ASSAY FOR AFLATOXINS IN SOME LOCAL FOOD CONDIMENTS

Aflatoxins are secondary metabolites with toxicological properties that induce a variety of health challenges when foods contaminated with these compounds are ingested. Aflatoxins are stable under most food processing conditions and therefore persist to the final products. Fresh samples of thirty two food condiments comprising of four each of Pleurotus tuber regium (Osu), Piper guineense (Uziza), Xylopia aethiopica (Uda), Monodora mystrica (Ehuru), Citrullus vulgaris (fermented mellon), Ricinus communis (Ogiri) Brachystegia eurycoma (Achi) and Origanum syriacum (Offor) were assayed for fungi and presence of aflatoxins. The mean fungal counts range from 1.2 x 104 in Pleurotus tuber regium to 8.2 x 109 in Brachystegia eurycoma. Eight fungal spp were identified to include Aspergillus, Penicillium, Candida, Mucor, Rhizopus, Geotricum, Saccharomyces and Fusarium. Aflatoxin assay by RIDA quick aflatoxin kit detected two samples of Brachystegia eurycoma and a sample of Origanum syriacum with concentrations ≥ 20ppb. The best protection against mycotoxins is monitoring their presence in foods and feeds, therefore, regular and routine analysis of food samples for possible presence of mycotoxins is recommended. Education and training of food handlers is imperative, adoption of GMP and HACCP application in food condiment preparation will curtail fungal contamination and mycotoxin production in foods and feed product

In vivo antimalarial effect of Ananas comosus (L) Merr (Bromeliaceae) fruit peel, and gas chromatography-mass spectroscopy profiling: A possible role for polyunsaturated fatty acid

Purpose: To evaluate the antimalarial effect of Ananas comosus extract and fractions and also to identify the likely bioactive compounds.Method: The fruit peel of the plant was extracted with methanol, and the extract successively fractionated with n-hexane, dichloromethane, ethyl acetate, methanol and water. The n-hexane fraction was further subjected to vacuum liquid chromatography to afford four sub-fractions, one of which was also analyzed using gas chromatography-mass spectroscopy (GC-MS). Plasmodium berghei-infected mice were treated orally with three doses (100, 200 and 400 mg/kg) of the plant extract and a single dose (200 mg/kg) of each of the fractions and sub-fractions in a curative antimalarial model using artemisinin combination therapy (ACT) as the reference drug.Results: The extract exhibited significant (p < 0.001) non-dose dependent parasitemia inhibitory activity in the range of 44.84 to 76.09 %. All fractions displayed inhibitory effect (p < 0.001) in the range of 46.44 to 87.58 % with the dichloromethane fraction displaying the highest effect (87.58 %). The subfractions exhibited significant inhibitory effect (p < 0.001) in the range of 84.14 to 92.54 %. The ACT produced significant (p < 0.001) inhibitory effect of 83.92 %. GC-MS analysis revealed the presence of 17 bioactive compounds, the most abundant of which were linoleic acid and palmitic acid.Conclusion: A. comosus displays strong antimalarial activity which supports the folkloric use of the plant for malarial treatment. A polyunsaturated fatty acid (linoleic acid) was the most abundant phytoconstituent identified. Keywords: Ananas comosus, Antimalarial, Malaria, Pineapple, Plasmodium beighe

In vivo evaluation of the antiviral activity of Cajanus cajan on measles virus

Cajanus cajan, a tropical shrub, serves as source of food and traditional medicines. The evaluation of aqueous and ethanol extracts for activity against measles virus and toxicity to embryonated chicken eggs was carried out in this study. In vivo and in vitro assay techniques using embryonated chicken eggs and tissue culture (Hep-2 cell lines) as media for both virus cultivation and anti-virus assay showed that a hot-water extract yielded higher activity against measles virus. The hot-water extract of the stem yielded a Log2 titre of 0.1 for the in vivo assay and an inhibition of cytopathic effect (CPE) in Hep-2 cells by 100% for the in vitro assay. At all concentrations of the extracts, there was a lowering of virus concentration (p = 0.05), indicated by hemagglutination (HA) titration, which is the advantage of HA titration over the tissue culture technique using CPE. This study validates embryonated chicken eggs as suitable media for anti-virus assay and the use of C. cajan in the treatment of some diseases of viral origin

Distribution of Vibrio species in Shellfish and Water Samples Collected from the Atlantic Coastline of South-East Nigeria

Crayfish, lobster, and sea-water samples collected from five fishing islands on the Atlantic coast\u2013Bight of Biafra (Bonny)\u2013belonging to Ibaka Local Government Area of Akwa-Ibom State of Nigeria were bacteriologically evaluated on thiosulphate citrate bile-salt sucrose (TCBS) agar for Vibrio load and pathotypes. Mean log10 Vibrio counts of 7.64\ub12.78 cfu/g (in crayfish), 5.07\ub13.21 cfu/g (in lobster), and 3.06\ub12.27 cfu/mL (in sea-water) were obtained in rainy season (June-July) while counts in the dry season (November-December) were 6.25\ub11.93 cfu/g, 5.99\ub11.54 cfu/g, and 3.84\ub11.78 cfu/mL respectively. The physicochemical measurements (temperature, pH, and total dissolved solutes) of the sea-water did not vary significantly in the two seasons across all five islands. Vibrio species isolated were Vibrio cholerae (both O1 and non-O1 serotypes), V. parahaemolyticus , V. vulnificus , V. mimicus , and V. fluvialis . Both Ogawa and Inaba subtypes of V. cholerae O1 serotype were found. In addition, the Hikojima subtype, which had not been previously reported in the region, was isolated in two samples. The results show that these Vibrio species are endemic in the area

