Different methods for administering 17β-estradiol to ovariectomized rats result in opposite effects on ischemic brain damage

<p>Abstract</p> <p>Background</p> <p>Numerous stroke studies have controversially shown estrogens to be either neuroprotective or neurodamaging. The discordant results observed in rat brain ischemia models may be a consequence of discrepancies in estrogen administration modes resulting in plasma concentration profiles far from those intended. To test this hypothesis we reproduced in detail and extended an earlier study from our lab using a different mode of 17β-estradiol administration; home-made silastic capsules instead of commercial slow-release 17β-estradiol pellets. Four groups of female rats (n = 12) were ovariectomized and administered 17β-estradiol or placebo via silastic capsules. All animals underwent MCAo fourteen days after ovariectomy and were sacrificed three days later.</p> <p>Results</p> <p>In contrast to our earlier results using the commercial pellets, the group receiving 17β-estradiol during the entire experiment had significantly smaller lesions than the group receiving placebo (mean ± SEM: 3.85 ± 0.70% versus 7.15 ± 0.27% of total slice area, respectively; p = 0.015). No significant neuroprotection was found when the 17β-estradiol was administered only during the two weeks before or the three days immediately after MCAo.</p> <p>Conclusions</p> <p>The results indicate that different estrogen treatment regimens result in diametrically different effects on cerebral ischemia. Thus the effects of estrogens on ischemic damage seem to be concentration-related, with a biphasic, or even more complex, dose-response relation. These findings have implications for the design of animal experiments and also have a bearing on the estrogen doses used for peri-menopausal hormone replacement therapy.</p

Effects of the background radiation on radio pulsar and supernova remnant searches and the birth rates of these objects

In different directions of the Galaxy the Galactic background radio radiation and radiation of complex star formation regions which include large number of OB associations have different influences on radio pulsar (PSR) and supernova remnant (SNR) searches. In this work we analyse the effects of these background radiations on the observations of PSRs at 1400 MHz and SNRs at 1000 MHz. In the interval l=0$^o$$\pm60^o$ the PSRs with flux F$_{1400}$$>$0.2 mJy and the SNRs with surface brightness $\Sigma$$>10^{-21}$ Wm$^{-2}$Hz$^{-1}$sr$^{-1}$ are observable for all values of l and b. All the SNRs with $\Sigma$$>3\times10^{-22}$ Wm$^{-2}$Hz$^{-1}$sr$^{-1}$ can be observed in the interval 60$^o$$<$l$<300^o$. We have examined samples of PSRs and SNRs to estimate the birth rates of these objects in the region up to 3.2 kpc from the Sun and also in the Galaxy. The birth rate of PSRs is about one in 200 years and the birth rate of SNRs is about one in 65 years in our galaxy.Comment: revised versio

Improved flux limits for neutrinos with energies above 1022^{22} eV from observations with the Westerbork Synthesis Radio Telescope

Particle cascades initiated by ultra-high energy (UHE) neutrinos in the lunar regolith will emit an electromagnetic pulse with a time duration of the order of nano seconds through a process known as the Askaryan effect. It has been shown that in an observing window around 150 MHz there is a maximum chance for detecting this radiation with radio telescopes commonly used in astronomy. In 50 hours of observation time with the Westerbork Synthesis Radio Telescope array we have set a new limit on the flux of neutrinos, summed over all flavors, with energies in excess of $4\times10^{22}$ eV.Comment: Submitted to Phys. Rev. Let

Radio Spectra of Giant Radio Galaxies from RATAN-600 Data

Measurements of the flux densities of the extended components of seven giant radio galaxies obtained using the RATAN-600 radio telescope at wavelengths of 6.25 and 13 cm ar e presented. The spectra of components of these radio galaxies are constructed using these new RA TAN-600 data together with data from the WENSS, NVSS, and GB6 surveys. The spectral indices in the stu died frequency range are calculated, and the need for detailed estimates of the integrated contributi on of such objects to the background emission is demonstrated.Comment: 7 pages, 2 figures, 5 table

HST/NICMOS Imaging of Disks and Envelopes Around Very Young Stars

We present HST/NICMOS observations with 0.1" (15 AU) resolution of six young stellar objects in the Taurus star-formation region. The targets of our survey are three Class I IRAS sources (IRAS 04016+2610, IRAS 04248+2612, and IRAS 04302+2247) and three low-luminosity stars (DG Tau B, Haro 6-5B, and CoKu Tau/1) associated with Herbig Haro jets. The broad-band images show that the near-infrared radiation from these sources is dominated by light scattered from dusty circumstellar material distributed in a region 10 - 15 times the size of our solar system. Although the detailed morphologies of the individual objects are unique, the observed young stellar objects share common features. All of the circumstellar reflection nebulae are crossed by dark lanes from 500 - 900 AU in extent and from less than 50 to 350 AU in apparent thickness. The absorption lanes extend perpendicular to known optical and millimeter outflows in these sources. We interpret the dark lanes as optically thick circumstellar disks seen in silhouette against bright reflection nebulosity. The bipolar reflection nebulae extending perpendicular to the dust lanes appear to be produced by scattering from the upper and lower surfaces of the disks and from dusty material within or on the walls of the outflow cavities. Out of five objects in which the central source is directly detected, two are found to be subarcsecond binaries. This mini-survey is the highest resolution near-infrared study to date of circumstellar environments around solar-type stars with age <= 1 Myr.Comment: 34 pages, 4 figures; also available at http://spider.ipac.caltech.edu/staff/brandner/topics/disks/disks.html ; accepted for publication in AJ (March 1999 issue

The Discovery of an Anomalous X-ray Pulsar in the Supernova Remnant Kes 73

We report the discovery of pulsed X-ray emission from the compact source 1E 1841-045, using data obtained with the Advanced Satellite for Cosmology and Astrophysics. The X-ray source is located in the center of the small-diameter supernova remnant (SNR) Kes 73 and is very likely to be the compact stellar-remnant of the supernova which formed Kes 73. The X-rays are pulsed with a period of ~ 11.8 s, and a sinusoidal modulation of roughly 30 %. We interpret this modulation to be the rotation period of an embedded neutron star, and as such would be the longest spin period for an isolated neutron star to-date. This is especially remarkable since the surrounding SNR is very young, at ~ 2000 yr old. We suggest that the observed characteristics of this object are best understood within the framework of a neutron star with an enormous dipolar magnetic field, B ~ 8x10^14 G

Time- and compartment-resolved proteome profiling of the extracellular niche in lung injury and repair

The extracellular matrix (ECM) is a key regulator of tissue morphogenesis and repair. However, its composition and architecture are not well characterized. Here, we monitor remodeling of the extracellular niche in tissue repair in the bleomycin-induced lung injury mouse model. Mass spectrometry quantified 8,366 proteins from total tissue and bronchoalveolar lavage fluid (BALF) over the course of 8 weeks, surveying tissue composition from the onset of inflammation and fibrosis to its full recovery. Combined analysis ofproteome, secretome, and transcriptome highlighted post-transcriptional events during tissue fibrogenesis and defined the composition of airway epithelial lining fluid. To comprehensively characterize the ECM, we developed a quantitative detergent solubility profiling (QDSP) method, which identified Emilin-2 and collagen-XXVIII as novel constituents of the provisional repair matrix. QDSP revealed which secreted proteins interact with the ECM, and showed drastically altered association of morphogens to the insoluble matrix upon injury. Thus, our proteomic systems biology study assigns proteins to tissue compartments and uncovers their dynamic regulation upon lung injury and repair, potentially contributing to the development of anti-fibrotic strategies