Determination of plasma vitellogenin levels and localization of vitellogenin in liver of Lake Van pearl mullet (Chalcalburnus tarichi Pallas, 1811)

This study focused on hepatosomatic and gonadosomatic index and levels of plasma 17β-estradiol (E2), vitellogenin (Vtg) and total protein and determined the localization of Vtg in the liver during vitellogenesis in female Chalcalburnus tarichi, an endemic cyprinid species living in the Lake Van, Turkey. The levels of plasma E2, Vtg and total protein in female fish increased during vitellogenesis and they are positively correlated with each other. According to the results from the female fish, the lowest levels of E2 and Vtg were measured in December at 120.60±23.38 pg ml^-1 and 1.10±0.26 mg ml^-1, respectively. The highest levels of plasma E2 and Vtg were in May (833.40±211.58 pg ml^-1) and April (31.31±4.9 mg ml^-1), respectively. The Vtg-positive hepatocytes were not uniform in liver of fishes. Generally, the hepatocytes surrounding the portal and central veins were strongly Vtg positive. These results showed that plasma Vtg levels in female fish increased during vitellogenesis related to plasma E2 and gonadosomatic index

Some macro and trace elements in various tissues of Van fish variations according to gender and weight

ABSTRACT Van fish is a fish that can live in soda water at pH 9-10. Van fishing area is a very important source of protein for the people. The variations in tissue metal levels according to gender and weight in Van fish (Alburnus tarichi, Güldenstadt 1814) were studied. This study was conducted for the first time in Van fish. Which it is very important for living organisms Calcium(Ca), magnesium(Mg), sodium(Na), potassium(K), cobalt(Co), chromium(Cr) and lithium(Li) elements levels in the muscle, liver, gill, gonad, and brain tissues of Van fish were investigated by using inductively-coupled plasma-optic emission spectroscopy (ICP-OES). The results were evaluated in two groups as macro-elements(Ca, Mg, Na, K) and trace-elements(Co, Cr, Li). Cobalt concentration in brain tissue was found to be higher than those in other tissues. Also, male fish had higher metal concentrations than female fish and their metal levels in brain and gonad tissues varied with their weight (P<0.05). This study shows that consumption of Van fish can be consumed to supply some necessary minerals required for a healthy nutrition

Some macro and trace elements in various tissues of Van fish variations according to gender and weight

<div><p>ABSTRACT Van fish is a fish that can live in soda water at pH 9-10. Van fishing area is a very important source of protein for the people. The variations in tissue metal levels according to gender and weight in Van fish (Alburnus tarichi, Güldenstadt 1814) were studied. This study was conducted for the first time in Van fish. Which it is very important for living organisms Calcium(Ca), magnesium(Mg), sodium(Na), potassium(K), cobalt(Co), chromium(Cr) and lithium(Li) elements levels in the muscle, liver, gill, gonad, and brain tissues of Van fish were investigated by using inductively-coupled plasma-optic emission spectroscopy (ICP-OES). The results were evaluated in two groups as macro-elements(Ca, Mg, Na, K) and trace-elements(Co, Cr, Li). Cobalt concentration in brain tissue was found to be higher than those in other tissues. Also, male fish had higher metal concentrations than female fish and their metal levels in brain and gonad tissues varied with their weight (P<0.05). This study shows that consumption of Van fish can be consumed to supply some necessary minerals required for a healthy nutrition.</p></div

Hematological and biochemical response in the blood of Alburnus tarichi (Actinopterygii: Cypriniformes: Cyprinidae) exposed to tebuconazole

Background. Lake Van, the second-largest soda lake in the world, has a pH 9.8 value. A fish, locally known as Van fish, Alburnus tarichi (Güldenstädt, 1814), is an endemic fish of Lake Van and known also as pearl mullet, tarek, or Van bleak. Tebuconazole is a widely used pesticide around Lake Van. In this study, we will focus on the effects of tebuconazole on Van fish blood to provide critical information on the environmental risk assessment of pesticides in various aquatic environments. Materials and methods. The Van fish were exposed to tebuconazole for 24, 48, 72, and 96 h at a concentration of 2.5 mg ·L–1. Subsequently, the resulting hematological and biochemical parameters were determined. Results. There was a statistically significant decrease in erythrocytes (RBC), hemoglobin (Hb), and hematocrit (Hct) in the blood parameters (P < 0.05). The levels of serum, aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), urea, and creatine increased significantly (P < 0.05). The serum cortisol level increased significantly at all hours after the administration of tebuconazole (P < 0.05). Conclusion. As a result, toxicity caused by pesticides caused negative changes in the biochemical and hematological values of Van fish. Changes in these parameters have shown that it can be used as a biomarker for toxicity

Heat shock protein 70 expression in neonatal rats after hypoxic stress

Objectives: The tissue damage due to hypoxia in newborns is to some-extent age-dependent; organs of premature babies are more vulnerable to hypoxic insult than full-term neonates. The aim of this immunohistochemical study was to investigate the role of heat shock protein 70 (HSP70), a stress-inducible protein, in developing the response to hypoxia in premature newborns. Methods: Postnatal day-7 rats (corresponding to a human fetus of 32-34 weeks' gestation) and day-12 rats (corresponding to a full-term newborn infant) (n = 7) were subjected to mild hypoxia at 33°C. Control rats (n = 7) for each group breathed room air for 4 h. After 4 h of recovery, the animals were killed, and brains, hearts and kidneys were removed for immunohistochemical staining. Results: Immunohistochemically, HSP70 expression was found to be induced in the hippocampus and myocardium after exposure to hypoxia. The level of HSP70 expression in the hippocampus after hypoxic stress was significantly higher in the 12-day rats than in the 7-day rats (p = 0.03). However, HSP70 expression in the myocardium did not show any significant difference between the two groups. In addition, no significant induction of HSP70 expression was apparent in the kidney of rats exposed to hypoxia or in any organ of the control animals. Conclusions: We conclude that diminished HSP70 expression in the hippocampus of premature newborns may play a critical role in developing the response to hypoxic stress. However, HSP70 expression in the heart and the kidney after exposure to hypoxia did not appear to be related to fetal maturity