Validation of Humanized Mouse Antibodies

Antibody therapy is being developed and tested as one of the most promising agents for treatment of various human diseases. As of March 2016, 350 antibody candidates are in clinical trials. Many of these antibodies have been taken from animals and “humanized” by genetic modification. Our experiment tests monoclonal antibodies that have been harvested from mouse hybridoma (spleen-derived) cells and cloned until the heavy and light chains of the antibody can be recognized by human cells. Because of this “humanization” procedure, basic antibody assays are needed to demonstrate that the binding, specificity and functional parameters of the antibodies are not lost during cloning. The purpose of this research is to perform this validation through assays. The antibodies are harvested from cell supernatants and purified using affinity chromatography. Then, the antibody fractions are tested for reactivity with human target protein PTP-Beta, via western blot and ELISA procedures. Cross-reactivity of the antibody is tested against human eta and cynomolgus beta proteins. The work presented in this poster describes results from one particular mouse antibody, R15, which has been humanized to functionally enhance endothelial survival. The goal is to generate a therapeutic antibody candidate that improves endothelium survival and stability

Mapping the Planetary Wake in HD 163296 with Kinematics

We map the planetary wake associated with the embedded protoplanet creating the CO kink in the disk of HD~163296. We show that the wake can be traced by a series of correlated perturbations in the peak velocity map. The sign change of the perturbations across the disk major axis confirm that the wake induces predominantly radial motion, as predicted by models of planet-disk interaction. These results provide the first direct confirmation of planet wakes generated by Lindblad resonances. Mapping the wake provides a constraint on the disk aspect ratio, which is required to measure the mass of the planet.Comment: 10 Pages, 6 figures. Accepted in ApJ

‘Scots and Scabs from North-by-Tweed’:Undesirable Scottish Migrants in Seventeenth- and Early Eighteenth-Century England

While very prominent in the contemporary world, anxiety about the potentially negative impact that immigrants might have on their host communities has deep historical roots. In a British context, such fears were particularly heightened following the regal union of 1603 when large numbers of Scots began settling in England. This article offers a fresh perspective on these issues by exploring the experiences and reception of poor, deviant or otherwise ‘undesirable’ Scottish migrants to England during the seventeenth and eighteenth centuries. Focusing in particular on chapmen, vagrants and criminals, it suggests that, while in general Scots were able to integrate relatively easily into English society, there existed an unwelcome subset surviving by dubious means. Though not usually attracting unduly severe treatment on account of their nationality, these unwelcome migrants had a disproportionate effect on English perceptions of and attitudes towards the broader cohort of Scottish migrants in their midst

Culturally valuable minority crops provide a succession of floral resources for flower visitors in traditional orchard gardens

Agricultural intensification typically has detrimental effects on pollinator communities, but diverse cropping systems that contain sequentially-flowering crops have the potential to benefit pollinators through the provision of additional floral resources. In this study we investigate the importance of cultivated flora for flower visitors in ten agricultural gardens in South Sinai, Egypt. Insect-flower interactions in gardens and unmanaged plots were surveyed across a four-month period in two environmentally distinct years (pre-flood and post-flood). Despite containing an equal abundance and diversity of wild plants as unmanaged habitat, gardens supported a higher abundance and diversity of flower visitors due to the additional presence of cultivated flora. Visitation networks exhibited dramatic intra-annual changes in composition, with cultivated plants becoming increasingly important in later months. Trends were highly conserved across 2 years despite highly contrasting rainfall. Several key crop species were strongly involved in shaping the structure of the networks, the majority of which were herbs with strong cultural significance (fennel, rosemary, mint) and grown incidentally alongside the primary orchard crops. Minority crops are frequently overlooked in agricultural systems due to their low economic value, but we show that they can have a dramatic influence upon the structure of visitation networks, increasing both pollinator abundance and diversity, and emphasising the link between cultural practices and biodiversity conservation

Kinematic and thermal signatures of the directly imaged protoplanet candidate around Elias 2-24

We report kinematic and thermal signatures associated with the directly imaged protoplanet candidate in the Elias 2-24 disc. Using the DSHARP ALMA observations of the $^{12}$CO J=2-1 line, we show that the disc kinematics are perturbed, with a detached CO emission spot at the location of the planet candidate and traces of spiral wakes, and also that the observed CO emission intensities require local heating. While the foreground extinction hides the velocity channels associated with the planet, preventing a planet mass estimate, the level of gas heating implied by the CO emission indicates the presence of a warm, embedded giant planet. Comparison with models show this could either be a $\gtrsim 5$M$_\mathrm{Jup}$, or a lower mass ( $\gtrsim 2$M$_\mathrm{Jup}$) but accreting proto-planet.Comment: 6 pages, 3 figures, accepted for publication in MNRAS Letter