Rapid range expansion in the Great Plains narrow-mouthed toad (Gastrophryne olivacea) and a revised taxonomy for North American microhylids

We investigated genetic variation within the Great Plains narrow-mouthed toad, Gastrophryne olivacea, across its geographic range in the United States and Mexico. An analysis of mitochondrial DNA (mtDNA) from 105 frogs revealed remarkably low levels of genetic diversity in individuals inhabiting the central United States and northern Mexico. We found that this widespread matrilineal lineage is divergent (ca. 2% in mtDNA) from haplotypes that originate from the western United States and western coast of Mexico. Using a dataset that included all five species of Gastrophryne and both species of the closely related genus Hypopachus, we investigated the phylogenetic placement of G. olivacea. This analysis recovered strong support that G. olivacea, the tropically distributed G. elegans, and the temperately distributed G. carolinensisconstitute a monophyletic assemblage. However, the placement of G. pictiventris and G. usta render Gastrophryne paraphyletic with respect to Hypopachus. To complement our mitochondrial analysis, we examined a small fragment of nuclear DNA and recovered consistent patterns. In light of our findings we recommend (1) the resurrection of the nomen G. mazatlanensis Taylor (1943) for the disjunct western clade of G. olivaceaand (2) the tentative placement of G. pictiventris and G. usta in Hypopachus. To explore possible scenarios leading to low levels of genetic diversity in G. olivacea, we used mismatch distributions and Bayesian Skyline plots to examine historic population expansion and demography. Collectively these analyses suggest that G. olivacea rapidly expanded in effective population size and geographic range during the late Pleistocene or early Holocene. This hypothesis is consistent with fossil data from northern localities and contemporary observations that suggest ongoing northern expansion. Given our findings, we suspect that the rapid range expansion of G. olivacea may have been facilitated by ecological associations with open habitats and seasonal water bodies

A 2D multiwavelength study of the ionized gas and stellar population in the Giant HII Region NGC 588

We present an analysis of NGC588 based on IFS data with PMAS, together with Spitzer images at 8 mi and 24 mi. The extinction distribution in the optical shows complex structure, with maxima correlating in position with those of the emission at 24 mi and 8 mi. The Ha luminosity absorbed by the dust within the GHIIR reproduces the structure observed in the 24 mi image, supporting the use of this band as a tracer of recent star formation. A velocity difference of ~50 km/s was measured between the areas of high and low surface brightness, which would be expected if NGC588 were an evolved GHIIR. Line ratios used in the BPT diagnostic diagrams show a larger range of variation in the low surface brightness areas. The ranges are ~0.5 to 1.2 dex for [NII]/Ha, 0.7 to 1.7 dex for [SII]/Ha, and 0.3 to 0.5 dex for [OIII]/Hb. Ratios corresponding to large ionization parameter (U) are found between the peak of the emission in Hb and the main ionizing source decreasing radially outwards within the region. Differences between the integrated and local values of the U tracers can be as high as ~0.8 dex. [OII]/Hb and [OIII]/[OII] yield similar local values for U and consistent with those expected from the integrated spectrum of an HII region ionized by a single star. The ratio [SII]/Ha departs significantly from the range predicted by this scenario, indicating the complex ionization structure in GHIIRs. There is a significant scatter in derivations of Z using strong line tracers as a function of position, caused by variations in the degree of ionization. The scatter is smaller for N2O3 which points to this tracer as a better Z tracer than N2. The comparison between integrated and local line ratio values indicates that measurements of the line ratios of GHIIR in galaxies at distances >~25 Mpc may be dominated by the ionization conditions in their low surface brightness areas.AM-I, EP-M and JMV acknowledge partial funding through research projects AYA2007-67965-C03-02 from the Spanish PNAYA and CSD2006-00070 1st Science with GTC of the MICINN. MR is supported by a Marie Curie Intra European Fellowship within the 7th European Community Framework Programme. CK, as a Humboldt Fellow, acknowledges support from the Alexander von Humboldt Foundation, Germany

Optical Properties of Deep Ice at the South Pole - Absorption

We discuss recent measurements of the wavelength-dependent absorption coefficients in deep South Pole ice. The method uses transit time distributions of pulses from a variable-frequency laser sent between emitters and receivers embedded in the ice. At depths of 800 to 1000 m scattering is dominated by residual air bubbles, whereas absorption occurs both in ice itself and in insoluble impurities. The absorption coefficient increases approximately exponentially with wavelength in the measured interval 410 to 610 nm. At the shortest wavelength our value is about a factor 20 below previous values obtained for laboratory ice and lake ice; with increasing wavelength the discrepancy with previous measurements decreases. At around 415 to 500 nm the experimental uncertainties are small enough for us to resolve an extrinsic contribution to absorption in ice: submicron dust particles contribute by an amount that increases with depth and corresponds well with the expected increase seen near the Last Glacial Maximum in Vostok and Dome C ice cores. The laser pulse method allows remote mapping of gross structure in dust concentration as a function of depth in glacial ice.Comment: 26 pages, LaTex, Accepted for publication in Applied Optics. 9 figures, not included, available on request from [email protected]

The optical module of the Baikal deep underwater neutrino telescope

A deep underwater Cherenkov telescope has been operating since 1993 in stages of growing size at 1.1 km depth in Lake Baikal. The key component of the telescope is the Optical Module (OM) which houses the highly sensitive phototube QUASAR-370. We describe design and parameters of the QUASAR-370, the layout of the optical module, the front-end electronics and the calibration procedures, and present selected results from the five-year operation underwater. Also, future developments with respect to a telescope consisting from several thousand OMs are discussed.Comment: 30 pages, 24 figure

BAIKAL experiment: status report

We review the present status of the Baikal Neutrino Project and present the results obtained with the deep underwater neutrino telescope NT-200.Comment: 4 pages, 3 figures. Presented at TAUP 2001 (7th international workshop on Topics in Astroparticle and Underground Physics), Sep. 2001, Laboratori Nazionali del Gran Sasso, Assergi, Ital

Spinal cord from body donors is suitable for multicolor immunofluorescence

Immunohistochemistry is a powerful tool for studying neuronal tissue from humans at the molecular level. Obtaining fresh neuronal tissue from human organ donors is difficult and sometimes impossible. In anatomical body donations, neuronal tissue is dedicated to research purposes and because of its easier availability, it may be an alternative source for research. In this study, we harvested spinal cord from a single organ donor 2 h (h) postmortem and spinal cord from body donors 24, 48, and 72 h postmortem and tested how long after death, valid multi-color immunofluorescence or horseradish peroxidase (HRP) immunohistochemistry is possible. We used general and specific neuronal markers and glial markers for immunolabeling experiments. Here we showed that it is possible to visualize molecularly different neuronal elements with high precision in the body donor spinal cord 24 h postmortem and the quality of the image data was comparable to those from the fresh organ donor spinal cord. High-contrast multicolor images of the 24-h spinal cords allowed accurate automated quantification of different neuronal elements in the same sample. Although there was antibody-specific signal reduction over postmortem intervals, the signal quality for most antibodies was acceptable at 48 h but no longer at 72 h postmortem. In conclusion, our study has defined a postmortem time window of more than 24 h during which valid immunohistochemical information can be obtained from the body donor spinal cord. Due to the easier availability, neuronal tissue from body donors is an alternative source for basic and clinical research