10 Years of C-K Theory: A Survey on the Academic and Industrial Impacts of a Design Theory.

The goal of our research1 was to understand what is expected today from a design theory and what types of impact such type of scientific proposition may reach. To answer these questions with a grounded approach we chosed to study the developement of C-K theory as phenomenon per se that can inform our research work. C-K theory is clearly recognized as a design theory and it is a good representative of the level of generality and abstraction of contemporary design theory. Indeed, the validity of the theory as such has already been documented (e.g. Hatchuel & Weil 2002, 2003, 2008, 2009; Kazakçi 2009; Reich et al 2010; Le Masson et al 2010; Ullah et al 2012). Instead the current work sets out to understand the dissemination and the impact of the theory in both academic and industrial fields. The data collection overlooks the literature on C-K theory in English and in French, and includes interviews and feedbacks of students and industrial partners who applied C-K methodologies and tools. This research confirms the rapid diffusion and multiples impact of C-K theory. Beyond, such study signals that there are important expectations and potential impacts of a Design Theory within the field of knowledge at large. However there are strong conditions to meet these expectations: generality, generativity, and relatedness to contemporary sciences. A similar research could be done on Nam Suh's axiomatic approach to further test these conditions. It is impossible to say what will be the next generations of Design theory but it is sure that they should progress on these directions

A survey on bacteria inhabiting the sea surface microlayer of coastal ecosystems

12 páginas, 5 tablas, 4 figurasBacterial populations inhabiting the sea surface microlayer from two contrasted Mediterranean coastal stations (polluted vs. oligotrophic) were examined by culturing and genetic fingerprinting methods and were compared with those of underlying waters (50 cm depth), for a period of two years. More than 30 samples were examined and 487 strains were isolated and screened. Proteobacteria were consistently more abundant in the collection from the pristine environment whereas Gram-positive bacteria (i.e., Actinobacteria and Firmicutes) were more abundant in the polluted site. Cythophaga-Flavobacter–Bacteroides (CFB) ranged from 8% to 16% of total strains. Overall, 22.5% of the strains showed a 16S rRNA gene sequence similarity only at the genus level with previously reported bacterial species and around 10.5% of the strains showed similarities in 16S rRNA sequence below 93% with reported species. The CFB group contained the highest proportion of unknown species, but these also included Alpha- and Gammaproteobacteria. Such low similarity values showed that we were able to culture new marine genera and possibly new families, indicating that the sea-surface layer is a poorly understood microbial environment and may represent a natural source of new microorganisms. Genetic fingerprinting showed, however, no consistent differences between the predominant bacterial assemblages from surface microlayer and underlying waters, suggesting that the presence of a stable and abundant neustonic bacterial community is not a common trait of coastal marine environments.This work was supported by the European Commission (Research Directorate General-Environment Program-Marine Ecosystems) through the AIRWIN project ‘‘Structure and role of biological communities involved in the transport and transformation of persistent pollutants at the marine AIR–Water Interface’’ (contract EVK3-CT2000-00030). The AIRWIN project is part of the EC IMPACTS cluster. During the final stage of this work EOC was supported by the Programa Ramo´n y Cajal from the Spanish MCYT.Peer reviewe

Comparison of samplers for the biological characterization of the sea surface microlayer

International audienceThe surface film of the hydrosphere covers more than 70% of the world’s surface. The sea surface microlayer(SML) or “skin” of the ocean is a sink for natural and anthropogenic material originating from the atmosphereand the water column. Organisms living in this SML are called “neuston.” Our knowledge of the biology of theSML is still in its infancy. Research of the sea surface microlayer requires the use of appropriate sampling techniques and strategies, and the question of what is the most suitable device has not yet been answered. In the present study, we have compared the efficiency of the Harvey glass plate (GP) and the Garrett metal screen (MS) to analyze a wide range of microbiological parameters in SML samples collected at two coastal stations in the NWMediterranean Sea. Two types of membranes (Teflon and polycarbonate) were also used to collect bacterioneuston. The MS was the most appropriate technique for most biological parameters providing higher enrichment factors as compared to the GP and, therefore, the highest enrichment factors compared with underlying waters (UW). Control experiments with UW demonstrated that the enrichment reported for the MS was not biased by any selectivity of the sampler itself. Therefore, we recommend the use of the MS when the aim is to compare different biological parameters. In contrast, there is clear evidence that hydrophobic and hydrophilic membranes have an important drawback and should not be used for quantification purposes

