Identifikasi Molekuler Bakteri Staphylococcus sp. dan Staphylococcus aureus Penyebab Mastitis Subklinis pada Ternak Kambing Perah

Mastitis merupakan radang pada glandula mammae (ambing) ternak perah. Mastitis tipe subklinis sering dikaitkan pada kejadian mastitis di peternakan ruminansia kecil seperti kambing perah (kambing Peranakan Etawah, Saanen, dan Sapera). Patogen utama yang berperan dalam kejadian mastitis ini adalah genus Staphylococcus. Tujuan penelitian adalah untuk melakukan identifikasi bakteri Staphylococcus sp. dan Staphylococcus aureus sebagai penyebab mastitis subklinis pada kambing perah dengan menggunakan metode polymerase  chain reaction (PCR). Tahapan metode yang dilakukan adalah ekstraksi DNA dengan teknik spin-collumn system terhadap 26 isolat bakteri yang telah dilakukan uji biokimia sebelumnya dan amplifikasi gen spesifik 23s rRNA Staphylococcus sp. dan Staphylococcus aureus, serta methicillin resistant Staphylococcus aureus (MRSA), dilanjutkan dengan visualisasi menggunakan UV-transluminator. Hasil menunjukkan bahwa sebanyak 12 isolat sampel teridentifikasi Staphylococcus sp. dan 1 diantaranya teridentifikasi Staphylococcus aureus. Isolat yang teridentifikasi Staphylococcus aureus bukan termasuk MRSA.

Potensi Bentonite Clay Dan Karbon Aktif Sebagai Aflatoksin Binders Berdasarkan Gambaran Imunologis Dan Histopatologis Pada Ayam Buras

Aflatoxin is a toxic compound from the fungus A. flavus which can cause a decrease in chicken productivity. This study aims to evaluate the effect of bentonite clay and activated charcoal in chicken feed as aflatoxin binders based on histopathological and immunological features in chickens. A total of 60 chickens were randomly divided into 4 groups (G0, G1, G2, and G3) each group consisted of 15 chickens. G0 as a control without the addition of aflatoxin to the feed. Groups G1, G2, and G3’s feed were added with 106 ppb of aflatoxin. G2 was treated with 5g/kg bentonite clay, G3 was treated with 5g/kg activated charcoal. Chickens were vaccinated with Newcastle Disease (ND) on day 5. On days 14 and 28 serum was collected from the specimens for titer antibody testing. At the end of the study the chickens were dissected, the liver was taken for a histopathological test. The results of the aflatoxin level in the G0, G1, G2, and G3 groups were 10.5, 106, 19.1, and 23.95 ppb, respectively. Microscopic changes show the negative effects of aflatoxin on the liver organ. Group G0 did not show any histopathological changes, Group G2 and G3 showed several mild histopathological changes, whereas group G1 was the most severe. The antibody titers against Newcastle Disease (ND) of 4-weeks old chickens in G0, G1, G2, and G3 were 64, 4, 32, 32 HI units, respectively. The G0 treatment group has a protective titer against ND disease. Group G1 has the lowest (non-protective) ND antibody titer. Whereas the G2 and G3 groups both have protective titers against ND disease. Based on this study it was concluded that the administration of bentonite clay and activated charcoal was able to produce better immunological and histopathological features than the control group

Potential of Dairy Cattle Development in Boyolali Central Java, Indonesia

This study is one part of the evaluation and policy research of dairy cattle development in Boyolali, Central Java. This studyaimed: 1) to analyze demographic of farmers characteristics, 2) to recover the potential of availability of land forage and water availability , 3) to figure out thetrend ofdairy cowpopulationandmilkproduction. This research was carried out from December 2010 to March 2011 at three of village cooperative units, consists of Mojosongo cooperative, Musuk cooperative, and Cepogo cooperative. This research using survey method. The number of respondents used in this research was 266 farmers. Primary data were collected by interviews based on questionnaires. Secondary data were collected from the government related with this research. The data were analyzed by multiple linier regression analysis. The results showed that the farmer s characteristic influenced to the milk production. R2 value for 0, 549, it showed that 54,9% of milk production can be explained by the independent variables, and 45,1?n be explained by other variables beyond the model. Availability of land forage and water availability influenced to the milk production significantly (P<0.001). 40,2% of milk production can be explained by the availability of land forage and water. The results also showed that the dairy cow population was significantly positive effect (P <0.01) on milk production. The prediction results showed that dairy cow population and milk production of dairy cows in the 10 years later would increase

