Droplets Transport in a Microfluidic Chip for In Vitro Compartmentalisation

In vitro compartmentalisation is an emerging technology for protein evolution and selection. In this presentation, we will report the development of a microdrop-based microfluidic platform for in vitro enzyme evolution and selection applications. A microfluidic chip has been developed and fabricated using the standard photolithography method in conjunction with electroplating and hot embossing techniques. A cross channel geometry was used to focus liquid flows for droplet generation. To realize on-chip compartmentalised bio-reactions, two droplet generators were fabricated on the same chip. Experiments have been carried out to measure droplet size, generation rate and speed using a photographic technique. Droplet size was found to be decreasing with increasing focusing oil flow rate for a given aqueous phase flow rate. When two droplet generators are used in the same chip, the droplets may be generated asynchronously due to different flow conditions. If the droplets were significantly smaller than channel size, the faster moving droplets could pass the slower moving droplets with little coalescence. If the droplets were of the channel size, the faster moving droplets would break or fuse with the slow droplets. To achieve high rate of droplet fusion, active control should be in place for synchronous generation and fusion

Commuting Simplicity and Closure Constraints for 4D Spin Foam Models

Spin Foam Models are supposed to be discretised path integrals for quantum gravity constructed from the Plebanski-Holst action. The reason for there being several models currently under consideration is that no consensus has been reached for how to implement the simplicity constraints. Indeed, none of these models strictly follows from the original path integral with commuting B fields, rather, by some non standard manipulations one always ends up with non commuting B fields and the simplicity constraints become in fact anomalous which is the source for there being several inequivalent strategies to circumvent the associated problems. In this article, we construct a new Euclidian Spin Foam Model which is constructed by standard methods from the Plebanski-Holst path integral with commuting B fields discretised on a 4D simplicial complex. The resulting model differs from the current ones in several aspects, one of them being that the closure constraint needs special care. Only when dropping the closure constraint by hand and only in the large spin limit can the vertex amplitudes of this model be related to those of the FK Model but even then the face and edge amplitude differ. Curiously, an ad hoc non-commutative deformation of the $B^{IJ}$ variables leads from our new model to the Barrett-Crane Model in the case of Barbero-Immirzi parameter goes to infinity.Comment: 41 pages, 4 figure

Simple model for quantum general relativity from loop quantum gravity

New progress in loop gravity has lead to a simple model of `general-covariant quantum field theory'. I sum up the definition of the model in self-contained form, in terms accessible to those outside the subfield. I emphasize its formulation as a generalized topological quantum field theory with an infinite number of degrees of freedom, and its relation to lattice theory. I list the indications supporting the conjecture that the model is related to general relativity and UV finite.Comment: 8 pages, 3 figure

Hidden Quantum Gravity in 3d Feynman diagrams

In this work we show that 3d Feynman amplitudes of standard QFT in flat and homogeneous space can be naturally expressed as expectation values of a specific topological spin foam model. The main interest of the paper is to set up a framework which gives a background independent perspective on usual field theories and can also be applied in higher dimensions. We also show that this Feynman graph spin foam model, which encodes the geometry of flat space-time, can be purely expressed in terms of algebraic data associated with the Poincare group. This spin foam model turns out to be the spin foam quantization of a BF theory based on the Poincare group, and as such is related to a quantization of 3d gravity in the limit where the Newton constant G_N goes to 0. We investigate the 4d case in a companion paper where the strategy proposed here leads to similar results.Comment: 35 pages, 4 figures, some comments adde

Ponzano-Regge model revisited I: Gauge fixing, observables and interacting spinning particles

We show how to properly gauge fix all the symmetries of the Ponzano-Regge model for 3D quantum gravity. This amounts to do explicit finite computations for transition amplitudes. We give the construction of the transition amplitudes in the presence of interacting quantum spinning particles. We introduce a notion of operators whose expectation value gives rise to either gauge fixing, introduction of time, or insertion of particles, according to the choice. We give the link between the spin foam quantization and the hamiltonian quantization. We finally show the link between Ponzano-Regge model and the quantization of Chern-Simons theory based on the double quantum group of SU(2)Comment: 48 pages, 15 figure

Asymptotics of Spinfoam Amplitude on Simplicial Manifold: Euclidean Theory

We study the large-j asymptotics of the Euclidean EPRL/FK spin foam amplitude on a 4d simplicial complex with arbitrary number of simplices. We show that for a critical configuration (j_f, g_{ve}, n_{ef}) in general, there exists a partition of the simplicial complex into three regions: Non-degenerate region, Type-A degenerate region and Type-B degenerate region. On both the non-degenerate and Type-A degenerate regions, the critical configuration implies a non-degenerate Euclidean geometry, while on the Type-B degenerate region, the critical configuration implies a vector geometry. Furthermore we can split the Non-degenerate and Type-A regions into sub-complexes according to the sign of Euclidean oriented 4-simplex volume. On each sub-complex, the spin foam amplitude at critical configuration gives a Regge action that contains a sign factor sgn(V_4(v)) of the oriented 4-simplices volume. Therefore the Regge action reproduced here can be viewed as a discretized Palatini action with on-shell connection. The asymptotic formula of the spin foam amplitude is given by a sum of the amplitudes evaluated at all possible critical configurations, which are the products of the amplitudes associated to different type of geometries.Comment: 27 pages, 5 figures, references adde

