Sensory quality of pilot-scale prebiotic chocolates in Malaysia

Prebiotic, such as inulin, has been applied as functional food and known for its various health benefit effects such as increased mineral absorption, improved immune response and colorectal cancer prevention. An attempt was made to determine the level of Malaysian consumers’ acceptance and market potential of newly developed pilot scale prebiotic milk chocolates (MC-1) and dark chocolates (DC-1) with no sugar added as compared to control milk and dark chocolate (containing sugar), MC-0 and DC-0, respectively. Trained panels were employed to evaluate the sensory quality of the chocolate products using the quantitative descriptive analysis (QDA) technique and the 7-point hedonic scale was used by consumer panels. In comparison with control milk chocolate (MC-0), consumer and trained panels gave better (p0.05) as control chocolates (MC-0 and DC-0). Similar likeness were shown by both trained panels and untrained Malaysian public consumers, where prebiotic milk chocolate MC-1 was the most preferred compare to prebiotic dark chocolate DC-1. This could be due to bitter taste driven by high cocoa liquor content in dark chocolate compared to milk chocolate. However, both type of prebiotic chocolates have high potential (>70%) to be bought by Malaysian consumers once introduced in the market

PROTECTIVE AND ANTIDIABETIC EFFECTS OF VIRGIN COCONUT OIL (VCO) ON BLOOD GLUCOSE CONCENTRATIONS IN ALLOXAN INDUCED DIABETIC RATS

Objective: The present study was carried out to evaluate if Virgin Coconut Oil (VCO) exerts any protective or regenerative effects on pancreatic β cells in Alloxan induced diabetic rats and to observe and determine the morphology of islets of animals from different treatment groups.Methods: Twenty six adults male Wistar rats were divided into 4 groups. Group 1 served as a control and received no treatment. Group 2 served as a diabetic control where they received 120 mg/kg body weight Alloxan at week 1. Group 3 was administered with Alloxan at 120 mg/kg body weight followed by VCO treatment for 10 w. Group 5 was administered with 0.8 ml VCO for 13 w prior to being administered with Alloxan 8 w after VCO treatment.Blood glucose readings and body weight were monitored weekly during the treatment period. IPGTT was carried out prior to animal sacrifice. Histological analysis was carried out on the pancreas of animals in VCO treated and control groups.Results: VCO treated rats were able to reduce hyperglycaemia in diabetic individuals. Histological analysis indicates that VCO was able to regenerate damaged islets of diabetic rats and was also able to prolong their survival despite being hyperglycaemic.Conclusion: VCO has potential protective and regenerative effects towards diabetic rats and further research should be evaluated to develop a probable mechanism of action.Â

Effect of extraction methods on yield, oxidative value, phytosterols and antioxidant content of cocoa butter

Cocoa beans are rich in numbers of beneficial bioactive compounds such as phenolics and phytosterols, which benefits to human being. The suitable extraction method is needed to produce high quality and quantity of cocoa butter and other bioactive compounds. There are many extraction method to extract these compounds such as Soxhlet extraction, supercritical fluid extraction, ultrasound extraction method and others. The objective of this study is to determine the effectiveness of the different extraction methods producing high yields of cocoa butter, lower oxidative value, stable phytosterols and antioxidant content. The cocoa beans were subjected to different extraction methods such as Soxhlet extraction (SE), Ultrasonic extraction method (USE), Supercritical carbon dioxide (SCO2) and Supercritical carbon dioxide with co-solvent (SCO2-Ethanol). Cocoa butter extracted using SCO2-Ethanol has significantly (p<0.05) obtained highest cocoa butter yield (37.05%) and phytosterols content (6441 μg/g of extract) compared to SE (28.87% and 4960 μg/g of extract), SCO2 (31.32% and 5492 μg/g of extract) and USE (34.81% and 5106 μg/g of extract). Meanwhile, the oxidative value of SCO2-Ethanol was significantly (p<0.05) obtained lowest value compared to other extraction methods. Extraction method are crucial in cocoa industry to minimise the cost during processing, obtain maximum extraction yield and preserve the bioactive compounds thus will improve the value of cocoa butter improve the value of cocoa butter

Effects of Candida sp. and Blastobotrys sp. starter on fermentation of cocoa (Theobroma cacao L.) beans and its antibacterial activity

