Oxidative Stress Investigation in Anemia

Introduction: Increased membrane rigidity, decreased deformability and hemolysis in erythrocytes are the result of oxidative damage. Our study included patients diagnosed with anemia and a control group of healthy individuals and in these groups, it was aimed to measure the level of Nitric Oxide (NO), which is an indicator of oxidative damage, and to determine the superoxide dismutase enzyme (SOD) activity, which are the most important components of the antioxidant system. Material and Method: Ethics Committee approval was obtained for the study and patients who were diagnosed with iron deficiency anemia, B12 deficiency anemia, Talassemia minor at the hematology polyclinic of the Manisa Celal Bayar University Medical Faculty Hafsa Sultan Hospital were included in the study. Nitrite / nitrate levels were measured spectrophotometrically, SOD enzyme activity was measured by ELISA method. Results: The difference between the study groups in terms of SOD values was not statistically significant. When nitric oxide results are compared; Thalassemia minor group values were significantly higher than control group (p <0.05). Conclusions: Increased levels of NO, especially in the thalassemia patient group, it is thought to be the cause of hemolytic anemia as a result of oxidative stress and lipid peroxidation in this patient group. Supporting Institutions: Manisa Celal Bayar University Scientific Researches Unit (2015-092).Keywords: Anemia, Antioxidant System, Nitric Oxide, Superoxide Dismutase.DOI: 10.7176/JHMN/75-0

Sensitively recorded breathing signals of rats and their nonlinear dynamics

Nonlinear dynamical properties of sensitively recorded breathing signals (SRBS), which include cardiac induced air flow pulsations so-called pneumocardiogram (PNCG) signals, are investigated, in this methodological study. For this purpose, we assessed the SRBS of laboratory rat. The nonlinear behaviors of SRBS were investigated by the reconstructing phase space, using the autocorrelation function and the false nearest neighbor method. The chaotic SRBS attractors were discussed from the point of view of the cardiopulmonary system. This method can be used to assess the heart performance and respiratory mechanics, and might be useful to design for the physiological studies of cardiorespiratory system in small laboratory animals. (C) 2007 Elsevier B.V. All rights reserved

Melatonin and L-carnitin improves endothelial disfunction and oxidative stress in Type 2 diabetic rats

Vascular dysfunction is thought to play a major role in the development of diabetic cardiovascular disease. The roles of endothelial dysfunction, oxidative stress, and dyslipidemia will be considered. Melatonin as well as L-carnitine were shown to possess strong antioxidant properties. Diabetes induced with high fat diet (for 8 weeks) and multipl low doses intraperitoneal injection of STZ (twice, 30 mg/kg/d i.p). The diabetic animals were randomly assigned to one of the experimental groups as follows: Control group (C), high fat diet (HFD), STZ-induced diabetic group (HFD+STZ) , HFD+STZ diabetic group received melatonin (10 mg/kg/d i.p), HFD+STZ diabetic group received L-carnitine (0.6 g/kg/d i.p), and HFD+STZ diabetic group received glibenclamide (5 mg/kg/d, oral). The serum fasting blood glucose, insulin, total cholesterol, HDL- cholesterol, LDL-cholesterol, triglyceride and malondialdehyde (MDA) levels were tested. Acetylcholine induced endothelium-dependent relaxation and sodium nitroprusside induced endothelium-independent relaxation were measured in aortas for estimating endothelial function. Also, glutathione peroxidase (GPx), superoxide dismutase (SOD) and nitric oxide (NO) levels activities were determined in rat liver. According to our results melatonin and L-carnitine treatment decreased fasting blood glucose, total cholesterol, and LDL levels. MDA levels significantly decreased with the melatonin treatment whereas SOD levels were not significantly changed between the groups. The results suggest that especially melatonin restores the vascular responses and endothelial dysfunction in diabetes

Serum IGF-1 levels correlate negatively to liver damage in diabetic rats

Diabetes and insulin resistance frequently cause liver damage. Diabetes also causes reduction in liver and blood IGF-1 levels. We investigated the relation between liver damage and IGF-1 levels in diabetic rats. Fourteen Wistar albino rats were divided into control and diabetic groups. Diabetes was induced by streptozotocin. Rats were sacrificed for biochemical and histologic examinations 2 weeks after streptozotocin injection. Serum and liver IGF-1 levels were decreased, liver malondialdehyde (MDA) levels were increased, glutathione peroxidase (GPx) enzymes activities were decreased and serum alanine aminotransferase (ALT) levels were increased in diabetic group. Microscopic examination of liver revealed that normal tissue organization was disrupted in streptozotocin-induced diabetic rats. There was a strongly positive correlation between blood glucose levels and liver injury, and blood and liver IGF-1 levels. There was a strongly negative correlation between blood IGF-1 levels and hepatic injury. Our results suggest that reduction of blood IGF-1 levels correlates with hepatic injury and circulating IGF-1 levels may have predictive value for determining hepatic damage that results from diabetes. In addition, circulating IGF-1 levels are correlated with glutathione levels and the oxidative stress status of diabetic rat liver