Sanitizing the fortress: protection of ant brood and nest material by worker antibiotics

Social groups are at particular risk for parasite infection, which is heightened in eusocial insects by the low genetic diversity of individuals within a colony. To combat this, adult ants have evolved a suite of defenses to protect each other, including the production of antimicrobial secretions. However, it is the brood in a colony that are most vulnerable to parasites because their individual defenses are limited, and the nest material in which ants live is also likely to be prone to colonization by potential parasites. Here, we investigate in two ant species whether adult workers use their antimicrobial secretions not only to protect each other but also to sanitize the vulnerable brood and nest material. We find that, in both leaf-cutting ants and weaver ants, the survival of the brood was reduced and the sporulation of parasitic fungi from them increased, when the workers nursing them lacked functional antimicrobial-producing glands. This was the case for both larvae that were experimentally treated with a fungal parasite (Metarhizium) and control larvae which developed infections of an opportunistic fungal parasite (Aspergillus). Similarly, fungi were more likely to grow on the nest material of both ant species if the glands of attending workers were blocked. The results show that the defense of brood and sanitization of nest material are important functions of the antimicrobial secretions of adult ants and that ubiquitous, opportunistic fungi may be a more important driver of the evolution of these defenses than rarer, specialist parasites

Revealing New Physical Structures in the Supernova Remnant N63A through Chandra Imaging Spectroscopy

We present Chandra X-ray observations of the supernova remnant (SNR) N63A in the Large Magellanic Cloud (LMC). N63A, one of the brightest LMC remnants, is embedded in an H II region and probably associated with an OB association. The optical remnant consists of three lobes of emission contained within the approximately three times larger X-ray remnant. Our Chandra data reveal a number of new physical structures in N63A. The most striking of these are the several ``crescent''-shaped structures located beyond the main shell that resemble similar features seen in the Vela SNR. In Vela, these have been interpreted as arising from high speed clumps of supernova ejecta interacting with the ambient medium. Another distinct feature of the remnant is a roughly triangular ``hole'' in the X-ray emission near the location of the optical lobes and the brightest radio emission. X-ray spectral analysis shows that this deficit of emission is a result of absorption by an intervening dense cloud with a mass of ~450 M_sun that is currently being engulfed by the remnant's blast wave. We also find that the rim of the remnant, as well as the crescent-shaped features, have considerably softer X-ray spectra than the interior. Limits on hard X-ray emission rule out a young, energetic pulsar in N63A, but the presence of an older or less active one, powering a wind nebula with a luminosity less than ~4e10^34 erg/s, is allowed.Comment: 18 pages, 5 figures (2 color), accepted for publication in Ap

First exit times and residence times for discrete random walks on finite lattices

In this paper, we derive explicit formulas for the surface averaged first exit time of a discrete random walk on a finite lattice. We consider a wide class of random walks and lattices, including random walks in a non-trivial potential landscape. We also compute quantities of interest for modelling surface reactions and other dynamic processes, such as the residence time in a subvolume, the joint residence time of several particles and the number of hits on a reflecting surface.Comment: 19 pages, 2 figure

Short-term heat stress results in diminution of bacterial symbionts but has little effect on life history in adult female citrus mealybugs

Mealybugs are sap-feeding insect pests that pose a serious threat to horticulture. The citrus mealybug, Planococcus citri (Risso) (Hemiptera: Pseudococcidae), like most other mealybug species, harbours two obligate maternally transmitted bacterial endosymbionts, which are essential for nutrient acquisition and host survival. These are ‘Candidatus Tremblaya princeps’, a member of the β-Proteobacteria, and ‘Candidatus Moranella endobia’, a member of the γ-Proteobacteria. The density of symbionts in the hosts is now understood to be dynamic, being influenced by the age and gender of the host and by environmental conditions during development. Here, we examine the impact of short-term heat stress treatment on the obligate symbionts and life-history parameters of P. citri, using qPCR to measure changes in symbiont density. Heat stress killed juveniles and adult males, and significantly reduced levels of ‘Ca. Moranella endobia’ and ‘Ca. Tremblaya princeps’ in adult females. However, adult females were resilient to this and it did not affect their fecundity or brood survival, although the sex ratio of their brood was slightly, but significantly, more female biased. Our results suggest that ‘Ca. Tremblaya princeps’ and ‘Ca. Moranella endobia’ are not as essential to the survival of adult mealybugs as they are to the survival of immature mealybugs and that sub-lethal heat treatment alone is unlikely to be effective as a disinfestation tactic

