Effect of pectin on the characteristics of edible film from pink ear emperor (lethrinus lentjan) gelatin

Edible film is a thin layer used to coat food. One of the biopolymers that can be used as a base for the edible film is fish gelatin. Gelatin can be obtained from the skin of Lethrinus lentjan. However, edible film is usually brittle if only gelatin is used, so other materials, such as pectin, are needed to form an elastic film. This study aims to determine the effect of pectin addition on the characteristics of the edible film. The method used in this research was the experimental method with five treatments of pectin concentration (0%, 0.2%, 0.25%, 0.3%, 0.35%). Physical and chemical characteristics (thickness, tensile strength, elongation, water vapor transmission, and moisture content) of edible films were analyzed. The results showed that different concentrations of pectin significantly affected the characteristics of edible film. The best characteristics of edible film (0.12 mm thickness, 15.40 MPa tensile strength, 26.50% elongation, 6.99 gram/m2 24 h water vapor transmission rate, and 8.745% moisture content) were obtained as 0.2% pectin added in making the gelatin-based edible film

Dietary tuna hydrolysate modulates growth performance, immune response, intestinal morphology and resistance to Streptococcus iniae in juvenile barramundi, Lates calcarifer

This study investigated the effects of tuna hydrolysate (TH) inclusion in fishmeal (FM) based diets on the growth performance, innate immune response, intestinal health and resistance to Streptococcus iniae infection in juvenile barramundi, Lates calcarifer. Five isonitrogenous and isoenergetic experimental diets were prepared with TH, replacing FM at levels of 0% (control) 5%, 10%, 15% and 20%, and fed fish to apparent satiation three times daily for 8 weeks. The results showed that fish fed diets containing 5% and 10% TH had significantly higher final body weight and specific growth rate than the control. A significant reduction in blood glucose was found in fish fed 10%, 15% and 20% TH compared to those in the control whereas none of the other measured blood and serum indices were influenced by TH inclusion. Histological observation revealed a significant enhancement in goblet cell numbers in distal intestine of fish fed 5 to 10% TH in the diet. Moreover, fish fed 10% TH exhibited the highest resistance against Streptococcus iniae infection during a bacterial challenge trial. These findings therefore demonstrate that the replacement of 5 to 10% FM with TH improves growth, immune response, intestinal health and disease resistance in juvenile barramundi

Klasifikasi Jenis Buah Apel Menggunakan Metode Orde 1 dengan Algoritma Multi Support-Vector Machines

Apel merupakan jenis buah-buahan dimana memiliki beragam jenis warna pada kulitnya tersendiri, ada yang berwarna merah, hijau maupun warna kuning. Buah apel juga memiliki banyak jenis dan mudah ditemukan dalam negeri maupun di luar negeri. Setiap jenis buah apel memiliki ciri yang berbeda sehingga akan diperoleh pendapat yang berbeda oleh setiap individu yang menyebabkan tingkat akurasi yang berbeda. Teknologi klasifikasi saat ini dapat digunakan untuk membantu proses pemilahan tersebut. Penelitian ini menggunakan metode orde 1 dengan algoritma multi SVM yang bertujuan untuk pengenalan pola buah apel menggunakan analisis tekstur pada citra grayscale dan ekstraksi fitur warna pada citra warna. Hasil ekstrasi fitur akan menjadi input bagi multi SVM untuk mengklasifikasikan jenis apel. Data yang digunakan pada penelitian ini adalah dari 50 citra, yang terdiri dari 35 citra data latih dan 15 data uji. Hasil akurasi metode ini mencapai tingkat akurasi hanya sebesar 86%

Identification of Protease-Producing Halophilic Bacteria Isolated From Salt-Pond Soil

HighlightsThe halophilic bacteria were isolated from salt-pond soil.Isolate C2 was identified as the best protease producer.Isolate C2 was identified as B. amyloliquefaciens strain UBCAbstractProtease is an important enzyme for various industries, such as pharmaceuticals, leather production, meat processing, protein hydrolyzate, food products, and waste processing industries. This study aimed to isolate and identify protease-producing bacteria isolated from salt-pond soil at the Tuban Regency, East Java, Indonesia. Bacterial communities in the soil samples were firstly isolated from the soil samples by culture-dependent technique on Luria Bertani agar enriched with 5% NaCl. Thereafter, bacterial colonies that grew on the media were purified and screened for their protease production using a skim-milk agar. The bacterial colony which produced protease was further identified using phenotypic (gram staining) and genotypic assays (the 16S rDNA sequence). The result showed that one isolate out of six (isolate C2) obtained from the soil sample was observed to produce a protease enzyme. Based on its 16S rDNA sequence, the isolate was identified as Bacillus amyloliquefaciens strain UBC. These results suggest that B. Amyloliquefaciens strain UBC is a salt-tolerant bacterium (halophilic bacteria) which has the potential to be further developed for protease-producing biological agents

Malagasy Dye Plant Species: A Promising Source of Novel Natural Colorants with Potential Applications – A Review

International audienceDue to the potentially harmful effects of some synthetic dyes, there is an increasing demand for natural colorants. Recent literature has emphasized the necessity of investigating new sources of dyes. This review discusses the biological sources of dyes derived from the rich plant diversity of Madagascar. As one of the first contributions on the use of these dyestuffs for dyeing textiles, it provides an overview of 128 dye plant species with other potential applications for coloring materials in industry. A detailed description of the botanical and chemical properties of these dyestuffs is given. We believe that the Madagascar plant diversity may be a promising source of novel colorants not yet investigated. We considered it worthwhile to carry out a thorough scientific study of a set of Malagasy plants carefully selected for their coloring properties together with their potential use and valorization in specialized industries where use of natural colorants would be a particular interest