Surveying viruses on ornamental trees and shrubs in two Hungarian botanical gardens and an arboretum

In Hungary the most common disease of fruit trees causing the most severe damages is Plum pox virus (PPV). This is why it is important to know other woody host plants that can be considered as source of infection. National surveys have been carried out since 2002 for revealing the distribution of PPV on ornamental and wild Prunus species.From 2005 this work has been extended to studying other viruses on other woody plants. In two botanical gardens and an arboretum we selected plants (species and varieties) showing virus symptoms among various thousands of woody plants and submitted them to indexing on woody and herbaceous indicators, as well as to testing with ELISA for the presence of the following 11 viruses occurring on woody plants: PPV, Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV), Cherry leafroll virus (CLRV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple mosaic virus (ApMV), Apple chlorotic leafspot virus (ACLSV), Strawberry latent ringspot virus (SLRV), Tomato black ring virus (TBRV) and Arabis mosaic virus (ArMV).Up to now, in 28 plant species and varieties, PPV, PDV, PNRSV, CLRV and ASPV have been detected so far. The presence of PPV was found in 9 species/varieties, such as Prunus cerasifera ’Pendula’, P. cerasifera ’Pissardii’, P. glandulosa, P. glandulosa ‘Alba Plena’, P. glandulosa ’Sinensis’, P. japonica, P. sogdiana, P. tomentosa (from Tibet) and P. x blireana.Seventeen species/varieties were found to be infected with PDV: Lonicera caucasica, L. maackii, L. sachalinensis, Prunus mume ’Omoi-no-mama’, P. salicina (from China), P. spinosa ’Plena’, P. spinosa ’Purpurea’, P. serrulata ’Amanogawa’, P. serrulata ’Ichiyo’, P. serrulata ’Pink Perfection’, P. serrulata ’Taihaku’, P. serrulata ’Yedo-sakura’, P. subhirtella ’Plena’, P. tenella, P. yedoensis, P. yedoensis ’Moerheimii’ and Syringa yunnanensis. Certain species/varieties infected with PDV were positive also for CLRV, such as P. spinosa ’Purpurea’, P. yedoensis, P. yedoensis ’Moerheimii’, P. subhirtella ’Plena’, P. serrulata ’Yedo-sakura’ and to ASPV: P. subhirtella ’Plena’ and P. serrulata ’Yedo-sakura’. PNRSV was detected in P. cerasifera ’ Nigra ’ and ’Hollywood’. The difference of symptoms provoked by PDV on herbaceous hosts suggests that various strains of this virus affect hosts, mainly yellow mottle disease described as a separate strain by Ramaswamy and Posnette on ornamental cherries in 1972. Molecular studies are in progress to confirm the above results. Keywords: ornamental trees and shrubs, virus symptoms, ELISA, Prunus, Lonicera, Syring

Comparison of Raspberry bushy dwarf virus isolates from Hungary and Slovenia

In 2006 and 2007 samples of grapevine and Rubus species were collected and analysed by DAS-ELISA to survey the presence of Raspberry bushy dwarf virus (RBDV) in Slovenia and Hungary. Seven varieties of raspberry from one Hungarian collection orchard were found to be infected. In Slovenia the presence of RBDV was confirmed only in three samples of wild Rubus. None of the 133 samples from different locations in Hungary proved to be infected with RBDV, although this virus is found to be widely distributed in grapevine in neighbouring Slovenia. Serological characterisation with three monoclonal antibodies (R2, R5 and D1) was performed on positive samples. Selected positive samples were partially sequenced. The results of serological and molecular analyses were compared with the analyses of raspberry and grapevine isolates obtained in Slovenia from other projects and published RBDV sequences from the GeneBank database to study the variability among hosts and locations. Isolates from grapevine grouped separately from the black raspberry isolate and all the red raspberry isolates. RBDV isolates from Hungarian samples formed a subgroup within red and black raspberry group.Keywords: RBDV, variability, Rubus, raspberry, grapevine, sequences, monoclonal antibodie

From taxonomies to ontologies: formalizing generalization knowledge for on-demand mapping

© 2015 Cartography and Geographic Information Society Automation of the cartographic design process is central to the delivery of bespoke maps via the web. In this paper, ontological modeling is used to explicitly represent and articulate the knowledge used in this decision-making process. A use case focuses on the visualization of road traffic accident data as a way of illustrating how ontologies provide a framework by which salient and contextual information can be integrated in a meaningful manner. Such systems are in anticipation of web-based services in which the user knows what they need, but do not have the cartographic ability to get what they want

The Alsónyék story: towards the history of a persistent place

No abstract available

Longhouse times: dating the Alsónyék LBK settlement

No abstract available