External Benefits of Irrigation in Mountain Areas: Stakeholder Perceptions and Water Policy Implications

Irrigation contributes to land and ecosystem degradation, especially in intensive farming areas. However, in marginal areas, long-established irrigation systems also supply agroecosystem services. This study aimed to identify and prioritize the external benefits provided by irrigation in extensive grazing farms in an Italian alpine region (Aosta Valley, NW Italy). Three local stakeholder groups (land irrigation consortia members, non-farmer users of the irrigation water service, and non-user citizens) engaged in focus group discussions. The transcriptions were analyzed with an integrated subjective and computer-assisted approach. The main result of the study showed that a convergence of stakeholder opinions led to prioritization of the same four benefits, i.e., hydro-geological and land maintenance, traditional agricultural landscape conservation, biodiversity conservation, and leisure recreational activities provision. Incorporating this information into decision-making processes is relevant in marginal mountain areas, especially in light of the implementation of the water pricing policy laid down in the EU Water Framework Directive. To this end, the economic value of the external benefits should be considered along with the recovery costs for water services. Such information is essential to balance the environmental costs of irrigation and to compare the resource cost of alternative water uses

Protective role of st. John’s wort and its components hyperforin and hypericin against diabetes through inhibition of inflammatory signaling: Evidence from in vitro and in vivo studies

Diabetes mellitus is a very common chronic disease with progressively increasing prevalence. Besides the well-known autoimmune and inflammatory pathogenesis of type 1 diabetes, in many people, metabolic changes and inappropriate lifestyle favor a subtle chronic inflammatory state that contributes to development of insulin resistance and progressive loss of β-cell function and mass, eventually resulting in metabolic syndrome or overt type 2 diabetes. In this paper, we review the anti-inflammatory effects of the extract of Hypericum perforatum L. (St. John’s wort, SJW) and its main active ingredients firstly in representative pathological situations on inflammatory basis and then in pancreatic β cells and in obese or diabetic animal models. The simultaneous and long-lasting inhibition of signal transducer and activator of transcription (STAT)-1, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinases (MAPKs)/c-jun N-terminal kinase (JNK) signaling pathways involved in pro-inflammatory cytokine-induced β-cell dysfunction/death and insulin resistance make SJW particularly suitable for both preventive and therapeutic use in metabolic diseases. Hindrance of inflammatory cytokine signaling is likely dependent on the hyperforin content of SJW extract, but recent data reveal that hypericin can also exert relevant protective effects, mediated by activation of the cyclic adenosine monophosphate (cAMP)/protein kinase cAMP-dependent (PKA)/adenosine monophosphate activated protein kinase (AMPK) pathway, against high-fat-diet-induced metabolic abnormalities. Actually, the mechanisms of action of the two main components of SJW appear complementary, strengthening the efficacy of the plant extract. Careful quantitative analysis of SJW components and suitable dosage, with monitoring of possible drug–drug interaction in a context of remarkable tolerability, are easily achievable pre-requisites for forthcoming clinical applications

LAMINAR FORCED CONVECTION IN HORIZONTAL CHANNEL WITH HEAT GENERATION PLATES COOLED BY WATER

This paper describes the study of the behavior of two-dimensional and parallel horizontal flat plates with uniform internal heat generation subjected to laminar forced convection of water. The governing equations are solved numerically using the finite volume technique with Power-Law interpolating scheme and the SIMPLE algorithm. After the simulations the temperature and velocity fields were obtained for various plates spacing and fluid inlet velocities, as well as the Nusselt number values

BIOMASS EXPLOITATION FOR ENERGY SUPPLY AND QUALITY COMPOST PRODUCTION. AN EXEMPLARY CASE OF CIRCULAR ECONOMY IN THE NORTH EAST OF ITALY

The goal 12 of the 2030 Agenda for Sustainable Development takes into consideration the responsible consumption and production in the perspective of circular economy. The agri-food sector is more actively involved in these initiatives, because it offers the possibility to exploit waste and by-products, by adopting suitable biotechnologies. Such processes can be carried out either under aerobic conditions, for the production of compost, or anaerobically, for the production of biogas. In this work the case of a plant managed by Desag Ecologia, located in the municipality of Sedegliano, in the North-East of Italy, is presented. The plant started up in June 2016. Its main activity consists in exploitation of the organic fraction of municipal solid waste and urban forestry green waste coming from separate waste collection. The basin of provenance of collected materials consists not only of the province of Udine, but also of other areas of the Friuli Venezia Giulia region and other northern Italian regions. The plant ensures the production of both biogas (used in a cogeneration installation for producing electricity and heat) and quality compost, which can be used in agriculture, after submission to physico-chemical analyses to verify the end-of-waste status. In this way, the reduction of waste disposal in landfill is ensured. Thermal energy is partially recovered for the production of hot water to heat the anaerobic digester, the leachate collection tank and the plant rooms. Approximately 10% of electricity is self-consumed for the needs of the anaerobic facility, the remaining amount is fed straight into the public electricity network

