Evulation of Antimutagenic Activity of Ni(II) Complexes with Unsymmetric Schiff Bases

In this work, Ni(II) complexes with unsymmetric Schiff bases (NiL1, NiL2, NiL3, NiL4) were prepared by a two-stage method reported by one of us recently for investigate antimutagenic properties. Sodium azide-induced antimutagenic effect in lymphocytes was determined by sister chromatid exchange (SCE) and micronucleus (MN) methods. It has been determined that the synthesized compounds have antimutagenic properties and reduce the mutagenicity caused by sodium azide (NaN3) which is used as a positive control

Halkalı Alkil Tiyofen-Schiff Bazlarının Protonlanma ve Bunların Ni(II) İyonu ile Kompleks Oluşum Sabitlerinin Belirlenmesi

Özet: Halkalı alkil tiyofen-Schiff bazların protonlanma ve bunların Ni(II) iyonu ile olan komplekslerinin kararlılık sabitleri %50 etil alkol-su (v/v) ortamında, potansiyometrik titrasyon yöntemi ile tayin edildi. Titrasyonlar 25°C'de, azot atmosferinde ve 0,15 M KCl'lü ortamda yapıldı. Potansiyometrik titrasyon verilerinden yararlanarak PKAS programı ile Schiff bazlarının stokiyometrik protonlanma sabitleri hesaplandı. Schiff bazlarına ait protonlanma sabitlerinin, kompleksleşme ve antimikrobiyel etkilerine bağlı olup olmadığı araştırıldı. Anahtar kelimeler: Schiff bazı, Halkalı alkil, Kompleks oluşum sabitleri, Protonlanma sabitleri, Tiyofen Abstract: The protonation constants of the cycloalkyl thiophen-Schiff bases and their stability constants of the Ni(II) complexes were potentiometrically determined in a ethanol–water 50% (w/w) solution at 25°C, under N2 atmosphere and 0.15 M KCl ionic strength. The stoichiometric protonation constants have been measured by the potentiometric titration and calculations were performed by the PKAS computer software recently developed. Variations of the protonation constant of Schiff bases have been discussed in view of structural effects exerted on Ni(II)-complex and antimicrobiyal. Key words: Schiff bases, Cycloring, Complex stability constant, Protonation constants, Thiophe

Enzyme Immobilization and Optimization on Polymeric Nanospheres

Enzimler, pek çok endüstride kullanılan, maliyetleri oldukça yüksek olan önemli materyallerdir. Katalitik tepkimelerde enzimler için şartlar uygun olsa bile yüksek sıcaklık, yüksek veya düşük pH ve organik/inorganik çözücülerin varlığında enzimler aktifliklerini kolaylıkla kaybedebilirler. İmmobilizasyon işlemi ile birlikte, enzimlerin ekonomik açıdan kullanılabilirliğini sağlamak mümkündür. Bu amaçla, (aminometil) polistiren (APS) kaynaklı nanokürelere glukoz oksidaz (GOx) enzimi immobilize edildi ve optimizasyon parametreleri (pH, sıcaklık, tekrar kullanım sayısı, depolama kararlılığı, substrat derişimi) belirlendi. Serbest ve immobilize enzim için Michaelis-Menten eşitliği ile Km ve Vmak değerleri hesaplandı. Özellikle Pt(IV) iyonu içeren immobilize enzimin substratına karşı yüksek ilgi gösterdiği tespit edildi. 12 ay sonunda immobilize enzimlerin yaklaşık % 85 aktifliklerini korudukları görüldü.Enzymes are important materials that are used in many industries and they have a very high cost. Although the conditions for the enzymes in catalytic reactions are appropriate, enzymes can easily lose their activity in the presence of high temperature, high or low pH and organic / inorganic solvents. With the immobilization process, it is possible to ensure that the enzymes are economically usable. For this purpose, the glucose oxidase (GOx) enzyme was immobilized to aminomethyl polystyrene (APS) nanospheres and the optimization parameters (pH, temperature, reusability number, storage stability, substrate concentration) were determined. Michaelis-Menten equation for free and immobilized enzyme and Km and Vmax values were calculated. In particular, it has been found that the substrate of the immobilized enzyme containing Pt(IV) ion shows a high affinity for the substrate. After 12 months, immobilized enzymes were found to maintain about 85% of their activity

Polystyrene attached Pt(IV)–azomethine, synthesis and immobilization of glucose oxidase enzyme

Abstract: Modified polystyrene with Pt(IV)–azomethine (APS–Sch–Pt) was synthesized by means of condensation and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on it. The characteristics of the immobilized glucose oxidase (APS–Sch–Pt–GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7. The insertion of stable Pt(IV)–azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS–Sch–Pt–GOx), increases the enzymes ’ activity and improves their affinity towards the substrate even at pH = 4. The influence of temperature, reusability and storage capacity on the free and immobilized glucose oxidase enzyme was investigated. The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 °C

Ensuring traceability of organophosphate pesticides (OPs) through enzyme immobilized spheres