The effects of Candida sp. and Blastobotrys sp. starters on fermentation of cocoa beans were studied to determine the potential antibacterial activity of its extracts against foodborne pathogens. The fermentations of cacao beans using Candida sp. and Blastobotrys sp. were conducted for seven days. The observed parameters including pH and temperature monitoring during fermentation, detected active compounds, and invitro antibacterial activity against several foodborne pathogens at on sampling day 0, 3 and 7. Spontaneous fermentation (without starter culture added) was used as control. The pH during fermentation increased from pH 3.00 to 7.97, pH 3.00 to 7.68, and pH 3.00 to 7.54 for spontaneous, Candida sp. and Blastobotrys sp. fermentation respectively. The temperatures of fermentation ranged from 28oC to 33oC, 28oC to 32oC, and 28oC to 32oC for fermentation by spontaneous, Candida sp. and Blastobotrys sp., respectively. Gas Chromatography Mass Spectrometry (GC-MS) analyses showed several active compounds including caffeine, theobromine, gamma-tocopherol, stigmasterol and beta-sitosterols in all three fermentations. Caffeine content was the highest (74.59%) in control fermentation in earlier process. Theobromine content was higher for control fermentation compared to other Candida sp. and Blastobotrys sp. fermentation. Generally, gamma-tocopherol, stigmasterol and beta-sitosterols contents declined in the middle of the fermentation period but increased again towards the end. Fermented cocoa beans extract exhibited antibacterial activity against Bacillus cereus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Salmonella enteritica, and Staphylococcus aureus. However, the extracts did not show any antibacterial activity against Listeria monocytogenes and Pseudomonas aeruginosa. In summary, the addition of starter cultures namely Candida sp. and Blastobotrys sp. in fermentation of cocoa beans able to trigger off the active compounds and show potent antibacterial activity against several foodborne pathogens

Preliminary study of in vitro propagation of a selected high yielding clone of kacip fatimah (Labisia pumila VAR. alata)

Kacip Fatimah (Labisia pumila), an undershrub herb from the Myrsinaceae family, is traditionally used as medicine, particularly by Malay women during post-natal care. The development of L. pumila as a medicinal herb has led to its commercialization and increasing demand. To ensure its sustainable supply, the propagation of kacip Fatimah on a large scale such as through tissue culture needs to be explored. In this study, the leaves of a selected high yielding clone of kacip Fatimah, clone AA28, were used as explants. After surface sterilization, the explants were cultured on MS basal medium containing 0.5 mg/l NAA with different concentrations of BAP ranging from 0.05 to 0.15 mg/l for shoot induction. The explants cultured in MS medium containing 0.5 mg/l NAA in combination with 0.05 mg/l BAP produced the highest mean number of shoots per explants (10 ± 0.1). The shoot multiplication study is in progress and will be reported in a future paper

POTENTIAL EFFECTS OF DUKU (LANSIUM DOMESTICUM CORR) AND LANGSAT (LANSIUM DOMESTICUM JACK) EXTRACTS ON THE GROWTH OF BIFIDOBACTERIA SPP.

Objective: Lansium domesticum Corr. is a fruit tree species belongs to the family Meliaceae. There are numerous forms of the species and grouped into two main types: Duku and Langsat. The objective of this study is to screen the ability of adding extracts of freeze-dried duku and langsat to stimulate the growth and stability of selected Bifidobacteria spp in skimmed milk.Methods: Samples were prepared by adding either 5% or 12% of oligosaccharides from duku, langsat, inulin, galacto-oligosaccharides (GOS) and fructo-oligosaccharides (FOS) to 5% and 12% (w/v) reconstituted nonfat dry milk (NDM), respectively. The specific growth rates (µ) for each sample were calculated. All experiments were replicated ten times.Results: The mean doubling time (Td) for Bifidobacterium longum, was lowest in the presence of freeze-dried duku and langsat compared to GOS, FOS and inulin. Retention of the viability of five Bifidobacterium species was greatest in the presence of freeze-dried duku and langsat followed by GOS, FOS and inulin. The highest percentage of acetic and lactic acids were produced by B. longum, B. infantis and B. adolescentis with freeze-dried duku and langsat. The pattern of results was similar to the commercial product, oligosaccharides (inulin, GOS and FOS). Conclusion: Therefore, this study provides promising results on promoting growth and probiotic activity of natural oligosaccharides compound from freeze-dried duku and langsat

Role of the employment status and education of mothers in the prevalence of intestinal parasitic infections in Mexican rural schoolchildren

