The accuracy of chromosomal microarray testing for identification of embryonic mosaicism in human blastocysts

A dança é uma forma ancestral de magia, invenção dos deuses que a ensinaram aos homens, diz-nos a mitologia hindu. Envolvido no mistério e movimento da dança, o dançarino pode encantar; porém, antes de tudo é preciso que encante a si mesmo. Não seria este também o caminho do professor? Fazer para si para poder fazer ou propor aos educandos, encantar-se para poder encantar; criar para poder seguir com as crianças a aventura da criação; ousar para poder encorajar? Nesta direção, a pergunta que percorre o presente artigo é assim formulada: como contribuir com o processo de encantamento dos professores, como alimentar a sensibilidade, nos percursos da formação universitária? Buscando respostas no processo de pesquisa, identifica-se na experiência com as danças circulares, tradição de diferentes povos, um profícuo caminho pelo qual aquele espaço de encantamento, de inteireza, de educação estética, igualmente, pode ser provocado

Quantitative Proteomics of Intracellular Campylobacter jejuni Reveals Metabolic Reprogramming

Campylobacter jejuni is the major cause of bacterial food-borne illness in the USA and Europe. An important virulence attribute of this bacterial pathogen is its ability to enter and survive within host cells. Here we show through a quantitative proteomic analysis that upon entry into host cells, C. jejuni undergoes a significant metabolic downshift. Furthermore, our results indicate that intracellular C. jejuni reprograms its respiration, favoring the respiration of fumarate. These results explain the poor ability of C. jejuni obtained from infected cells to grow under standard laboratory conditions and provide the bases for the development of novel anti microbial strategies that would target relevant metabolic pathways

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Identification of Campylobacter jejuni Genes Involved in Its Interaction with Epithelial Cells▿ †

Campylobacter jejuni is the leading cause of infectious gastroenteritis in industrialized nations. Its ability to enter and survive within nonphagocytic cells is thought to be very important for pathogenesis. However, little is known about the C. jejuni determinants that mediate these processes. Through an extensive transposon mutagenesis screen, we have identified several loci that are required for C. jejuni efficient entry and survival within epithelial cells. Among these loci, insertional mutations in aspA, aspB, and sodB resulted in drastic reduction in C. jejuni entry and/or survival within host cells and a severe defect in colonization in an animal model. The implications of these findings for the understanding of C. jejuni-host cell interactions are discussed

Characterization of a Campylobacter jejuni VirK Protein Homolog as a Novel Virulence Determinant▿ †

Campylobacter jejuni is a leading cause of food-borne illness in the United States. Despite significant recent advances, its mechanisms of pathogenesis are poorly understood. A unique feature of this pathogen is that, with some exceptions, it lacks homologs of known virulence factors from other pathogens. Through a genetic screen, we have identified a C. jejuni homolog of the VirK family of virulence factors, which is essential for antimicrobial peptide resistance and mouse virulence

A MyD88-Deficient Mouse Model Reveals a Role for Nramp1 in Campylobacter jejuni Infection

Campylobacter jejuni is a major worldwide cause of enteric illnesses. Adult immunocompetent mice are not susceptible to C. jejuni infection. However, we show here that mice deficient in the adaptor protein myeloid differentiation factor 88 (MyD88), which is required for signaling through most Toll-like receptors, can be stably colonized by C. jejuni but not by isogenic derivatives carrying mutations in known virulence genes. We also found that Nramp1 deficiency increases the mouse susceptibility to C. jejuni infection when administered systemically. These results indicate that MyD88-deficient mice could be a useful model to study C. jejuni colonization and reveal a potential role for Nramp1 in the control of this bacterial pathogen

SRM measurements of a subset of <i>C. jejuni</i> metabolic enzymes.

1<p>For each peptide, three SRM transitions were measured.</p>2<p>Intensity values were represented by integrated peak area obtained from extracted ion chromatograms.</p>3<p>Fold change of fragment ion intensities between the 2 h and 20 h samples; negative values denote decreased levels in the 20 h samples.</p>4<p>Spectral counts obtained for the same biological replicate.</p>5<p>Fold change of peptide spectral counts between the 2 h and 20 h samples; negative values denote decreased levels in the 20 h samples.</p>6<p>Peak not detected due to low peptide ion signals.</p>7<p>Fold change not determined. In this case, a large negative fold value would be expected.</p

Levels of <i>C. jejuni</i> proteins associated with oxidative stress.

1<p>Protein abundance is indicated as averaged spectral counts from 7 biological replicates.</p>2<p><i>C. jejuni</i> samples isolated from host cells at 2 h of infection.</p>3<p><i>C. jejuni</i> samples isolated from host cells at 20 h of infection.</p>4<p>Fold change in protein abundance; positive or negative values indicate higher or lower levels in the 20 h samples, respectively.</p>5<p>p-values were calculated using the paired Student's <i>t</i>-test.</p>6<p>Data not available (due to low protein signal).</p

Protein abundance of respiration/electron transport chain components of <i>C. jejuni</i> in four distinct environments.

<p>Protein abundance (expressed as spectral counts) in <i>C. jejuni</i> samples obtained after growth in BHI, after 2 and 20 hs after infection, and after exposure to oxygen limiting conditions subsequently to their recovery 20 hs after infection (indicated as O₂-limiting). <b>A</b> Protein abundance of representative enzymes involved in aerobic respirations. <b>B</b> Protein abundance of representative enzymes involved in anaerobic respirations. <b>C</b> Protein abundance of enzymes relevant to fumarate respiration. Values represent the mean ± standard error of the mean of three determinations.</p