23 research outputs found
Effect of the fungicide Prochloraz-Mn on the cell wall structure of Verticillium fungicola
The chemical structure of the cell wall of two isolates of Verticillium fungicola collected from diseased fruit bodies of the commercial mushroom Agaricus bisporus treated with the fungicide Prochloraz-Mn was analyzed. The isolates were obtained during different periods of time and grown in the absence and presence of the LD50 values of the fungicide for V. fungicola. In addition, another V. fungicola isolate collected previous to the routine utilization of Prochloraz-Mn but grown under the same conditions was also analyzed. The overall chemical composition of the cell wall from the three isolates showed detectable differences in their basic components, with a significant decrease in the protein content in fungicide-treated cells. This inhibitory effect was partially compensated by an increase in neutral and/or aminated carbohydrates and was accompanied by appreciable modifications of polysaccharide structure, as deduced after methylation analysis and gas-liquid chromatography- mass spectrometry (GLC-MS). Moreover, differences in hyphal morphology caused by the fungicide were observed by transmission electron microscopy (TEM)
Enzymic activity of the mycoparasite Verticillium fungicola on Agaricus bisporus fruit body cell walls
Estudios comparativos del efecto del fungicida Prochloraz-Mn sobre las paredes celulares del micelio vegetativo y de los cuerpos fructíferos de Agaricus bisporus
Fungicides to control mycopathogens of commercial Agaricus bisporus, a mushroom cultivated for human consumption, are a major field of study, since these chemicals are toxic to both the host and its fungal parasites. The fungicide Prochloraz-Mn, used at its LD50 for A. bisporus, partially inhibited protein biosynthesis in the vegetative mycelial cell walls of this mushroom and caused significant changes in cell-wall polysaccharide structure, as deduced by methylation analysis and gas liquid chromatography-mass spectrometry (GLC-MS). Furthermore, the aggregated mycelial walls showed distinct alterations in their overall chemical composition following the administration of Prochloraz-Mn at the LD50 and the LD50 ×1000. As expected, GLC-MS studies indicated that the
latter dose caused more appreciable differences in polysaccharide structure. The
decrease in mushroom crop yields obtained from industrial cultures treated with
Prochloraz-Mn to control V. fungicola infection depended on the dose of the fungicide employed, whereas fruit-body morphology was only slightly affected at
the highest Prochloraz-Mn concentration used.Los fungicidas para el control de micopatógenos de Agaricus bisporus, un hongo de cultivo comercial para consumo humano, representan
un importante tema de estudio debido a que son tóxicos tanto para el huésped como para sus parásitos fúngicos. El fungicida Prochloraz-Mn,
empleado a su LD50 para A. bisporus, inhibe parcialmente la biosíntesis de proteínas en las pared celular del micelio vegetativo de este hongo y provoca cambios significativos en la estructura polisacárida de la pared celular, tal
como se observa mediante el análisis de metilación y la cromatografía líquida de gases-espectrometría de masa (GLC-MS). Además, las paredes agregadas del micelio presentan diferentes alteraciones en la composición química
global después de la administración de Prochloraz-Mn a la LD50 y LD50 ×1000. Como cabría esperar, los estudios de GLC-MS, indican que la última dosis causa más diferencias apreciables en la estructura polisacárida. La disminución en la producción del hongo en los cultivos industriales tratados con Prochloraz-Mn para controlar la infección por V. fungicola, dependía de la dosis de fungicida empleada, mientras que la morfología del cuerpo fructífero
sólo resulta ligeramente afectada a la concentración de Prochloraz-Mn más elevada.Financial support for this study was provided by the Dirección General de Investigación Científica y Técnica (CICYT, grant AGL-2001-1885)) and the Junta de Comunidades de Castilla-La Mancha
(grant 188/CH-51).Peer reviewe