&lt;p&gt;&lt;b&gt;Background:&lt;/b&gt; Intestinal parasitic infections are a public health problem in developing countries such as Mexico. As a result, two governmental programmes have been implemented: a) "National Deworming Campaign" and b) "Opportunities" aimed at maternal care. However, both programmes are developed separately and their impact is still unknown. We independently investigated whether a variety of socio-economic factors, including maternal education and employment levels, were associated with intestinal parasite infection in rural school children.&lt;/p&gt; &lt;p&gt;&lt;b&gt;Methods:&lt;/b&gt; This cross-sectional study was conducted in 12 rural communities in two Mexican states. The study sites and populations were selected on the basis of the following traits: a) presence of activities by the national administration of albendazole, b) high rates of intestinal parasitism, c) little access to medical examination, and d) a population having less than 2,500 inhabitants. A total of 507 schoolchildren (mean age 8.2 years) were recruited and 1,521 stool samples collected (3 per child). Socio-economic information was obtained by an oral questionnaire. Regression modelling was used to determine the association of socio-economic indicators and intestinal parasitism.&lt;/p&gt; &lt;p&gt;&lt;b&gt;Results:&lt;/b&gt; More than half of the schoolchildren showed poliparasitism (52%) and protozoan infections (65%). The prevalence of helminth infections was higher in children from Oaxaca (53%) than in those from Sinaloa (33%) (p &#60; 0.0001). Giardia duodenalis and Hymenolepis nana showed a high prevalence in both states. Ascaris lumbricoides, Trichuris trichiura and Entamoeba hystolitica/dispar showed low prevalence. Children from lower-income families and with unemployed and less educated mothers showed higher risk of intestinal parasitism (odds ratio (OR) 6.0, 95% confidence interval (CI) 1.6–22.6; OR 4.5, 95% CI 2.5–8.2; OR 3.3, 95% CI 1.5–7.4 respectively). Defecation in open areas was also a high risk factor for infection (OR 2.4, 95% CI 2.0–3.0).&lt;/p&gt; &lt;p&gt;&lt;b&gt;Conclusion:&lt;/b&gt; Intestinal parasitism remains an important public health problem in Sinaloa (north-western Mexico) and Oaxaca (south-eastern Mexico). Lower income, defecation in open areas, employment status and a lower education level of mothers were the significant factors related to these infections. We conclude that mothers should be involved in health initiatives to control intestinal parasitism in Mexico.&lt;/p&gt

Effect of Thickness on the Photocatalytic Performance of ZnO/SBR Composites

Photocatalyst of zinc oxide/styrene butadiene rubber (ZnO/SBR) composite with 80/20 (wt./wt.) concentration were prepared by conventional rubber processing technique. The composite was mixed using two-roll mills at 27 °C for 10 min and compressed into a flat sheet with a thickness ranging from 0.15 to 1.08 mm. The characteristics of photocatalyst were studied by Fourier-transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-Vis). The photocatalyst activity of ZnO/SBR was evaluated using methylene blue (MB) as a pollutant under visible light. The ZnO/SBR with 0.54 mm thickness showed the highest performance of photodegradation of MB compared to other thicknesses. This is due to the sheet buoyancy increasing the light-capturing ability

Relationship between blood lead concentration and nutritional status among Malay primary school children in Kuala Lumpur, Malaysia.

A cross-sectional study was conducted to identify the relationship between blood lead concentration and nutritional status among primary school children in Kuala Lumpur. A total of 225 Malay students, 113 male and 112 female, aged 6.3 to 9.8 were selected through a stratified random sampling method. The random blood samples were collected and blood lead concentration was measured by a Graphite Furnace Atomic Absorption Spectrophotometer. The nutrient intake was determined by the 24-hour Dietary Recall method and Food Frequency Questionnaire. An anthropometric assessment was reported according to growth indices (z-scores of weight-for-age, height-for-age, and weight-for-height). The mean blood lead concentration was low (3.4 ± 1.91 ug/dL) and was significantly different between gender. Only 14.7% of the respondents fulfilled the daily energy requirement. The protein and iron intakes were adequate for a majority of the children. However, 34.7% of the total children showed inadequate intake of calcium. The energy, protein, fat and carbohydrate intakes were significantly different by gender, that is, males had better intake than females. Majority of respondents had normal mean z-score of growth indices. Ten percent of the respondents were underweight, 2.8% wasted and 5.4% stunted. Multiple linear regression showed inverse significant relationships between blood lead concentration with children's age (β= -0.647, p<0.001) and per capita income (β=-0.001, p=0.018). There were inverse significant relationships between blood lead concentration with children's age (β=-0.877, p=0.001) and calcium intake (β= -0.011,p=0.014) and positive significant relationship with weight-for-height (β=0.326, p=0.041) among those with inadequate calcium intake. Among children with inadequate energy intake, children's age (β= -0.621, p< 0.001), per capita income (β= -0.001,p=0.025) and protein intake (β= -0.019, p=0.027) were inversely and significantly related with blood lead concentration. In conclusion, nutritional status might affect the children's absorption of lead and further investigation is required for confirmation