Currently, pesticide residues increasingly lead to health problems. Acetylcholinesterase enzyme (AChE) plays a role in detoxification processes due to their ability to scavenge organophosphates. Thus, this enzyme has been selected for the detection of pesticides. Herein, this study describes the preparation and application of appropriate new immobilized spheres that could be used for the detection of organophosphorus pesticide residues. That is a simple colorimetric enzymatic assay for the practical discovery of N-(Mercaptomethyl) phthalimide S-(O, O-dimethylphosphorodithioate) from organophosphorus pesticides (OPs) widely used in the treatment of sugar beet, apple, nut, corn, and tobacco. The process is based on the immobilization of acetylcholinesterase onto tryptophan FMPS-Trp, (FMPS-Trp)Pd(II), and (FMPS-Trp)Pt(II) functionalized spheres. Spectroscopic and microscopic techniques were used in the characterization of the spheres where scanning electron microscopy (SEM) is an essential tool. The (FMPS-Trp)Pt(II)-AChE showed a good affinity to acetylthiocholine chloride (ATCl) and was found to have the ability to catalyze the hydrolysis of ATCl with an apparent Michaelis-Menten constant value of 288 mM. The developed colorimetric process showed good qualitative analytical performance for Phosmet (OPs) detection as low as 10(-7) M. [GRAPHICS] .Gazi University Research FundGazi University [05/2014-02]This work was supported by the Gazi University Research Fund (05/2014-02).WOS:0006122923000012-s2.0-8509995428

Evulation of Antimutagenic Activity of Ni(II) Complexes with Unsymmetric Schiff Bases

In this work, Ni(II) complexes with unsymmetric Schiff bases (NiL1, NiL2, NiL3, NiL4) were prepared by a twostage method reported by one of us recently for investigate antimutagenic properties. Sodium azide-inducedantimutagenic effect in lymphocytes was determined by sister chromatid exchange (SCE) and micronucleus (MN)methods. It has been determined that the synthesized compounds have antimutagenic properties and reduce themutagenicity caused by sodium azide (NaN3) which is used as a positive control.Bu çalışmada, asimetrik Ni(II) kompleksleri (NiL1, NiL2, NiL3, NiL4) potansiyel antimutajen özelliklerini incelemek için son zamanlarda grubumuzdan biri tarafından rapor edilen yeni iki aşamalı bir yöntem ile hazırlandı. Lenfositlerdeki sodyum azid kaynaklı antimutagenik etki, kardeş kromatid değişimi (SCE) ve mikronükleus (MN) yöntemleriyle belirlendi. Sentezlenen bileşiklerin antimutagenik özelliklere sahip olduğu ve pozitif kontrol olarak kullanılan sodyum azid (NaN3) 'ün neden olduğu mutajeniteyi azalttıkları belirlenmiştir

Anti-genotoxic Effects of Schiff bases and their Mn(III) Complexes Containing L-Aspartic acid and L-Phenylalanine

WOS: 000434277000007The purpose of the research was to evaluate the genotoxic and anti-genotoxic properties of Schiff bases and their Mn (III) complexes containing L-aspartic acid, and L-phenylalanine. The anti-genotoxic properties of four compounds in human lymphocytes cells were investigated by sister chromatid exchanges (SCEs) test system against aflatoxin Bi (AFBi). The results showed that compounds have strong anti-genotoxic properties.Gazi University Scientific Research FundGazi University [05/2010-03]The authors thank to the Gazi University Scientific Research Fund (Project number: 05/2010-03) for the financial support provided for this study

Anti-genotoxic Effects of Schiff bases and their Mn(III) Complexes Containing L-Aspartic acid and L-Phenylalanine

WOS: 000434277000007The purpose of the research was to evaluate the genotoxic and anti-genotoxic properties of Schiff bases and their Mn (III) complexes containing L-aspartic acid, and L-phenylalanine. The anti-genotoxic properties of four compounds in human lymphocytes cells were investigated by sister chromatid exchanges (SCEs) test system against aflatoxin Bi (AFBi). The results showed that compounds have strong anti-genotoxic properties.Gazi University Scientific Research FundGazi University [05/2010-03]The authors thank to the Gazi University Scientific Research Fund (Project number: 05/2010-03) for the financial support provided for this study

Nanospheres caped Pt(II) and Pt (IV): synthesis and evaluation as antimicrobial and Antifungal Agent

Antimicrobial and antifungal polymers are gaining the attention of pharmaceutical makers and industrial design. Nanospheres-Polymers attached Platinum(II) / (IV) complexes have been synthesized to investigate antimicrobial activities. Firstly, nanospheres involving Schiff bases were synthesized from (aminomethyl) polystyrene and four substitute salicylaldehyde (2-hydroxy benzaldehyde, 5-fluoro-2-hydroxy benzaldehyde, 5-kloro-2-hydroxy benzaldehyde, 5-bromo-2-hydroxy benzaldehyde). Secondly, polymers attached Platinum(II) / (IV) complexes have been prepared by means of template method. The IR spectra show that the ligands act in a monovalent bidentate fashion all nanospheres involving Schiff bases. Square-planar and octahedral structures are proposed for Pt(II) and Pt(IV), respectively. All these substances have been examined for antibacterial activity against pathogenic strains, and antifungal activity. In particular, Pt(IV) complexes were more potent bactericides than all of the synthesized substances