Effect of Farmers' Characteristics, Forage Land and Water Availability for Dairy Milk Production in Boyolali, Central Java, Indonesia

This study is one part of the research potential of the dairy cattle development in Central Java Boyolali, Indonesia. This study aims to analyze the influence of the characteristics of dairy farmers, forage land and water availability on dairy cow milk production in Central Java Boyolali, Indonesia. This research was conducted from March 2010 until January 2011 in three dairy cooperatives: Mojosongo cooperatives, Musuk cooperative, and Cepogo cooperatives using the survey method. Total respondents used in this study were 266 farmers divided into three stratum. Primary data was collected through interviews based on a questionnaire that has been prepared. Data were analyzed using multiple linear analysis simple. The analysis showed that characteristics of dairy farmers, forage land and water availability together significantly influenced the milk production. At stratum R2 value of 0.965 suggesting that milk production could be explained by factors independent as much as 96.50 %, while the remaining 3.50 % is caused by other factors outside the model. At stratum II R2 value of 0.635 suggesting that milk production could be explained by factors independent as much as 63.50 %, while the remaining 35.50 % is caused by other factors outside the model. At stratum III R2 value of 0.681 suggesting that milk production could be explained by factors independent as much as 68.10 %, while the remaining 31.90 % is caused by other factors outside the model

Effect of Farmers’ Characteristics, Forage Land and Water Availability for Dairy Milk Production in Boyolali, Central Java, Indonesia

This study is one part of the research potential of the dairy cattle development in Central Java Boyolali, Indonesia. This study aims to analyze the influence of the characteristics of dairy farmers, forage land and water availability on dairy cow milk production in Central Java Boyolali, Indonesia. This research was conducted from March 2010 until January 2011 in three dairy cooperatives: Mojosongo cooperatives, Musuk cooperative, and Cepogo cooperatives using the survey method. Total respondents used in this study were 266 farmers divided into three stratum. Primary data was collected through interviews based on a questionnaire that has been prepared. Data were analyzed using multiple linear analysis simple. The analysis showed that characteristics of dairy farmers, forage land and water availability together significantly influenced the milk production. At stratum R2 value of 0.965 suggesting that milk production could be explained by factors independent as much as 96.50 %, while the remaining 3.50 % is caused by other factors outside the model. At stratum II R2 value of 0.635 suggesting that milk production could be explained by factors independent as much as 63.50 %, while the remaining 35.50 % is caused by other factors outside the model. At stratum III R2 value of 0.681 suggesting that milk production could be explained by factors independent as much as 68.10 %, while the remaining 31.90 % is caused by other factors outside the model

Effect of Farmers’ Characteristics, Forage Land and Water Availability for Dairy Milk Production in Boyolali, Central Java, Indonesia

This study is one part of the research potential of the dairy cattle development in Central Java Boyolali, Indonesia. This study aims to analyze the influence of the characteristics of dairy farmers, forage land and water availability on dairy cow milk production in Central Java Boyolali, Indonesia. This research was conducted from March 2010 until January 2011 in three dairy cooperatives: Mojosongo cooperatives, Musuk cooperative, and Cepogo cooperatives using the survey method. Total respondents used in this study were 266 farmers divided into three stratum. Primary data was collected through interviews based on a questionnaire that has been prepared. Data were analyzed using multiple linear analysis simple. The analysis showed that characteristics of dairy farmers, forage land and water availability together significantly influenced the milk production. At stratum R2 value of 0.965 suggesting that milk production could be explained by factors independent as much as 96.50 %, while the remaining 3.50 % is caused by other factors outside the model. At stratum II R2 value of 0.635 suggesting that milk production could be explained by factors independent as much as 63.50 %, while the remaining 35.50 % is caused by other factors outside the model. At stratum III R2 value of 0.681 suggesting that milk production could be explained by factors independent as much as 68.10 %, while the remaining 31.90 % is caused by other factors outside the model

