Comparative analyses on medium optimization using one-factor-at-a-time, response surface methodology, and artificial neural network for lysine–methionine biosynthesis by Pediococcus pentosaceus RF-1

Optimization strategy that encompassed one-factor-at-a-time (OFAT), response surface methodology (RSM), and artificial neural network method was implemented during medium formulation with specific aim for lysine-methionine biosynthesis employing a newly isolated strain of Pediococcus pentosaceus RF-1. OFAT technique was used in the preliminary screening of factors (molasses, nitrogen sources, fish meal, glutamic acid and initial medium pH) before proceeded to optimization study. Implementation of central composite design of experiment subsequently generated 30 experimental runs based on four factors (molasses, fish meal, glutamic acid, and initial medium pH). From RSM analysis, a quadratic polynomial model can be devoted to describing the relationship between various medium components and responses. It also suggested that using molasses (9.86 g/L), fish meal (10.06 g/L), glutamic acid (0.91 g/L), and initial medium pH (5.30) would enhance the biosynthesis of lysine (15.77 g/L) and methionine (4.21 g/L). Alternatively, a three-layer neural network topography at 4-5-2 predicted a further improvement in the biosynthesis of lysine (16.52 g/L) and methionine (4.53 g/L) by using formulation composed of molasses (10.02 g/L), fish meal (18.00 g/L), and glutamic acid (1.17 g/L) with initial medium pH (4.26), respectively

L'authentification rapide des poulets de chair sous label: distinction entre poulets issus de souches à croissance lente ou rapide par la spectrometrie dans le proche infrarouge. Analytical methods for the authentification of agro-food products. Gembloux (Belgium). 20 Oct 1999

Rapid authentification of certified chicken meat: distinguishing slow and fast growing chicken strains using near infrared reflectance spectroscopy. Chicken meat market is characterized by numerous quality marks in Belgium and in Europe: ""Label de Qualite Wallon"" in Belgium, ""Label Rouge"" in France, Labels for geographical origin, organic agriculture, ... The authors have already tested, Near Infrared Reflectance Spectroscopy (NIRS) to authenticate slow growing chicken meats. Their study was extended on a larger set and takes account of special labelling according to European regulation 91/1538/CEE. Our results show that the technique could be integrated in an analytical system of surveillance of slow-growing chicken meat products with quality mark

Novel Approach for Interlaboratory Transfer of Real-time PCR Methods: Detecting Bovine Meat and Bone Meal in Feed

The availability of robust methods for the species specific detection of meat and bone meal (MBM) in compound feedingstuffs, is an important prerequisite to enforce current and upcoming European legislation on the use of processed animal proteins in animal nutrition. Among possible methods, those based on DNA turned out to be a reliable tool for this aim, since DNA is a quite thermostable molecule able to resist severe heat treatments applied in the manufacturing of animal meals. The application of such methods by control laboratories implies that the method has been validated including an assessment of its robustness. Successful transferability between laboratories is considered an important robustness criterion of the method. However, corresponding guidelines regarding the design of such a study relevant to this field are missing. Here we demonstrate the feasibility of a new concept that was applied to check for the transferability of a qualitative assay for the detection of banned MBM in feedingstuffs at trace level based on real time PCR. This approach can be of interest for other research groups working in the development of real-time PCR methods and in their use by control laboratories.JRC.D.8-Food safety and qualit

Species and tissues specific differentiation of processed animal proteins in aquafeeds using proteomics tools

Significance: To guarantee the safe use of processed animal protein (PAP) in aquafeeds efficient PAP detection and monitoring tools are required. The present study investigated and compared various proteomics workflows and shows that the application of shotgun proteomics in combination with direct comparison of spectral libraries provides for the desired species and tissue specific classification of this heat sterilized and pressure treated (>= 133 degrees C, at 3 bar for 20 min) protein feed ingredient. (C) 2016 Elsevier B.V. All rights reserved.Proteomic

Detection of Ruminant Meat and Bone Meals in Animal Feed by Real-time Polymerase Chain Reaction (PCR): Results of an Interlaboratory Study

This article describes the main results from an interlaboratory study performed recently to determine the applicability of four different real-time PCR methods, developed by three National Expert laboratories from the European Union (EU), for the detection and identification of cattle or ruminant species in typical compound feeds, fortified with meat and bone meals (MBM) from different animal species at different concentration level and that followed the severe sterilisation condition mandatory within the European Union.JRC.D.8-Food safety and qualit

PCR Detection of Animal By-Products in Feeds (WP4)

In this article the authors present the development and validation of PCR based method for the detection of meat and bone meal (MBM) at various taxonomic levels. Special emphasis was placed on the impact of the sterilisation conditions on the performance characteristics of the PCR methodsJRC.D.8-Food safety and qualit