The Chalcidoidea bush of life: evolutionary history of a massive radiation of minute wasps.

Chalcidoidea are mostly parasitoid wasps that include as many as 500 000 estimated species. Capturing phylogenetic signal from such a massive radiation can be daunting. Chalcidoidea is an excellent example of a hyperdiverse group that has remained recalcitrant to phylogenetic resolution. We combined 1007 exons obtained with Anchored Hybrid Enrichment with 1048 ultra-conserved elements (UCEs) for 433 taxa including all extant families, >95% of all subfamilies, and 356 genera chosen to represent the vast diversity of the superfamily. Going back and forth between the molecular results and our collective knowledge of morphology and biology, we detected bias in the analyses that was driven by the saturation of nucleotide data. Our final results are based on a concatenated analysis of the least saturated exons and UCE datasets (2054 loci, 284 106 sites). Our analyses support an expected sister relationship with Mymarommatoidea. Seven previously recognized families were not monophyletic, so support for a new classification is discussed. Natural history in some cases would appear to be more informative than morphology, as illustrated by the elucidation of a clade of plant gall associates and a clade of taxa with planidial first-instar larvae. The phylogeny suggests a transition from smaller soft-bodied wasps to larger and more heavily sclerotized wasps, with egg parasitism as potentially ancestral for the entire superfamily. Deep divergences in Chalcidoidea coincide with an increase in insect families in the fossil record, and an early shift to phytophagy corresponds with the beginning of the "Angiosperm Terrestrial Revolution". Our dating analyses suggest a middle Jurassic origin of 174 Ma (167.3-180.5 Ma) and a crown age of 162.2 Ma (153.9-169.8 Ma) for Chalcidoidea. During the Cretaceous, Chalcidoidea may have undergone a rapid radiation in southern Gondwana with subsequent dispersals to the Northern Hemisphere. This scenario is discussed with regard to knowledge about the host taxa of chalcid wasps, their fossil record and Earth's palaeogeographic history

Sex specific associations in genome wide association analysis of renal cell carcinoma.

Renal cell carcinoma (RCC) has an undisputed genetic component and a stable 2:1 male to female sex ratio in its incidence across populations, suggesting possible sexual dimorphism in its genetic susceptibility. We conducted the first sex-specific genome-wide association analysis of RCC for men (3227 cases, 4916 controls) and women (1992 cases, 3095 controls) of European ancestry from two RCC genome-wide scans and replicated the top findings using an additional series of men (2261 cases, 5852 controls) and women (1399 cases, 1575 controls) from two independent cohorts of European origin. Our study confirmed sex-specific associations for two known RCC risk loci at 14q24.2 (DPF3) and 2p21(EPAS1). We also identified two additional suggestive male-specific loci at 6q24.3 (SAMD5, male odds ratio (ORmale) = 0.83 [95% CI = 0.78-0.89], Pmale = 1.71 × 10-8 compared with female odds ratio (ORfemale) = 0.98 [95% CI = 0.90-1.07], Pfemale = 0.68) and 12q23.3 (intergenic, ORmale = 0.75 [95% CI = 0.68-0.83], Pmale = 1.59 × 10-8 compared with ORfemale = 0.93 [95% CI = 0.82-1.06], Pfemale = 0.21) that attained genome-wide significance in the joint meta-analysis. Herein, we provide evidence of sex-specific associations in RCC genetic susceptibility and advocate the necessity of larger genetic and genomic studies to unravel the endogenous causes of sex bias in sexually dimorphic traits and diseases like RCC

Genetic Variants Related to Longer Telomere Length are Associated with Increased Risk of Renal Cell Carcinoma.

BACKGROUND: Relative telomere length in peripheral blood leukocytes has been evaluated as a potential biomarker for renal cell carcinoma (RCC) risk in several studies, with conflicting findings. OBJECTIVE: We performed an analysis of genetic variants associated with leukocyte telomere length to assess the relationship between telomere length and RCC risk using Mendelian randomization, an approach unaffected by biases from temporal variability and reverse causation that might have affected earlier investigations. DESIGN, SETTING, AND PARTICIPANTS: Genotypes from nine telomere length-associated variants for 10 784 cases and 20 406 cancer-free controls from six genome-wide association studies (GWAS) of RCC were aggregated into a weighted genetic risk score (GRS) predictive of leukocyte telomere length. OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS: Odds ratios (ORs) relating the GRS and RCC risk were computed in individual GWAS datasets and combined by meta-analysis. RESULTS AND LIMITATIONS: Longer genetically inferred telomere length was associated with an increased risk of RCC (OR=2.07 per predicted kilobase increase, 95% confidence interval [CI]:=1.70-2.53, p0.5) with GWAS-identified RCC risk variants (rs10936599 and rs9420907) from the telomere length GRS; despite this exclusion, a statistically significant association between the GRS and RCC risk persisted (OR=1.73, 95% CI=1.36-2.21, p<0.0001). Exploratory analyses for individual histologic subtypes suggested comparable associations with the telomere length GRS for clear cell (N=5573, OR=1.93, 95% CI=1.50-2.49, p<0.0001), papillary (N=573, OR=1.96, 95% CI=1.01-3.81, p=0.046), and chromophobe RCC (N=203, OR=2.37, 95% CI=0.78-7.17, p=0.13). CONCLUSIONS: Our investigation adds to the growing body of evidence indicating some aspect of longer telomere length is important for RCC risk. PATIENT SUMMARY: Telomeres are segments of DNA at chromosome ends that maintain chromosomal stability. Our study investigated the relationship between genetic variants associated with telomere length and renal cell carcinoma risk. We found evidence suggesting individuals with inherited predisposition to longer telomere length are at increased risk of developing renal cell carcinoma

