The reaction of alternate oxidants with cytochrome P450BM3 and mutants generates spectrally detectable, high valent iron intermediates

"The Cytochrome P450 family serves as the forefront for xenobiotic metabolism in the human body. An understanding of the mechanism behind this enzyme family is a necessity for later research in the areas of pharmaceuticals and biochemistry. It has been observed by previous researchers that the transient species compound I, a highly reactive &#x03C0-cation radical intermediate, is able to be viewed with P450cam and other heme containing proteins with subjection to various oxidant compounds such as m-CPBA or peracetic acid. In this study Cytochrome P450BM3 and its' heme domain, P450BMH, has been reacted with various oxidant compounds to show evidence of this compound I intermediate by diode array spectrophotometry and electron paramagnetic resonance spectroscopy."--Abstract from author supplied metadata

Generating a theoretical base for restructuring curriculum content

The purpose of this study is to contribute to an ongoing effort among a number of contemporary curriculum theorists to generate a theoretical base for restructuring' curriculum content. The study is divided into a Prologue, two Chapters, and an Epilogue. PROLOGUE confronts the significant metatheoretical cal problems that are are raised simply by the articulation of this project. In the course of asking "What kind of research strategies, consonant with this intention, are appropriate for educators in North America?" the study reviews the metatheoretical considerations that have led contemporary curriculum theorists to frame such a question. Two avenues of approach that have emerged from such considerations are identified--a personal change position that involves a restructuring of individual consciousness, and a social change position that is oriented towards a restructuring of the ensemble of social relationships. The potentials of these two strategies are explored, and an argument is made for the development of a way of speaking and acting that honors the significance of both

1954: Abilene Christian College Bible Lectures - Full Text

Preface The 1954 Abilene Christian College Lectureship was one of the best attended and most successful in the history of the school. Considerable interest was manifested in the timely theme, “Overcoming Dangerous Tendencies,” and in the two special topics, “Ways and Means of Doing Mission Work,” and “Caring For Widows and Orphans.” The reports from the mission fields were highly stimulating, and all in all, the speeches were unusually high caliber. The Panel Discussions were also on timely subjects and well presented. They received a warm response, as did also the thirty classes that were conducted each day. These classes were taught by persons expert in their particular fields, and covered a wide range of interests to the faithful, working Christian. We at Abilene Christian College predict for this book of Lectures a wide and hearty reception, and believe that its reading will issue in profit to the individual and to the church at large. J. D. Thomas Lectureship Directo

1964: Abilene Christian College Bible Lectures - Full Text

LEADERSHIP IN THE CHURCH” Being the Abilene Christian College Annual Bible Lectures 1964 Price: $3.95 Published by ABILENE CHRISTIAN COLLEGE STUDENTS EXCHANGE ACC Station Abilene, Texa

Spectroscopic investigations of intermediates in the reaction of cytochrome P450BM3–F87G with surrogate oxygen atom donors

Rapid mixing of substrate-free ferric cytochrome P450BM3–F87G with m-chloroperoxybenzoic acid (mCPBA) resulted in the sequential formation of two high-valent intermediates. The first was spectrally similar to compound I species reported previously for P450CAM and CYP 119 using mCPBA as an oxidant, and it featured a low intensity Soret absorption band characterized by shoulder at 370 nm. This is the first direct observation of a P450 compound I intermediate in a type II P450 enzyme. The second intermediate, which was much more stable at pH values below 7.0, was characterized by an intense Soret absorption peak at 406 nm, similar to that seen with P450CAM [T. Spolitak, J.H. Dawson, D.P. Ballou, J. Biol. Chem. 280 (2005) 20300–20309]. Double mixing experiments in which NADPH was added to the transient 406 nm-absorbing intermediate resulted in rapid regeneration of the resting ferric state, with the flavins of the flavoprotein domain in their reduced state. EPR results were consistent with this stable intermediate species being a cytochrome c peroxidase compound ES-like species containing a protein-based radical, likely localized on a nearby Trp or Tyr residue in the active site. Iodosobenzene, peracetic acid, and sodium m-periodate also generated the intermediate at 406 nm, but not the 370 nm intermediate, indicating a probable kinetic barrier to accumulating compound I in reactions with these oxidants. The P450 ES intermediate has not been previously reported using iodosobenzene or m-periodate as the oxygen donor