Asymptotics of Spinfoam Amplitude on Simplicial Manifold: Lorentzian Theory

The present paper studies the large-j asymptotics of the Lorentzian EPRL spinfoam amplitude on a 4d simplicial complex with an arbitrary number of simplices. The asymptotics of the spinfoam amplitude is determined by the critical configurations. Here we show that, given a critical configuration in general, there exists a partition of the simplicial complex into three type of regions R_{Nondeg}, R_{Deg-A}, R_{Deg-B}, where the three regions are simplicial sub-complexes with boundaries. The critical configuration implies different types of geometries in different types of regions, i.e. (1) the critical configuration restricted into R_{Nondeg}$implies a nondegenerate discrete Lorentzian geometry, (2) the critical configuration restricted into R_{Deg-A}$ is degenerate of type-A in our definition of degeneracy, but implies a nondegenerate discrete Euclidean geometry on R_{Deg-A}, (3) the critical configuration restricted into R_{Deg-B} is degenerate of type-B, and implies a vector geometry on R_{Deg-B}. With the critical configuration, we further make a subdivision of the regions R_{Nondeg} and R_{Deg-A} into sub-complexes (with boundary) according to their Lorentzian/Euclidean oriented 4-simplex volume V_4(v), such that sgn(V_4(v)) is a constant sign on each sub-complex. Then in the each sub-complex, the spinfoam amplitude at the critical configuration gives the Regge action in Lorentzian or Euclidean signature respectively on R_{Nondeg} or R_{Deg-A}. The Regge action reproduced here contains a sign factor sgn(V_4(v)) of the oriented 4-simplex volume. Therefore the Regge action reproduced here can be viewed a discretized Palatini action with on-shell connection. Finally the asymptotic formula of the spinfoam amplitude is given by a sum of the amplitudes evaluated at all possible critical configurations, which are the products of the amplitudes associated to different type of geometries.Comment: 54 pages, 2 figures, reference adde

Cosmological Deformation of Lorentzian Spin Foam Models

We study the quantum deformation of the Barrett-Crane Lorentzian spin foam model which is conjectured to be the discretization of Lorentzian Plebanski model with positive cosmological constant and includes therefore as a particular sector quantum gravity in de-Sitter space. This spin foam model is constructed using harmonic analysis on the quantum Lorentz group. The evaluation of simple spin networks are shown to be non commutative integrals over the quantum hyperboloid defined as a pile of fuzzy spheres. We show that the introduction of the cosmological constant removes all the infrared divergences: for any fixed triangulation, the integration over the area variables is finite for a large class of normalization of the amplitude of the edges and of the faces.Comment: 37 pages, 7 figures include

Physical boundary Hilbert space and volume operator in the Lorentzian new spin-foam theory

A covariant spin-foam formulation of quantum gravity has been recently developed, characterized by a kinematics which appears to match well the one of canonical loop quantum gravity. In this paper we reconsider the implementation of the constraints that defines the model. We define in a simple way the boundary Hilbert space of the theory, introducing a slight modification of the embedding of the SU(2) representations into the SL(2,C) ones. We then show directly that all constraints vanish on this space in a weak sense. The vanishing is exact (and not just in the large quantum number limit.) We also generalize the definition of the volume operator in the spinfoam model to the Lorentzian signature, and show that it matches the one of loop quantum gravity, as does in the Euclidean case.Comment: 11 page

A Note on the Symmetry Reduction of SU(2) on Horizons of Various Topologies

It is known that the SU(2) degrees of freedom manifest in the description of the gravitational field in loop quantum gravity are generally reduced to U(1) degrees of freedom on an $S^2$ isolated horizon. General relativity also allows black holes with planar, toroidal, or higher genus topology for their horizons. These solutions also meet the criteria for an isolated horizon, save for the topological criterion, which is not crucial. We discuss the relevant corresponding symmetry reduction for black holes of various topologies (genus 0 and $\geq 2$) here and discuss its ramifications to black hole entropy within the loop quantum gravity paradigm. Quantities relevant to the horizon theory are calculated explicitly using a generalized ansatz for the connection and densitized triad, as well as utilizing a general metric admitting hyperbolic sub-spaces. In all scenarios, the internal symmetry may be reduced to combinations of U(1).Comment: 13 pages, two figures. Version 2 has several references updated and added, as well as some minor changes to the text. Accepted for publication in Class. Quant. Gra