Trend of Dairy Population an Milk Production in Boyolali, Central Java, Indonesia

This study is one part of the business development strategy research of dairy cattle in Boyolali, Central Java. This study aimed to analyze the trend of the population of dairy cattle and milk production in Boyolali, Central Java. This study was conducted between November 2010 until January 2011 in the district of Boyolali by using the survey method. Total respondents were used in this study were 266 farmers. Primary data was collected by interviews based on a questionnaire that has been prepared. Secondary data were obtained from the Central Bureau of Statistics, Department of Animal Husbandry and Fisheries, and local governments Boyolali. The average of dairy lactation ownership is 6.40 AU. The average production of cow's milk in Boyolali is still low at 8 liters/head/day. Trend dairy cow population is Y=4,7182e0,0063x, production trend equation is Y=5.8567 e0,0052x. Both equations are used to estimate the size of the population of dairy cattle and milk production in the coming years. Trend population of dairy cows, milk production is increasing every year

Dairy Contest as Selection Method for High Production Cow in Sarana Usaha Warga Sejahtera Cooperative Sleman

Community service aimed to assist farmers in selecting high-quality dairy cows. The activity was done in the location of the Sarana Usaha Warga Sejahtera cooperative. The activity was held by training, recording, and contest for heifer and lactating cows. Materials of extension and training were delivered to farmer groups by online tutorials. The dairy cow contest was done semi virtually. Assessment for quantitative traits was done by measuring body size and qualitative traits or physical appearance by observation on cow’s pictures. Score contest consisted of 60% quantitative and 40% qualitative. The participant was the best dairy cow in the group, that was selected by the group followed of the contest guideline. Each farmer group was represented in the contest by one heifer and one first lactating cow. The results showed the winner contest, both heifers and lactating cow have large body size and good physical appearance. The average body length, wither height, a chest circumference of a heifer, and lactating cow, respectively, were 150.6 and 164.7 cm, 133.8 and 138.0 cm, 179.8, and 192.0 cm. The winner reached a good physical appearance score; the average was 75.3 on heifer and 71.7 on lactating cow. In conclusion, the dairy contest was successful, showing good quality cows characteristics in the cooperative. The cow was qualitatively shown a high appearance on dairy character and good in the frame, leg, and mammary system. The contest could provide basic data for preparing the rearing program in the dairy cooperative and motivated farmers to improve productivity. Keyword: Dairy contest, selection, Cooperativ

Faktor Risiko Kejadian Mastitis pada Kambing Peranakan Etawah (PE) di Kelompok Ternak Tirto, Kokap, Kulonprogo, Yogyakarta: The Risk Factor for Mastitis in Peranakan Etawah (PE) Goat at Tirto Farmer Group, Kokap, Kulonprogo, Yogyakarta

Kelompok Ternak Tirto merupakan salah satu peternakan kambing Peranakan Etawah (PE) yang cukup besar dan berada di wilayah Kulonprogo, Yogyakarta. Kambing Peranakan Etawah dikembangbiakkan untuk produksi susu. Intra Mammary Infection (IMI) atau mastitis, khususnya mastitis subklinis, merupakan salah satu penyakit yang menurunkan produksi susu kambing. Tujuan penelitian ini adalah untuk mengetahui faktor risiko yang berkontribusi terhadap terjadinya mastitis subklinis pada kambing PE di Kelompok Ternak Tirto tersebut. Data faktor risiko dikumpulkan menggunakan kuesioner. Metode kuesioner berupa wawancara langsung kepada 19 peternak anggota kelompok ternak ini dan observasi pada saat dilakukan kegiatan pemerahan. Faktor risiko pada kambing PE ditentukan dengan menggunakan odds ratio (OR). Faktor risiko yang berhubungan positif dengan mastitis subklinis pada kambing PE kelompok tani Tirto di Kokap, Kulonprogo, Yogyakarta adalah kebersihan kandang (OR = 1,2) dan pembersihan ambing (OR = 8,6), sedangkan faktor risiko lainnya adalah asosiasi negatif