cDNA, to be a suitable template to produce RNA in vitro which, upon transfection into bovine cells, gives rise to infectious BVDV. The BVDV created by the process of the invention can be engineered for use as a vector in many advantageous applications
MetodologĂa y experiencia de FLACAM en la educaciĂłn de agentes del cambio para la sustentabilidad en LatinoamĂ©rica
El següent article ha estat preparat especialment per al desè número de la Revista Sostenible?,
en base a diversos textos precedents, aquĂ ajustats, sintetitzats i actualitzats.
El contingut resumeix els aspectes importants de l’enfocament i l’oferta d’educació superior del
Foro Latinoamericano de Ciencias Ambientales (FLACAM) que, des de 1989, constitueix una
proposta innovadora a Llatinoamèrica per formar agents del canvi cap a la sostenibilitat.
L’article transita des del canvi de paradigma tal com s’ha vingut formalitzant en FLACAM,
mitjançant avenços epistemològics, pedagògics i didà ctics, per donar una semblança abarcativa
però sintètica d’aquest veritable moviment cultural i cientĂfic que des de fa 20 anys va començar
a incidir en la sostenibilitat.El siguiente artĂculo ha sido preparado especialmente para el nĂşmero 10 de la Revista Sostenible?,
en base a varios textos precedentes, aquĂ ajustados, sintetizados y actualizados.
El contenido resume los aspectos salientes del enfoque y la oferta de educaciĂłn superior del Foro
Latinoamericano de Ciencias Ambientales (FLACAM), que desde 1989 constituye una propuesta
innovadora en Latinoamérica para formar agentes del cambio hacia la sustentabilidad.
El artĂculo transita desde el cambio de paradigma tal como se ha venido formalizando en
FLACAM, a través de avances epistemológicos, pedagógicos y didácticos, para dar una semblanza
abarcativa pero sintĂ©tica de este verdadero movimiento cultural y cientĂfico que desde hace 20
años comenzó a incidir en la sustentabilidad.The following article was written specially for Issue 10 of the magazine Sostenible? and is based
on various texts that were adapted, summarized and updated for this piece.
It sums up the main aspects of the approach and higher education courses of the Latin American
Forum of Environmental Sciences (FLACAM). Since 1989, FLACAM has been innovative in
educating agents of change towards sustainability.
The article describes the paradigm shift as it became consolidated in FLACAM, through
epistemological, pedagogical and didactic advances. It also gives a comprehensive but concise
picture of this cultural and scientific movement, which started to have an impact on sustainability
20 years ago.Peer Reviewe
An ab initio path integral Monte Carlo simulation method for molecules and clusters: application to Li_4 and Li_5^+
A novel method for simulating the statistical mechanics of molecular systems
in which both nuclear and electronic degrees of freedom are treated quantum
mechanically is presented. The scheme combines a path integral description of
the nuclear variables with a first-principles adiabatic description of the
electronic structure. The electronic problem is solved for the ground state
within a density functional approach, with the electronic orbitals expanded in
a localized (Gaussian) basis set. The discretized path integral is computed by
a Metropolis Monte Carlo sampling technique on the normal modes of the
isomorphic ring-polymer. An effective short-time action correct to order
is used. The validity and performance of the method are tested in two
small Lithium clusters, namely Li and Li. Structural and electronic
properties computed within this fully quantum-mechanical scheme are presented
and compared to those obtained within the classical nuclei approximation.