Collagen processing and cuticle formation is catalysed by the astacin metalloprotease DPY-31 in free-living and parasitic nematodes

The exoskeleton or cuticle performs many key roles in the development and survival of all nematodes. This structure is predominantly collagenous in nature and requires numerous enzymes to properly fold, modify, process and cross-link these essential structural proteins. The cuticle structure and its collagen components are conserved throughout the nematode phylum but differ from the collagenous matrices found in vertebrates. This structure, its formation and the enzymology of nematode cuticle collagen biogenesis have been elucidated in the free-living nematode Caenorhabditis elegans. The dpy-31 gene in C. elegans encodes a procollagen C-terminal processing enzyme of the astacin metalloprotease or bone morphogenetic protein class that, when mutated, results in a temperature-sensitive lethal phenotype associated with cuticle defects. In this study, orthologues of this essential gene have been identified in the phylogenetically diverse parasitic nematodes Haemonchus contortus and Brugia malayi. The DPY-31 protein is expressed in the gut and secretory system of C. elegans, a location also confirmed when a B. malayi transcriptional dpy-31 promoter-reporter gene fusion was expressed in C. elegans. Functional conservation between the nematode enzymes was supported by the fact that heterologous expression of the H. contortus dpy-31 orthologue in a C. elegans dpy-31 mutant resulted in the full rescue of the mutant body form. This interspecies conservation was further established when the recombinant nematode enzymes were found to have a similar range of inhibitable protease activities. In addition, the recombinant DPY-31 enzymes from both H. contortus and B. malayi were shown to efficiently process the C. elegans cuticle collagen SQT-3 at the correct C-terminal procollagen processing site

Autoantibodies in inflammatory arthritis

Rheumatoid arthritis (RA) is a systemic chronic inflammatory disease characterized by extensive synovitis resulting in erosions of articular cartilage and marginal bone with joint destruction. The lack of immunological tolerance in RA represents the first step toward the development of autoimmunity. Susceptible individuals, under the influence of environmental factors, such as tobacco smoke, and silica exposure, develop autoimmune phenomena that result in the presence of autoantibodies. HLA and non-HLA haplotypes play a major role in determining the development of specific autoantibodies differentiating anti-citrullinated antibodies (ACPA)-positive and negative RA patients. Rheumatoid factor (RF) and ACPA are the serological markers for RA, and during the preclinical immunological phase, autoantibody titers increase with a progressive spread of ACPA antigens repertoire. The presence of ACPA represents an independent risk factor for developing RA in patients with undifferentiated arthritis or arthralgia. Moreover, anti-CarP antibodies have been identified in patients with RA as well as in individuals before the onset of clinical symptoms of RA. Several autoantibodies mainly targeting post-translational modified proteins have been investigated as possible biomarkers to improve the early diagnosis, prognosis and response to therapy in RA patients. Psoriatic arthritis (PsA) is distinguished from RA by infrequent positivity for RF and ACPA, together with other distinctive clinical features. Actually, specific autoantibodies have not been described. Recently, anti-CarP antibodies have been reported in sera from PsA patients with active disease. Further investigations on autoantibodies showing high specificity and sensibility as well as relevant correlation with disease severity, progression, and response to therapy are awaited in inflammatory arthritides

COVID-19 and genetic variants of protein involved in the SARS-CoV-2 entry into the host cells

The recent global COVID-19 public health emergency is caused by SARS-CoV-2 infections and can manifest extremely variable clinical symptoms. Host human genetic variability could influence susceptibility and response to infection. It is known that ACE2 acts as a receptor for this pathogen, but the viral entry into the target cell also depends on other proteins. The aim of this study was to investigate the variability of genes coding for these proteins involved in the SARS-CoV-2 entry into the cells. We analyzed 131 COVID-19 patients by exome sequencing and examined the genetic variants of TMPRSS2, PCSK3, DPP4, and BSG genes. In total we identified seventeen variants. In PCSK3 gene, we observed a missense variant (c.893G>A) statistically more frequent compared to the EUR GnomAD reference population and a missense mutation (c.1906A>G) not found in the GnomAD database. In TMPRSS2 gene, we observed a significant difference in the frequency of c.331G>A, c.23G>T, and c.589G>A variant alleles in COVID-19 patients, compared to the corresponding allelic frequency in GnomAD. Genetic variants in these genes could influence the entry of the SARS-CoV-2. These data also support the hypothesis that host genetic variability may contribute to the variability in infection susceptibility and severity