Genetic characterization of non-O1/non-O139 <i>Vibrio cholerae</i> mobilome: a strategy for understanding and discriminating emerging environmental bacterial strains

Acute diarrhea and cholera (AWD/C) result in more than 21000 to 143000 global mortality annually and are associated with Vibrio cholerae. The pathogen has shown increasing evolutionary/emerging dynamics linked with mobilome or ubiquitous nature of mobile integrative genetic and conjugative elements (MIGCE), however, such dynamics are rarely reported amongst somatic-antigen non-agglutinating Type-1/-139 V. cholerae (SA-NAG-T-1/139Vc). The study reports the genetic detection of mobilome-associated indices in SA-NAG-T-1/139Vc as a potential strategy for differentiating/discriminating emerging environmental bacteria. Presumptive V. cholerae isolates were retrieved from five water sources, while strains were characterized/serogrouped and confirmed using simplex and comparative-genomic-multiplex Polymerase Chain Reaction (PCR). Genomic island (GI-12det, GI-14det, GI-15det); Phages (TLC-phagedet, Kappa-phagedet) and ICEs of the SXT/R391 family genes (SXT/R391-ICEs integrase, SXT-Hotspot-IV, ICEVchInd5Hotspot-IV, ICEVchMoz10Hotspot-IV) were detected. Other rare ICE members such as the ICEVcBan8att gene and Vibrio Seventh Pandemic island detection (VSP-II Integrase, Prototypical VSP-II) were also detected. Results revealed that the 8.22% (61/742) SA-NAG-T-1/139Vc serogroup observed harbors the Vibrio Seventh Pandemic island integrase (34/61; 55.7%) and other rare genetic traits including; attB/attP (29/61; 47.5%, 14/61; 23%), integrative genetic elements (4/61; 6.56%), phage types (TLC-phagedet: 2/61; 3.28% and Kappa-phagedet: 7/61; 11.48%) as well as the integrase genes (INT1, Sul1, Sul2) (29/61: 47.5%; 21/61: 34.4%; 25/61: 41%). Such genetic detection of mobilome determinants/MIGCE suggests potential discriminatory tendencies amongst SA-NAG-T-1/139Vcwhich may be applied in mobilome typing of evolving/emerging environmental bacteria. The need to encourage the application of such mobilome typing indices and continuous study of these strains is suggestive of interest in controlling future potential emerging environmental strains

Investigation of the chemical composition and biological activity of Xylopia aethiopica Dunal (Annonacae)

The phytochemical composition and physicochemical properties of oil extractable from the fruits of Xylopia aethiopica were determined. Extracts’ effects on cell membrane stability and prostaglandin synthetase activity were also evaluated. X. aethiopica oil extracted with chloroform: methanol (2:1, v/v) mixture contained carbohydrates, glycosides, flavonoids, saponins, tannins and phytosterols. The characteristic volatile and sweet smelling nature of X. aethiopica was predominantly inherent in this fraction. The sterol content was 64.30 mg/100 ml; with a high degree of unsaturatedness as evident in its high iodine value (85.76). High pressure liquid chromatographic analysis of the lipid extract revealed a fatty acid profile of palmitic acid (19.21%), palmitoleic acid (0.81%), stearic acid (4.54%), oleic acid (39.12%), linoleic acid (25.98%) and linolenic acid (1.10%). Investigation of the effect of the extract on hypotonicity- induced haemolysis of human red blood cells produced by water showed that the methanol extract of X. aethiopica (XAME) stabilized the red blood cells against the haemolytic action of distilled water. The lipid extract, on the other hand did not show any protective action against the osmotic shock. Xylopia aethiopica fruits may therefore be helpful in the maintenance of the integrity of the cellular membranes. The lipid extract also, in vitro, exhibited a prostaglandin synthetase substrate activity, whereas the methanol extract enhanced the synthesis of prostaglandins using X. aethiopica oil as  substrate. The presence of appreciable quantity of unsaturated fatty acids, stabilization of the cellular membrane integrity, promotion of the  biosynthesis of the hormone-like substances, prostaglandins, may be responsible for the usefulness of X. aethiopica fruits in the healing of wounds, inflammatory disorders and treatment of post-natal pains.Key words: Xylopia, linoleic acid, membranes, haemolysis, pains

Bioflocculant production by a consortium of Streptomyces and Cellulomonas species and media optimization via surface response model

