Measuring soil moisture with spaceborne synthetic aperture radar data

This report describes the methodology and preliminary results obtained within the NEE6881S Innovation Flexible Fund project funded by the British Geological Survey (BGS) aimed at assessing the capabilities of active radar satellite imagery in deriving soil moisture values. The first part of the report introduces the project in the context of the most recent methodologies used to assess soil moisture with a particular focus on spaceborne technologies. The second part details the datasets and workflow adopted for the two case studies chosen in this work: Chobham Common and Hollin Hill, both in the UK. Around 1.7Tb of Synthetic Aperture Radar (SAR) imagery from Senintel-1 satellite have been processed to detect changes of the hydrological conditions at the two sites for the 2015-2018 period. The backscattering coefficient retrieved from Sentinel-1 images has then been compared with ground truth data on the Volumetric Water Content (VWC) and analysed against the ZOODRM recharge model. The main findings are that: the SAR signal has been able to penetrate down to a maximum depth of 15 cm in the terrain (i), the best correlation with the VWC changes is observed with the vertical transmit – vertical receive polarization of the SAR antenna (ii) and for every unit change in the backscatter signal, VWC varies by about 25% to 33% at Chobham Common and ~20% to ~50% at Hollin Hill which translate into a sensitivity of 0.04 dB/[vol.%] to 0.03 dB/[vol.%] and 0.05 dB/[vol.%] to 0.02 dB/[vol.%], respectively. The Discussion and Conclusions detail the significance and benefits of these findings, current limitations in our methodology and how it can be improved

Connecting Neurons to a Mobile Robot: An In Vitro Bidirectional Neural Interface

One of the key properties of intelligent behaviors is the capability to learn and adapt to changing environmental conditions. These features are the result of the continuous and intense interaction of the brain with the external world, mediated by the body. For this reason “embodiment” represents an innovative and very suitable experimental paradigm when studying the neural processes underlying learning new behaviors and adapting to unpredicted situations. To this purpose, we developed a novel bidirectional neural interface. We interconnected in vitro neurons, extracted from rat embryos and plated on a microelectrode array (MEA), to external devices, thus allowing real-time closed-loop interaction. The novelty of this experimental approach entails the necessity to explore different computational schemes and experimental hypotheses. In this paper, we present an open, scalable architecture, which allows fast prototyping of different modules and where coding and decoding schemes and different experimental configurations can be tested. This hybrid system can be used for studying the computational properties and information coding in biological neuronal networks with far-reaching implications for the future development of advanced neuroprostheses

Thiazolidin-4-one formation. Mechanistic and synthetic aspects of the reaction of imines and mercaptoacetic acid under microwave and conventional heating

Microwave irradiation of a mixture of benzylidene-anilines and mercaptoacetic acid in benzene gives 1,3-thiazolidin-4- ones in very high yield (65–90%), whereas the same reaction performed through using the conventional method, at refluxtemperature, requires a much longer time and gives a much lower yield (25–69%). This difference seems to be due to someintermediates and by-products formed during the conventional reaction. On the basis of 1H NMR studies, two differentmechanisms, acting in benzene and in DMF, respectively, have been hypothesized for the thiazolidin-4-one system formation

Reaction between quinone and thiazolidine. A study on the formation mechanism of new antiproliferative quinolindiones

Reaction between quinolinquinone and thiazolidine in basic medium was investigated. 2-Arylthiazolidine-4-carboxylic acid ethyl esters undergo two different cleavages in basic medium, yielding the 1-aryl-2-azadiene and a thiolic species. In the presence of quinolinquinone, the isomeric 1-aryl-3-ethoxycarbonyl-pyridoisoquinolin-5,10-diones and 3-amino-3-ethoxycarbonyl-dihydrothienoquinolin- 4,9-diones are formed by a hetero-Diels–Alder reaction and 1,4-Michael addition reaction, respectively. A mechanism for the formation of the reaction products is presented

Genotoxicity assessment of three nutraceuticals containing natural antioxidants extracted from agri-food waste biomasses

Grapes and apples are the most cultivated fruits in the Mediterranean basin and their agricultural processing is responsible for the production of a large amount of bio-waste. The reuse of this food biomass would increase the volume of recyclable/renewable biomaterial and lower the environmental impact due to the increasing demand for these biological products. To this purpose, agri-food waste from grape and apple processing have become an important source of phytochemicals, and many pharmaceutical industries are using it as starting material to produce dietary supplements, functional foods, and food additives for human consumption. In virtue of the chemical diversity and complexity of agri-food biowaste, developers and producers of nutraceuticals are advised to assess the safety of their final nutraceutical products, in compliance with European Food Safety Authority regulation. Here, we use the Ames test to assess the mutagenicity of three nutraceuticals obtained from agri-food waste biomasses: Taurisolo® from grape pomace of Vitis vinifera L. cv 'Aglianico', AnnurComplex® from Malus pumila M. cv 'Annurca' and Limoncella Apple Extract from Malus domestica B. cv 'Limoncella'. The results showed that all three nutraceuticals were non-mutagenic

SAR data and field surveys combination to update rainfall-induced shallow landslide inventory