Exploring the Decisional Process behind Alcohol Use: Converging Evidence Across Multiple Theories

Understanding the etiological and maintaining processes of problematic drinking continues to be a challenge. There has been a growing amount of research focusing on the decisional processes that act to maintain addictive behaviors. Elucidating this underlying process is key to understanding the range of drinking behavior observed among individuals. Rather than relying on one theory, examining overlap between multiple theories of alcohol use may lead to a better understanding of such a process. Using a construct validation approach, this study utilized motivational (Ambivalence Model of Craving), cognitive (Alcohol Outcome Expectancy Theory), and behavioral theories (Behavioral Economics) of alcohol use to examine the extent to which they tap into a common underlying decisional process of alcohol use behaviors. Two methods were used including establishing motivational profiles using latent profile analysis and an experimental manipulation of situational context to examine the effect of setting on constructs of interest. Results from the two studies provided partial support for the overlap between these theories as it pertains to a common underlying process

Cognitive Ability and Ambivalence toward Alcohol: An Examination of Working Memory Capacity’s Influence on Drinking Behavior

Research stemming from dual-processing theories suggest that working memory capacity may have an important role in the ability to inhibit automatic tendencies when there is the motivation to do so (Barrett, Tugade, & Engle, 2004). Ambivalence, the simultaneous desire to engage in (approach motivation) and inhibit (avoidance motivation), often occurs with problematic behaviors like alcohol abuse. The current study sought to determine whether individual differences in working memory capacity moderate the relationship between approach, avoidance and subsequent drinking behavior in a clinical sample. A total of 66 individuals with alcohol use disorder (AUD) participated in a baseline assessment of working memory capacity followed by a daily assessment of approach, avoidance, drinking behavior and situational factors (stress and self-efficacy) over a two-week monitoring period. We also explored an alternative cognitive construct (response inhibition), to determine whether it interacts with motivational states in a similar way to predict drinking behavior. Results of multilevel modeling indicated a significant interaction between approach, avoidance and working memory capacity in the prediction of drinking day, but not drinking quantity. Specifically, those with lower working memory capacity were at increased odds of a drinking episode when experiencing ambivalence, while odds of drinking did not increase for those with higher working memory capacity. Exploratory analyses demonstrated the opposite pattern of results when examining response inhibition’s interaction with approach and avoidance. The current study suggests that working memory capacity is an important cognitive ability that supports deliberative decision making when experiencing ambivalence toward alcohol, while also highlighting important theoretical and methodological considerations when examining cognitive and motivational processes

Cognitive Ability and Ambivalence toward Alcohol: An Examination of Working Memory Capacity’s Influence on Drinking Behavior

Research stemming from dual-processing theories suggest that working memory capacity may have an important role in the ability to inhibit automatic tendencies when there is the motivation to do so (Barrett, Tugade, & Engle, 2004). Ambivalence, the simultaneous desire to engage in (approach motivation) and inhibit (avoidance motivation), often occurs with problematic behaviors like alcohol abuse. The current study sought to determine whether individual differences in working memory capacity moderate the relationship between approach, avoidance and subsequent drinking behavior in a clinical sample. A total of 66 individuals with alcohol use disorder (AUD) participated in a baseline assessment of working memory capacity followed by a daily assessment of approach, avoidance, drinking behavior and situational factors (stress and self-efficacy) over a two-week monitoring period. We also explored an alternative cognitive construct (response inhibition), to determine whether it interacts with motivational states in a similar way to predict drinking behavior. Results of multilevel modeling indicated a significant interaction between approach, avoidance and working memory capacity in the prediction of drinking day, but not drinking quantity. Specifically, those with lower working memory capacity were at increased odds of a drinking episode when experiencing ambivalence, while odds of drinking did not increase for those with higher working memory capacity. Exploratory analyses demonstrated the opposite pattern of results when examining response inhibition’s interaction with approach and avoidance. The current study suggests that working memory capacity is an important cognitive ability that supports deliberative decision making when experiencing ambivalence toward alcohol, while also highlighting important theoretical and methodological considerations when examining cognitive and motivational processes

Examination of Approach and Avoidance Inclinations on the Reinforcing Value of Alcohol

