In silico analysis of compounds characterized from ethanolic extract of Cucurbita pepo with NF-κB-inhibitory potential

NF-κB controls cellular growth properties of cells and its regulation is key to the management of disease like cancer. Although plant-derived bioactives have been reported to inhibit NF-κB, there is limited knowledge on the interactions between the phytochemicals and NF-κB. In this study, we identified the phytochemicals in ethanolic extract of Cucurbita pepo using Gas Chromatography-Mass Spectroscopy technique and used in silico approach to understand the interaction between the identified phytochemicals and NF-κB using Molegro Virtual Docker. The docking algorithm showed that nine phytochemicals fit well into the pocket on NF-κB.. Our analysis showed that Lys144 is a prominent residue by involving in the binding of 9-octadecenoic acid (Z)-, methyl ester, hexadecanoic acid, methyl ester and octadecanoic acid, methyl ester with the moldock score of -55.5264, -57.4634 and -61.1258 respectively. Hence, the binding of these phytochemicals to NF-κB could be responsible for the anti-inflammatory and anti-cancer property of C. pepo

Hesperidin prevents lipopolysaccharide-induced endotoxicity in rats

Context: Lipopolysaccharide (LPS) is a major trigger of septic shock resulting in multiple organ damage through excessive stimulation of the host’s immune cells resulting in the release of cytokines. Previous studies have shown that hesperidin has several beneficial properties against inflammation and oxidative stress. Objective: The influence of hesperidin on endotoxemia, endothelial dysfunction, inflammation, and oxidative stress was investigated using a murine model of sepsis. Materials and methods: Rats were pretreated for 15 d with three doses (50 mg/kg, 100 mg/kg, and 200 mg/kg) of hesperidin prior to LPS administration. Afterwards, the levels of biomarkers of endotoxemia, endothelial dysfunction, and oxidative stress were assessed. Reverse transcriptase PCR technique was used to assess the expression of hepatic proinflammatory cytokines. Results: Hesperidin pretreatment significantly (p < 0.05) reduced circulating endotoxin, as well as the levels of bactericidal permeability increasing protein and procalcitonin, and the associated endothelial dysfunction by reducing the levels of plasma soluble intercellular adhesion molecules 1 and inducible nitric oxide (iNO) synthase. There was also down-regulation of the expression of gene for interleukin 1α, interleukin 1β, interleukin 1 receptor, interleukin 6, and tumor necrosis factor α (TNFα) in the liver of rats treated with LPS as a result of hesperidin pretreatment. Hesperidin also showed anti-oxidative properties through the significant (p < 0.05) reduction of NO, hydroperoxides, and thiobarbituric acid reactive substances and increase of glutathione, glutathione reductase, glutathione peroxidase, and glutathione-S-transferase in the organs. Conclusion: Different doses of hesperidin can prevent endotoxemia-induced oxidative stress as well as inflammatory and endothelial perturbation in rats when administered for as few as 15 d before exposure to endotoxin

Trend Analysis of Teenage Pregnancy in Nigeria (1961-2013): How Effective is the Contraceptive Use Campaign

Teenage pregnancy (TP) is a recurrent global and public health problem. It poses both social and health challenges. Considering the massive campaign on the use of modern contraceptives to prevent TP in recent decades, we assessed trends in TP in Nigeria between 1961 and 2013. Pregnancy and contraception history of 70,811 women who were at least 20 years old when the Nigerian DHS was conducted in 1990, 2003, 2008, and 2013 respectively were used for the study, and descriptive statistics, time analysis techniques and multiple logistic regression were used to analyze the data at 5% significance level. The overall prevalence of TP between 1961 and 2013 was 49.5% which fluctuated insignificantly during the studied period. The TP prevalence among women who entered adulthood in 1961 was 39.2%; it peaked in 1978 at 58.9% before its unsteady decline to 39.6% in 2012, and then rose sharply to 55.6% in 2013. We predicted TP prevalence as 49.0%, 49.9% and 51.0% in 2014, 2015 and 2016 respectively. The odds of TP were over 4 times higher in the North East and 5 times higher in the North West than in the South West. Teenagers with no education had higher odds of TP and it was higher among teenagers from the poorest households (OR=5.64, 95% CI: 5.36-5.94). Rather than reducing with the worldwide acknowledged increase in contraceptive campaigns, TP increased over the years studied. As far as TP is concerned in Nigeria, the impact of the campaign on MC use is far from being effective. To achieve the objective of fewer TPs, fewer resources should be spent on access to contraception and instead diverted to areas more likely to achieve results such as improvements in educational achievement amongst girls