VARIABLY CURING RESINS FOR MOUNTING METALLOGRAPHIC SAMPLES

In the past, heat-and-pressure-curing resins have been used almost exclusively as mounting materials for metallographic samples. Now, however, certain polyester resins, which are curable at room temperature, are equally as good for this purpose, and with their versatility they adapt readily to a variety of mounting conditions. A study of the epoxies and polyesters was made to determine which type of resin would satisfy the desirable properties of a metallographic mount. Four polyester resins were selected and investigated intensively to ascertain the variables associated with their curing processes. The results are compared with the standard thermosetting mounting material, Bakelite. (auth

Type-Inference Based Short Cut Deforestation (nearly) without Inlining

Deforestation optimises a functional program by transforming it into another one that does not create certain intermediate data structures. In [ICFP'99] we presented a type-inference based deforestation algorithm which performs extensive inlining. However, across module boundaries only limited inlining is practically feasible. Furthermore, inlining is a non-trivial transformation which is therefore best implemented as a separate optimisation pass. To perform short cut deforestation (nearly) without inlining, Gill suggested to split definitions into workers and wrappers and inline only the small wrappers, which transfer the information needed for deforestation. We show that Gill's use of a function build limits deforestation and note that his reasons for using build do not apply to our approach. Hence we develop a more general worker/wrapper scheme without build. We give a type-inference based algorithm which splits definitions into workers and wrappers. Finally, we show that we can deforest more expressions with the worker/wrapper scheme than the algorithm with inlining

Computational requirements of the virtual patient

Medical visualization in a hospital can be used to aid training, diagnosis, and pre- and intra-operative planning. In such an application, a virtual representation of a patient is needed that is interactive, can be viewed in three dimensions (3D), and simulates physiological processes that change over time. This paper highlights some of the computational challenges of implementing a real time simulation of a virtual patient, when accuracy can be traded-off against speed. Illustrations are provided using projects from our research based on Grid-based visualization, through to use of the Graphics Processing Unit (GPU)

Master Operators Govern Multifractality in Percolation

Using renormalization group methods we study multifractality in percolation at the instance of noisy random resistor networks. We introduce the concept of master operators. The multifractal moments of the current distribution (which are proportional to the noise cumulants $C_R^{(l)} (x, x^\prime)$ of the resistance between two sites x and $x^\prime$ located on the same cluster) are related to such master operators. The scaling behavior of the multifractal moments is governed exclusively by the master operators, even though a myriad of servant operators is involved in the renormalization procedure. We calculate the family of multifractal exponents ${\psi_l}$ for the scaling behavior of the noise cumulants, $C_R^{(l)} (x, x^\prime) \sim | x - x^\prime |^{\psi_l /\nu}$, where $\nu$ is the correlation length exponent for percolation, to two-loop order.Comment: 6 page

Finite-element analysis of contact between elastic self-affine surfaces

Finite element methods are used to study non-adhesive, frictionless contact between elastic solids with self-affine surfaces. We find that the total contact area rises linearly with load at small loads. The mean pressure in the contact regions is independent of load and proportional to the rms slope of the surface. The constant of proportionality is nearly independent of Poisson ratio and roughness exponent and lies between previous analytic predictions. The contact morphology is also analyzed. Connected contact regions have a fractal area and perimeter. The probability of finding a cluster of area $a_c$ drops as $a_c^{-\tau}$ where $\tau$ increases with decreasing roughness exponent. The distribution of pressures shows an exponential tail that is also found in many jammed systems. These results are contrasted to simpler models and experiment.Comment: 13 pages, 15 figures. Replaced after changed in response to referee comments. Final two figures change

Intermittent random walks for an optimal search strategy: One-dimensional case

We study the search kinetics of an immobile target by a concentration of randomly moving searchers. The object of the study is to optimize the probability of detection within the constraints of our model. The target is hidden on a one-dimensional lattice in the sense that searchers have no a priori information about where it is, and may detect it only upon encounter. The searchers perform random walks in discrete time n=0,1,2, ..., N, where N is the maximal time the search process is allowed to run. With probability \alpha the searchers step on a nearest-neighbour, and with probability (1-\alpha) they leave the lattice and stay off until they land back on the lattice at a fixed distance L away from the departure point. The random walk is thus intermittent. We calculate the probability P_N that the target remains undetected up to the maximal search time N, and seek to minimize this probability. We find that P_N is a non-monotonic function of \alpha, and show that there is an optimal choice \alpha_{opt}(N) of \alpha well within the intermittent regime, 0 < \alpha_{opt}(N) < 1, whereby P_N can be orders of magnitude smaller compared to the "pure" random walk cases \alpha =0 and \alpha = 1.Comment: 19 pages, 5 figures; submitted to Journal of Physics: Condensed Matter; special issue on Chemical Kinetics Beyond the Textbook: Fluctuations, Many-Particle Effects and Anomalous Dynamics, eds. K.Lindenberg, G.Oshanin and M.Tachiy