The Campania region has been recurrently hit by severe landslides in volcanoclastic deposits. The city of Naples, and in particular the Camaldoli and Agnano hills (Phlegraean Fields), also suffered several landslide crises in weathered volcanoclastic rocks as a consequence of intense rainfalls or wildfires. To identify slope failures phenomena occurred in the winter season 2019–2020 an innovative procedure has been proposed. The purpose of this procedure is to highlight areas where major land cover changes occurred within our area of study, which can be potentially related to mass movements. The amplitude of spaceborne SAR images has been exploited for the change detection analysis and the output derived from the segmentation procedure has been compared with field observations. The amplitude-based method has been already applied in the detection of landslides, but never on the event with limited extensions, such as for this application. The achieved outcomes allowed the mapping of 62 new landslides that have been used to update the current landslide inventory database. This type of information is expected to help decision-makers with land planning and risk assessment

Biophysical and biochemical characterization of a liposarcoma-derived recombinant MnSOD protein acting as an anticancer agent

A recombinant MnSOD (rMnSOD) synthesized by specific cDNA clones derived from a liposarcoma cell line was shown to have the same sequence as the wild-type MnSOD expressed in the human myeloid leukaemia cell line U937, except for the presence of the leader peptide at the N-terminus. These results were fully confirmed by the molecular mass of rMnSOD as evaluated by ES/MS analysis (26662.7 Da) and the nucleotide sequence of the MnSOD cDNA. The role of the leader peptide in rMnSOD was investigated using a fluorescent and/or 68Gallium-labelled synthetic peptide. The labelled peptide permeated MCF-7 cells and uptake could be inhibited in the presence of an excess of oestrogen. In vivo it was taken up by the tumour, suggesting that the molecule can be used for both therapy and diagnosis. The in vitro and in vivo pharmacology tests confirmed that rMnSOD is only oncotoxic for tumour cells expressing oestrogen receptors. Pharmacokinetic studies in animals performed with 125I- and 131I-labelled proteins confirmed that, when administered systemically, rMnSOD selectively reached the tumour, where its presence was unambiguously demonstrated by scintigraphic and PET scans. PCR analysis revealed that Bax gene expression was increased and the Bcl2 gene was down regulated in MCF7 cells treated with rMnSOD, which suggests that the protein induces a pro-apoptotic mechanism

May Polyphenols Have a Role Against Coronavirus Infection? An Overview of in vitro Evidence

The coronavirus infection is constantly diffusing worldwide and the incidence of death is dramatically increasing, representing one of the greatest disasters in human history. Nowadays, no effective therapeutic approaches have been licensed, despite the rising interest of the scientific research in this specific field, and the daily growing number of publications, while the need to find novel strategies is urgent. Evidence in the literature reported the antiviral activity of polyphenols, the largest class of bioactive compounds in nature. Interestingly, a limited number of studies investigated the efficacy of polyphenols from different raw materials, directly against coronaviruses. The present manuscript aimed to report this evidence and provide a viewpoint on the possibility to use it as a start point for the development of novel natural approaches against this viral infection, eventually designing further appropriate researches

Cardioprotective Effects of Taurisolo® in Cardiomyoblast H9c2 Cells under High-Glucose and Trimethylamine N-Oxide Treatment via de Novo Sphingolipid Synthesis

In addition to high plasma glucose, increased levels of trimethylamine N-oxide (TMAO) have been found in obese subjects, where are considered as a novel risk factor for cardiovascular diseases. The present study aimed to investigate the effect of a novel nutraceutical formulation based on grape polyphenols (registered as Taurisolo®) in counteracting TMAO- and high glucose (HG)-induced cytotoxicity in cardiomyoblast H9c2 cells. Cell damage was induced with HG (HG-H9c2) and HG+TMAO (THG-H9c2); both experimental cell models were, thus, incubated for 72 h in the presence or absence of Taurisolo®. It was observed that Taurisolo® significantly increased the cell viability and reduced lactate dehydrogenase and aspartate transaminase release in both HG- and THG-H9c2 cells. Additionally, through its antioxidant activity, Taurisolo® modulated cell proliferation via ERK activation in THG-H9c2. Furthermore, Taurisolo® was able to induce autophagic process via increasing the expression of LC3II, a protein marker involved in formation of autophagosome and ex novo synthesis of sphingomyelin, ceramides, and their metabolites both in HG- and THG-H9c2 cells. Finally, Taurisolo® reduced hypertrophy and induced differentiation of HG-H9C2 cells into cardiomyocyte-like cells. These data suggest that Taurisolo® counteracts the toxicity induced by TMAO and HG concentrations increasing autophagic process and activating de novo sphingolipid synthesis, resulting in a morphological cell remodeling. In conclusion, our results allow speculating that Taurisolo®, combined with energy restriction, may represent a useful nutraceutical approach for prevention of cardiomyopathy in obese subjects

On resin click-chemistry-mediated synthesis of novel enkephalin analogues with potent anti-nociceptive activity

Here, we report the chemical synthesis of two DPDPE analogues 7a (NOVA1) and 7b (NOVA2). This entailed the solid-phase synthesis of two enkephalin precursor chains followed by a CuI-catalyzed azide-alkyne cycloaddition, with the aim of improving in vivo analgesic efficacy versus DPDPE. NOVA2 showed good affinity and selectivity for the μ-opioid receptor (KI of 59.2 nM, EC50 of 12.9 nM, EMax of 87.3%), and long lasting anti-nociceptive effects in mice when compared to DPDPE.University of ArizonaOpen access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]