Quantum delocalization effects are significant but tunneling turns out to be
irrelevant at low temperatures.Comment: 11 text pages, 7 figures, to be published in J. Chem. Phy
Development of Self Esteem and Learning Skills in Students Participating in the Army Junior Reserve Officers Training Corps (JROTC)
PLASMD BEARING A CDNA COPY OF THE GENOME OF BOVINE VIRAL DIARRHEA VIRUS, CHIMERIC DERIVATIVES THEREOF, AND METHOD OF PRODUCING AN INFECTIOUS BOVINE WRAL DARRHEAVIRUS USING SAD PLASMID
A plasmid bearing a cDNA copy of the genome of bovine viral diarrhea virus (BVDV), chimeric derivatives of the plasmid and a method of producing an infectious bovine viral diarrhea virus using the plasmid are disclosed. The invention relates to a plasmid DNA molecule that replicates easily in E. coli and contains a sufficient portion of the genome of BVDV, cloned as cDNA, to be a suitable template to produce RNA in vitro which, upon transfection into bovine cells, gives rise to infectious BVDV. The BVDV created by the process of the invention can be engineered for use as a vector in many advantageous applications
Image patch analysis of sunspots and active regions. II. Clustering via matrix factorization
Separating active regions that are quiet from potentially eruptive ones is a
key issue in Space Weather applications. Traditional classification schemes
such as Mount Wilson and McIntosh have been effective in relating an active
region large scale magnetic configuration to its ability to produce eruptive
events. However, their qualitative nature prevents systematic studies of an
active region's evolution for example. We introduce a new clustering of active
regions that is based on the local geometry observed in Line of Sight
magnetogram and continuum images. We use a reduced-dimension representation of
an active region that is obtained by factoring the corresponding data matrix
comprised of local image patches. Two factorizations can be compared via the
definition of appropriate metrics on the resulting factors. The distances
obtained from these metrics are then used to cluster the active regions. We
find that these metrics result in natural clusterings of active regions. The
clusterings are related to large scale descriptors of an active region such as
its size, its local magnetic field distribution, and its complexity as measured
by the Mount Wilson classification scheme. We also find that including data
focused on the neutral line of an active region can result in an increased
correspondence between our clustering results and other active region
descriptors such as the Mount Wilson classifications and the value. We
provide some recommendations for which metrics, matrix factorization
techniques, and regions of interest to use to study active regions.Comment: Accepted for publication in the Journal of Space Weather and Space
Climate (SWSC). 33 pages, 12 figure
Image patch analysis of sunspots and active regions. I. Intrinsic dimension and correlation analysis
The flare-productivity of an active region is observed to be related to its
spatial complexity. Mount Wilson or McIntosh sunspot classifications measure
such complexity but in a categorical way, and may therefore not use all the
information present in the observations. Moreover, such categorical schemes
hinder a systematic study of an active region's evolution for example. We
propose fine-scale quantitative descriptors for an active region's complexity
and relate them to the Mount Wilson classification. We analyze the local
correlation structure within continuum and magnetogram data, as well as the
cross-correlation between continuum and magnetogram data. We compute the
intrinsic dimension, partial correlation, and canonical correlation analysis
(CCA) of image patches of continuum and magnetogram active region images taken
from the SOHO-MDI instrument. We use masks of sunspots derived from continuum
as well as larger masks of magnetic active regions derived from the magnetogram
to analyze separately the core part of an active region from its surrounding
part. We find the relationship between complexity of an active region as
measured by Mount Wilson and the intrinsic dimension of its image patches.
Partial correlation patterns exhibit approximately a third-order Markov
structure. CCA reveals different patterns of correlation between continuum and
magnetogram within the sunspots and in the region surrounding the sunspots.
These results also pave the way for patch-based dictionary learning with a view
towards automatic clustering of active regions.Comment: Accepted for publication in the Journal of Space Weather and Space
Climate (SWSC). 23 pages, 11 figure
Construction supply chain modeling : a research review and interdisciplinary research agenda
Supply chain management has emerged as a popular and useful concept in the construction industry and research community since the mid 1990s. Research in construction supply chain management draws from a broad range of disciplines, notably: (1) Industrial organization economics to better understand market structure and forces and their effect on firm and supply chain behavior and (2) Analytic modeling of supply chains to improve supply chain performance along metrics such as speed, cost, reliability, quality, etc. Both industrial organization and analytic modeling provide useful but ultimately incomplete perspectives and prescriptions for construction supply chain management. As such, this paper proposes development of an interdisciplinary research agenda that draws from both fields. Towards that agenda, a review of research is presented to introduce the main ideas, relevant literature, and theory and methods in each of the two areas. From these independent reviews, applications that could benefit from a combined perspective are identified and used as a basis for development of an interdisciplinary research agenda.<br /
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