Although behavioral economics tends to focus on environmental factors (i.e., price, availability) that act to influence valuation of alcohol, recent research has begun to address how motivational and cognitive factors influence an individual\u27s demand for alcohol. Motivational states, including craving, are one possible mechanism underlying the value based decision making that demand represents. Using a multidimensional model of craving (Ambivalence Model of Craving), the current study examined the relationships between indices of alcohol demand (i.e., reinforcing value of alcohol) and craving (i.e., approach inclinations), and the ways in which competing desires moderate that relationship (i.e., avoidance inclinations). Individuals who reported consuming alcohol in the past month were recruited for the study using Amazon\u27s Mechanical Turk. A total of 529 participants (mean age = 33.03 years, SD = 8.85) completed a series of surveys assessing their drinking behavior and other alcohol-related measures. Multiple regression analyses indicated that while approach significantly predicted intensity (i.e., consumption at zero cost), Omax (i.e., the maximum alcohol expenditure) and breakpoint (i.e., the first price that seizes consumption), avoidance moderated the relationship between approach and Omax and breakpoint. Specifically, follow up analyses demonstrated that higher avoidance inclinations attenuated the effect of approach inclinations on these demand indices. Finally, despite conceptual overlap between approach, avoidance, and alcohol demand, regression analyses indicated that these constructs account for unique variance in alcohol outcomes. These results illustrate the importance of considering the effects of both approach and avoidance inclinations on an individual\u27s valuation of alcohol

Examination of Approach and Avoidance Inclinations on the Reinforcing Value of Alcohol

Although behavioral economics tends to focus on environmental factors (i.e., price, availability) that act to influence valuation of alcohol, recent research has begun to address how motivational and cognitive factors influence an individual\u27s demand for alcohol. Motivational states, including craving, are one possible mechanism underlying the value based decision making that demand represents. Using a multidimensional model of craving (Ambivalence Model of Craving), the current study examined the relationships between indices of alcohol demand (i.e., reinforcing value of alcohol) and craving (i.e., approach inclinations), and the ways in which competing desires moderate that relationship (i.e., avoidance inclinations). Individuals who reported consuming alcohol in the past month were recruited for the study using Amazon\u27s Mechanical Turk. A total of 529 participants (mean age = 33.03 years, SD = 8.85) completed a series of surveys assessing their drinking behavior and other alcohol-related measures. Multiple regression analyses indicated that while approach significantly predicted intensity (i.e., consumption at zero cost), Omax (i.e., the maximum alcohol expenditure) and breakpoint (i.e., the first price that seizes consumption), avoidance moderated the relationship between approach and Omax and breakpoint. Specifically, follow up analyses demonstrated that higher avoidance inclinations attenuated the effect of approach inclinations on these demand indices. Finally, despite conceptual overlap between approach, avoidance, and alcohol demand, regression analyses indicated that these constructs account for unique variance in alcohol outcomes. These results illustrate the importance of considering the effects of both approach and avoidance inclinations on an individual\u27s valuation of alcohol

Development and Psychometric Evaluation of a Brief Approach and Avoidance of Alcohol Questionnaire

Background: The Approach and Avoidance of Alcohol Questionnaire (AAAQ) was developed as a measure of craving to assess both desires to consume and desires to avoid consuming alcohol. Although the measure has been used in a variety of populations to predict future alcohol use behavior, the factor structures observed vary based on sample type (e.g., clinical vs. college samples) and may be overly long for use in repeated measures designs. The current article describes the development of a brief version of the AAAQ for use in clinical populations. Methods: Using existing data sets of individuals in treatment for alcohol use disorder, exploratory analyses (e.g., exploratory factor analysis and item response theory) were conducted using an inpatient sample (N = 298) at a substance abuse treatment facility. Confirmatory analyses (e.g., confirmatory factor analysis and multiple regression) were conducted using an inpatient detoxification sample (N = 175) and a longitudinal outpatient treatment sample (N = 53). Results: The brief AAAQ had comparable internal consistency, explained a similar amount of variance in alcohol consumption and related problems, and exhibited superior model fit as compared to the original measure. Conclusions: These findings indicate that the brief AAAQ is an effective tool to assess alcohol craving in clinical populations in treatment settings

Impact of Pretreatment Change on Mechanism of Behavior Change Research: An Applied Example Using Alcohol Abstinence Self-Efficacy

Objective: With the growing recognition that, for some, significant changes in drinking occur before the first treatment session (i.e., pretreatment change), researchers have called for the careful assessment of when change occurs and its potential impact on mechanism of behavior change (MOBC) research. Using a commonly hypothesized MOBC variable, alcohol abstinence self-efficacy, the primary aim of this study was to examine the effect of pretreatment change on the study of MOBCs. Method: Sixty-three individuals diagnosed with alcohol dependence were recruited to participate in a 12-week cognitive-behavioral treatment. Participants completed weekly assessments of self-efficacy and drinking behaviors. Results: Multilevel time-lagged regression models indicated that pretreatment change significantly moderated the effect of self-efficacy on the number of drinking days, such that among those higher on pretreatment change, higher self-efficacy ratings predicted lower rates of drinking days in the week until the next treatment session. In contrast, pretreatment change did not moderate the effect of self-efficacy on the rate of heavy drinking days. Conclusions: Results from the current study add to a small but growing body of research highlighting the importance of pretreatment change when studying MOBCs. Further, these results provide important insights into the conditions in which self-efficacy may play an important role in treatment outcomes