Design theory: a foundation of a new paradigm for design science and engineering

International audienceIn recent years, the works on Design Theory (and particularly the works of the Design Theory SIG of the Design Society) have contributed to reconstruct the science of design, comparable in its structure, foundations and impact to Decision Theory, Optimization or Game Theory in their time. These works have reconstructed historical roots and the evolution of design theory, conceptualized the field at a high level of generality and uncovered theoretical foundations, in particular the logic of generativity, the " design-oriented " structures of knowledge, and the logic of design spaces. These results give the academic field of engineering design an ecology of scientific objects and models, which allows for expanding the scope of engineering education and design courses. They have contributed to a paradigm shift in the organization of R&D departments, supporting the development of new methods and processes in innovation departments, and to establishing new models for development projects. Emerging from the field of engineering design, design theory development has now a growing impact in many disciplines and academic communities. The research community may play significant role in addressing contemporary challenges if it brings the insights and applicability of Design Theory to open new ways of thinking in the developing and developed world

Bacterioneuston Community Structure in the Southern Baltic Sea and Its Dependence on Meteorological Conditions▿†

The bacterial community in the sea surface microlayer (SML) (bacterioneuston) is exposed to unique physicochemical properties and stronger meteorological influences than the bacterial community in the underlying water (ULW) (bacterioplankton). Despite extensive research, however, the structuring factors of the bacterioneuston remain enigmatic. The aim of this study was to examine the effect of meteorological conditions on bacterioneuston and bacterioplankton community structures and to identify distinct, abundant, active bacterioneuston members. Nineteen bacterial assemblages from the SML and ULW of the southern Baltic Sea, sampled from 2006 to 2008, were compared. Single-strand conformation polymorphism (SSCP) fingerprints were analyzed to distinguish total (based on the 16S rRNA gene) and active (based on 16S rRNA) as well as nonattached and particle-attached bacterial assemblages. The nonattached communities of the SML and ULW were very similar overall (similarity: 47 to 99%; mean: 88%). As an exception, during low wind speeds and high radiation levels, the active bacterioneuston community increasingly differed from the active bacterioplankton community. In contrast, the particle-attached assemblages in the two compartments were generally less similar (similarity: 8 to 98%; mean: 62%), with a strong variability in the active communities that was solely related to wind speed. Both nonattached and particle-attached active members of the bacterioneuston, which were found exclusively in the SML, were related to environmental clones belonging to the Cyanobacteria, Bacteroidetes, and Alpha-, Beta-, and Gammaproteobacteria originally found in diverse habitats, but especially in water columns. These results suggest that bacterioneuston communities are strongly influenced by the ULW but that specific meteorological conditions favor the development of distinctive populations in the air-water interface

Vertical segregation and phylogenetic characterization of ammonia-oxidizing Archaea in a deep oligotrophic lake

12 páginas, 6 figuras, 1 tabla.Freshwater habitats have been identified as one of the largest reservoirs of archaeal genetic diversity, with specific lineages of ammonia-oxidizing archaea (AOA) populations different from soils and seas. The ecology and biology of lacustrine AOA is, however, poorly known. In the present study, vertical changes in archaeal abundance by CARD-FISH, quantitative PCR (qPCR) analyses and identity by clone libraries were correlated with environmental parameters in the deep glacial high-altitude Lake Redon. The lake is located in the central Spanish Pyrenees where atmospheric depositions are the main source of reactive nitrogen. Strong correlations were found between abundance of thaumarchaeotal 16S rRNA gene, archaeal amoA gene and nitrite concentrations, indicating an ammonium oxidation potential by these microorganisms. The bacterial amoA gene was not detected. Three depths with potential ammonia-oxidation activity were unveiled along the vertical gradient, (i) on the top of the lake in winter–spring (that is, the 0 oC slush layers above the ice-covered sheet), (ii) at the thermocline and (iii) the bottom waters in summer—autumn. Overall, up to 90% of the 16S rRNA gene sequences matched Thaumarchaeota, mostly from both the Marine Group (MG) 1.1a (Nitrosoarchaeum-like) and the sister clade SAGMGC−1 (Nitrosotalea-like). Clone-libraries analysis showed the two clades changed their relative abundances with water depth being higher in surface and lower in depth for SAGMGC−1 than for MG 1.1a, reflecting a vertical phylogenetic segregation. Overall, the relative abundance and recurrent appearance of SAGMGC−1 suggests a significant environmental role of this clade in alpine lakes. These results expand the set of ecological and thermal conditions where Thaumarchaeota are distributed, unveiling vertical positioning in the water column as a key factor to understand the ecology of different thaumarchaeotal clades in lacustrine environments.This research was supported by grants CRENYC CGL2006-12058 and PIRENA CGL2009-13318 to EOC, and CONSOLIDER grant GRACCIE CSD2007-00067 from the Spanish Office of Science and Innovation (MICINN). JCA benefits from a Juan de la Cierva postdoctoral fellow (MICINN).Peer reviewe