AbstractSpecies of actinobacteria previously isolated from Tyume River in the Eastern Cape Province of South Africa and identified by 16S rDNA sequence as Cellulomonas and Streptomyces species were evaluated as a consortium for the production of bioflocculant. Sucrose, peptone and magnesium chloride were the nutritional sources which supported optimal production of bioflocculant resulting in flocculation activities of 91%, 82% and 78% respectively. Response surface design revealed sucrose, peptone and magnesium chloride as critical media components following Plackett–Burman design, while the central composite design showed optimum concentration of the critical nutritional source as 16.0g/L (sucrose), 1.5g/L (peptone) and 1.6g/L (magnesium chloride) yielding optimal flocculation activity of 98.9% and bioflocculant yield of 4.45g/L. FTIR spectrometry of the bioflocculant indicated the presence of carboxyl, hydroxyl and amino groups, typical for heteropolysaccharide, while SEM imaging revealed an interwoven clump-like structure. The molecular weight distribution of the constituents of the bioflocculants ranged 494.81–18,300.26Da thus, an indication of heterogeneity in composition. Additionally, the chemical analyses of the purified bioflocculant revealed the presence of polysaccharides and proteins with neutral sugar, amino sugar and uronic acids in the following concentration: 5.7mg, 9.3mg and 17.8mg per 100mg. The high flocculation activity of the bioflocculant suggests commercial potential

Health Implications of Occupational Exposure of Butchers to Emissions from Burning Tyres

Background: Flames from burning scrap tyres are used in de-furring animals for human consumption in most parts of Nigeria. Emissions from tyres are known to contain a myriad of toxic mixtures especially particulate matter (PM), volatile organic compounds, hazardous air pollutants, and inspirable metals, some of which are known human carcinogens. This cross-sectional study investigated the deleterious health effects of these emissions in occupationally-exposed workers at the Dei-Dei Abattoir, Abuja, Nigeria. Methods: A total of 156 respondents were divided into two groups. Group 1 (124 butchers) and group 2 [32 administrative staff (AS)]. Data from digital spirometry were used to determine the association between chronic exposure to tyre emissions and lung function. Urinary 1-Hydroxypyrene concentration, phenolic compounds levels and heavy metal concentrations were determined. Also ambient PM and polycyclic aromatic hydrocarbons (PAHs) concentrations at 3 delineated points in the abattoir were measured. Findings: Spirometry results showed significant deterioration of lung function in the butchers. The concentration of 1-Hydroxypyrene (μg/molCret) in the post-shift urine samples of the butchers was significantly higher (P < 0.05) in butchers relative to the AS (0.52 ± 0.13 Vs 0.20 ± 0.07, respectively). Similarly the concentrations of zinc and nickel (mg/l) were significantly higher in the butchers compared to the AS (zinc: 0.91 ± 0.19 Vs 0.31 ± 0.28, respectively; nickel: 0.11 ± 0.06 Vs 0.06 ± 0.02, respectively). Anthracene, fluoranthene, pyrene, benzo-a- pyrene, and PM concentrations were significantly higher at the de-furring point when compared to the wash bay and the administrative building, especially between 8.00 and 8.30 am. Conclusion: Occupational exposure to scrap tyre emissions resulted in significant adverse health effects. The existing laws banning the use of burning tyres in meat processing should be enforced while the use of personal protective equipment should be encouraged in abattoirs

Assessment of background ionizing radiation exposure levels in industrial buildings in Nnewi, Anambra State, Nigeria

Background: Increased exposure from background radiations and the attendant health effects have in recent times drawn the attention of researchers. This study aimed to assess the indoor and outdoor background radiation levels in selected offices/industrial buildings in Nnewi, Anambra State, Nigeria. Methods: Forty buildings in the four villages of Nnewi were surveyed using a calibrated international medicom CRM 100 radiation monitor. Radiation readings were obtained in counts per minute and converted to micro-sieverts per hour (µSv h-1). The indoor annual effective dose rate (IAEDR), outdoor annual effective dose rate, excess lifetime cancer risk, and organ doses were calculated using recommended occupancy and conversion factors.Results: The mean IAEDR and OAEDR were respectively 0.8060±0.056 mSv y-1 and 0.2281±0.020 mSv y-1 with estimated ELCR of 2.822x10-3 and 0. 799x10-3 respectively. The testes received the highest dose (0.843 mSv y-1) followed by bone marrow (0.710 mSv y-1).Conclusions: The study revealed that the mean background radiation exposures in and outside offices in Nnewi, Anambra State were below the UNSCEAR and ICRP recommended doses for the general public

The AU free movement protocol: Challenges in its implementation

One of the major pillars of the African Union is the integration of peoples and the ability for them to move freely from one member country to another, with the right to reside and practise their trade or profession. This aspect of integration found full expression in the Protocol to the Treaty Establishing the African Economic Community Relating to the Free Movement of Persons, Right of Residence andRight of Establishment, adopted in 2018. Upon operationalization, it will remove obstacles to the movement of people, capital and resources in the region and give expression to aspiration 2 of the African Union Agenda 2063. However, significant challenges lie on the path of its implementation. This article doctrinally reviews the protocol, looking at its prospects for promoting African integration and development, anticipates some of the problems that the protocol will face. It concludes with recommendations for achieving its lofty but desirable ends