1,662 research outputs found

    Time resolved spectroscopy of the multiperiodic pulsating subdwarf B star PG1605+072

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    We present results for the 2m spectroscopic part of the MultiSite Spectroscopic Telescope campaign, which took place in May/June 2002. In order to perform an asteroseismological analysis on the multiperiodic pulsating subdwarf B star PG 1605+072 we used over 150 hours of time resolved spectroscopy in order to search for and analyse line profile variations by using phase binning. We succeeded in finding variations in effective temperature and gravity for four modes. A pilot analysis using the \textit{BRUCE} and \textit{KYLIE} programs and assuming strong rotation and low inclination favours models with l=1l=1 or l=2l=2 with m≤0m\leq0.Comment: 2 pages, 2 figures, proceedings of the "Vienna Workshop on the Future of Asteroseismology", to appear in Communications in Asteroseismology v. 14

    Mode identification for Balloon 090100001 using combined multicolour photometry and spectroscopy

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    In this paper, we show that method of mode identification using combined multicolour photometry and spectroscopy can be successfully applied to the pulsating subdwarf B star Balloon 090100001. The method constrains the spherical degree, l.We confirm that the dominant mode is radial and we show that for some other modes the method provides values of l consistent with the observed rotationally split triplet. Moreover, we derive a radius variation of 1.7 per cent for the dominant mode. The identification opens the possibility for constraining the internal structure of the star by means of seismic methods.Comment: MNRAS, in press, already at the online-early stag

    The subdwarf B star SB 290 - A fast rotator on the extreme horizontal branch

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    Hot subdwarf B stars (sdBs) are evolved core helium-burning stars with very thin hydrogen envelopes. In order to form an sdB, the progenitor has to lose almost all of its hydrogen envelope right at the tip of the red giant branch. In close binary systems, mass transfer to the companion provides the extraordinary mass loss required for their formation. However, apparently single sdBs exist as well and their formation is unclear since decades. The merger of helium white dwarfs leading to an ignition of core helium-burning or the merger of a helium core and a low mass star during the common envelope phase have been proposed. Here we report the discovery of SB 290 as the first apparently single fast rotating sdB star located on the extreme horizontal branch indicating that those stars may form from mergers.Comment: 5 pages, 4 figures, A&A letters, accepte

    Non-invasive and non-destructive measurements of confluence in cultured adherent cell lines

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    Many protocols used for measuring the growth of adherent monolayer cells in vitro are invasive, destructive and do not allow for the continued, undisturbed growth of cells within flasks. Protocols often use indirect methods for measuring proliferation. Microscopy techniques can analyse cell proliferation in a non-invasive or non-destructive manner but often use expensive equipment and software algorithms. In this method images of cells within flasks are captured by photographing under a standard inverted phase contract light microscope using a digital camera with a camera lens adaptor. Images are analysed for confluence using ImageJ freeware resulting in a measure of confluence known as an Area Fraction (AF) output. An example of the AF method in use on OVCAR8 and UPN251 cell lines is included. •Measurements of confluence from growing adherent cell lines in cell culture flasks is obtained in a non-invasive, non-destructive, label-free manner. •The technique is quick, affordable and eliminates sample manipulation. •The technique provides an objective, consistent measure of when cells reach confluence and is highly correlated to manual counting with a haemocytometer. The average correlation co-efficient from a Spearman correlation (n = 3) was 0.99 ± 0.008 for OVCAR8 (p = 0.01) and 0.99 ± 0.01 for UPN251 (p = 0.01) cell lines

    Carboplatin and taxol resistance develops more rapidly in functional BRCA1 compared to dysfunctional BRCA1 ovarian cancer cells