Optical boundaries for LED-based indoor positioning system

Overlap of footprints of light emitting diodes (LEDs) increases the positioning accuracy of wearable LED indoor positioning systems (IPS) but such an approach assumes that the footprint boundaries are defined. In this work, we develop a mathematical model for defining the footprint boundaries of an LED in terms of a threshold angle instead of the conventional half or full angle. To show the effect of the threshold angle, we compare how overlaps and receiver tilts affect the performance of an LED-based IPS when the optical boundary is defined at the threshold angle and at the full angle. Using experimental measurements, simulations, and theoretical analysis, the effect of the defined threshold angle is estimated. The results show that the positional time when using the newly defined threshold angle is 12 times shorter than the time when the full angle is used. When the effect of tilt is considered, the threshold angle time is 22 times shorter than the full angle positioning time. Regarding accuracy, it is shown in this work that a positioning error as low as 230 mm can be obtained. Consequently, while the IPS gives a very low positioning error, a defined threshold angle reduces delays in an overlap-based LED IPS

Plasma lipid profile, atherogenic and coronary risk indices in some residents of Abeokuta in south-western Nigeria

The incidence of chronic degenerative diseases like stroke and myocardial infarction in African subpopulations is reported to be increasing. In view of the association between dyslipidemia and these chronic degenerative diseases, we investigated some well-established cardiovascular risk factors (plasma cholesterol and its fractions as high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglyceride, adiposity and blood pressure) in 92 subjects (43 males and 49 females) in Abeokuta, Nigeria. LDL cholesterol was significantly higher in the women compared with men (128.58mg/dl vs 108.73mg/dl; p = 0.028). Total cholesterol, although slightly higher in women, was not significantly different from that of men (155.71mg/dl vs 147.26mg/dl). HDL cholesterol and triglyceride were not significantly different between the two sexes, although women tended to have lower HDL cholesterol when compared with men. LDL/HDL cholesterol and total cholesterol/HDL cholesterol were significantly higher in women compared with men (4.20 vs 2.97; p = 0.004; 5.03 vs 4.06; p = 0.024, respectively). Systolic blood pressure was higher in men compared with women (117.58 vs 104.84; p = 0.002). Results indicate that the association between cholesterol and chronic degenerative diseases is continuous with no single cholesterol level separating those who are at high risk from those who are not. Rather in defining cardiovascular risks in African populations, the ratios LDL/HDL cholesterol and total cholesterol/HDL cholesterol should be considered. It might be appropriate at this time in Nigeria to consider physical activity and pharmacological interventions in lowering blood lipids

Modeling of the Glycolysis Pathway in Plasmodium falciparum using Petri Nets

Malaria is one of the deadly diseases, which affects a large number of the world’s population. The Plasmodium falciparum parasite during erythrocyte stages produces its energy mainly through anaerobic glycolysis, with pyruvate being converted into lactate. The glycolysis metabolism in P. falci-parum is one of the important metabolic pathways of the parasite because the parasite is entirely dependent on it for energy. Also, several glycolytic enzymes have been proposed as drug targets. Petri nets (PNs) have been recognized as one of the important models for representing biological pathways. In this work, we built a qualitative PN model for the glycolysis pathway in P. falciparum and analyzed the model for its structural and quantitative properties using PN theory. From PlasmoCyc files, a total of 11 reactions were extracted; 6 of these were reversible and 5 were irreversible. These reactions were catalyzed by a total number of 13 enzymes. We extracted some of the essential reactions in the pathway using PN model, which are the possible drug targets without which the pathway cannot function. This model also helps to improve the understanding of the biological processes within this pathway