Upaya Optimasi Pembuatan Plasma Kaya trombosit Sebagai Pengobatan Sel Punca

Stem cells have a remarkable potential to act as a self renewal in the related system. The proliferation of stem cells can be stimulated by Platelet Rich Plasma (PRP) growth factor. PRP has a potential application for aesthetics, traumatology and maxillofacial surgery. The optimation method could be carried out by a double centrifugation, which is reliable and simple in producing an optimal PRP. The samples were obtained from healthy volunteers; 10 mL of blood was needed to produce 1 mL PRP. CBC test was then performed, in order to know the platelet count before and after double centrifugation. This method used four variations of speed and time. Four variations of speed and time were used to find the optimation result, which yield the highest platelet count and the highest PDGF level released after platelet activation. The measurement of Platelet Derived Growth Factor (PDGF-BB) level was done using ELISA method. Twenty first samples, resulted in a variation of platelet counts, the mean was 0.89 times. About 60% of platelet counts showed a decrease with the lowest value 0.01 times and 40% of the platelet counts showed an increase with the highest value 3.94 times. The study was repeated using “I” tubes, the highest increase of platelet count and PDGF-BB level was obtained by centrifugation at 900 g, 5 minutes duration, then followed by 1500 g, for 15 minutes. In this study protocol, the production of optimal PRP was not yet found, however it provided some important information. In this case, the influence of separation process and skill was more important than the centrifugation speed. The researchers suggested that the measurement of PDGF-BB level should be done immediately after PRP harvest

Arthroscopic pull-through suture for tibial eminence avulsion fracture: a case series

Fractures of the tibial eminence is commonly reported in adolescents and adults, due to traffic accident, sports injury, or any other mechanism. Case 1: a 12-year-old male complained knee pain after a traffic accident 2 months prior to visit. Case 2: a 31-year-old female complained knee pain which worsened with long-distance walking and sitting, after falling on her knees one month prior to visit. Case 3: a 27-year-old female complained a worsening knee pain after traffic accident 12 years ago. Although it has been extensively studied, controversies regarding the best fixation method still exist. Open technique was once popular, but some morbidity has been associated with this method. Therefore, a new approach using arthroscopic pull-through suture technique, albeit technically challenging, is currently being advocated as a treatment option for such fractures. The result in our series confirm that all of 3 patients have a good result based on international knee documentation committee (IKDC) scoring for evaluation the treatment, hence support the use of this novel technique for the patients with tibial eminence avulsion fracture

EAACI Molecular Allergology User's Guide 2.0

Since the discovery of immunoglobulin E (IgE) as a mediator of allergic diseases in 1967, our knowledge about the immunological mechanisms of IgE-mediated allergies has remarkably increased. In addition to understanding the immune response and clinical symptoms, allergy diagnosis and management depend strongly on the precise identification of the elicitors of the IgE-mediated allergic reaction. In the past four decades, innovations in bioscience and technology have facilitated the identification and production of well-defined, highly pure molecules for component-resolved diagnosis (CRD), allowing a personalized diagnosis and management of the allergic disease for individual patients. The first edition of the "EAACI Molecular Allergology User's Guide" (MAUG) in 2016 rapidly became a key reference for clinicians, scientists, and interested readers with a background in allergology, immunology, biology, and medicine. Nevertheless, the field of molecular allergology is moving fast, and after 6 years, a new EAACI Taskforce was established to provide an updated document. The Molecular Allergology User's Guide 2.0 summarizes state-of-the-art information on allergen molecules, their clinical relevance, and their application in diagnostic algorithms for clinical practice. It is designed for both, clinicians and scientists, guiding health care professionals through the overwhelming list of different allergen molecules available for testing. Further, it provides diagnostic algorithms on the clinical relevance of allergenic molecules and gives an overview of their biology, the basic mechanisms of test formats, and the application of tests to measure allergen exposure.Peer reviewe