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    A major risk factor for ovarian cancer is germline mutations of BRCA1/2. It has been found that (80%) of cellular models with acquired platinum or taxane resistance display an inverse resistance relationship, that is collateral sensitivity to the other agent. We used a clinically relevant comparative selection strategy to develop novel chemoresistant cell lines which aim to investigate the mechanisms of resistance that arise from different exposures of carboplatin and taxol on cells having BRCA1 function (UPN251) or dysfunction (OVCAR8). Resistance to carboplatin and taxol developed quicker and more stably in UPN251 (BRCA1-wildtype) compared to OVCAR8 (BRCA1-methylated). Alternating carboplatin and taxol treatment delayed but did not prevent resistance development when compared to single-agent administration. Interestingly, the sequence of drug exposure influenced the resistance mechanism produced. UPN251-6CALT (carboplatin first) and UPN251-6TALT (taxol first) have different profiles of cross resistance. UPN251-6CALT displays significant resistance to CuSO4 (2.3-fold, p=0.004) while UPN251-6TALT shows significant sensitivity to oxaliplatin (0.6-fold, p=0.01). P-glycoprotein is the main mechanism of taxol resistance found in the UPN251 taxane-resistant sublines. UPN251 cells increase cellular glutathione levels (3.0-fold, p=0.02) in response to carboplatin treatment. However, increased glutathione is not maintained in the carboplatin-resistant sublines. UPN251-7C and UPN251-6CALT are low-level resistant to CuSO4 suggesting alterations in copper metabolism. However, none of the UPN251 sublines have alterations in the protein expression of ATP7A or CTR1. The protein expression of BRCA1 and MRP2 is unchanged in the UPN251 sublines. The UPN251 sublines remain sensitive to parp inhibitors veliparib and CEP8983 suggesting that these agents are candidates for the treatment of platinum/taxane resistant ovarian cancer patients

    The MultiSite Spectroscopic Telescope campaign: 2m spectroscopy of the V361 Hya variable PG1605+072

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    We present results and analysis for the 2m spectroscopic part of the MultiSite Spectroscopic Telescope (MSST) campaign undertaken in May/June 2002. The goal of the project was to observe the pulsating subdwarf B star PG1605+072 simultaneously in velocity and photometry and to resolve as many of the >50 known modes as possible, which will allow a detailed asteroseismological analysis. We have obtained over 150 hours of spectroscopy, leading to an unprecedented noise level of only 207m/s. We report here the detection of 20 frequencies in velocity, with two more likely just below our detection threshold. In particular, we detect 6 linear combinations, making PG1605+072 only the second star known to show such frequencies in velocity. We investigate the phases of these combinations and their parent modes and find relationships between them that cannot be easily understood based on current theory. These observations, when combined with our simultaneous photometry, should allow asteroseismology of this most complicated of sdB pulsators.Comment: 9 pages, 5 figures, accepted for publication in A&A; Figure 1 at lower resolution than accepted versio

    Abundance studies of sdB stars using UV echelle HST/STIS spectroscopy

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    Aims: We test the hypothesis that the pulsations in sdB stars are correlated with the surface abundances of iron-group elements. Any correlation might explain why, when given two spectroscopically similar stars, one will pulsate while the other will not. Methods: We have obtained high-resolution ultraviolet spectra two pulsating and three non-pulsating sdB stars using the Space Telescope Imaging Spectrograph onboard the Hubble Space Telescope. We determined abundances for 25 elements including the iron group and even heavier elements such as tin and lead using LTE curve-of-growth and spectrum synthesis techniques. Results: We find no clear correlation between pulsations and metal abundances, and we comment on the resulting implications, including whether it is possible to determine the difference between a pulsating and a non-pulsating sdB spectroscopically. In addition to the main goal of our observations, we have also investigated the effect of supersolar metallicity on fundamental parameter determination, possible trends with iron abundance, and the hypothesis that weak winds may be selectively removing elements from the stellar envelopes. These effects provide challenges to stellar atmosphere modelling and diffusion models for sdB stars.Comment: 14 pages, 12 figures, accepted for publication in A&
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