Impact of chronic stress protocols in learning and memory in rodents: systematic review and meta-analysis

The idea that maladaptive stress impairs cognitive function has been a cornerstone of decades in basic and clinical research. However, disparate findings have reinforced the need to aggregate results from multiple sources in order to confirm the validity of such statement. In this work, a systematic review and meta-analyses were performed to aggregate results from rodent studies investigating the impact of chronic stress on learning and memory. Results obtained from the included studies revealed a significant effect of stress on global cognitive performance. In addition, stressed rodents presented worse consolidation of learned memories, although no significantly differences between groups at the acquisition phase were found. Despite the methodological heterogeneity across studies, these effects were independent of the type of stress, animals' strains or age. However, our findings suggest that stress yields a more detrimental effect on spatial navigation tests' performance. Surprisingly, the vast majority of the selected studies in this field did not report appropriate statistics and were excluded from the quantitative analysis. We have therefore purposed a set of guidelines termed PROBE (Preferred Reporting Orientations for Behavioral Experiments) to promote an adequate reporting of behavioral experiments.This work was funded by the European Commission (FP7) "SwitchBox" (Contract HEALTH-F2-2010-259772) project and co-financed by the Portuguese North Regional Operational Program (ON.2 - O Novo Norte) under the National Strategic Reference Framework (QREN), through the European Regional Development Fund (FEDER), and by Fundacao Calouste Gulbenkian (Portugal) (Contract grant number: P-139977; project "Better mental health during ageing based on temporal prediction of individual brain ageing trajectories (TEMPO)"). PSM is supported by an FCT fellowship grant, from the PhD-iHES program, with the reference PDE/BDE/113601/2015.info:eu-repo/semantics/publishedVersio

The effect of the type of composite resin and debonding plier on the adhesive remnants on the enamel surface; A new method in adhesive remnant measuring

"nBackground and Aim: At the end of fixed orthodontic treatment, enamel damages are affected by amount of adhesive remnants. Thus, determining the factors involves in the amount of adhesive remnants e.g. the types of resins and the methods used for debonding is of necessity. The aim of this study was to evaluate the effect of the composite resin type and debonding pliers on enamel surface by a new method in measuring the adhesive remnant. "nMaterials and Methods: In this interventionally in-vitro study, 120 extracted human premolars were randomly divided into four groups of 30 each. The standard edgewise metal brackets were bonded on the teeth with two pastes self-cure composite "Concise" in the first and second groups, and with No-mix composite "Unite" in the third and fourth groups. the debonding was done by means of Lift off Debonding Instrument "LODI" (3M-Unitek), in first and third groups, and Bracket Removing Plier (Dentaurum), in the second and forth groups, respectively. After debonding, all teeth were evaluated with steriology software (stereolith version 1) for comparing the amount of adhesive remnants between the four groups. For data analysis, One-way ANOVA and independent t.test were used. "nResults: The fourth group showed the lowest adhesive on the enamel surface and the most on the bracket surface with significant differences with other three groups (p<0.01). "nConclusion: The use of Dentaurum debonding plier with shear-peel force when used with the Unite adhesive (group 4), would decrease the adhesive remnants on the enamel surface

Ameliorative effect of curcumin on lead‐induced hematological and hepatorenal toxicity in a rat model

Introduction: Lead (Pb) is a ubiquitous toxic heavy metal that inflicts numerous clinical consequences on humans. Curcumin is the principal component of turmeric, which is reported to have antioxidative properties. This study aimed at evaluating the ameliorative effects of curcumin on Pb-induced hepatorenal toxicity in a rat model. Methods: Thirty-six male Sprague-Dawley rats were randomly assigned into five groups with 12 rats in the control (normal saline) and six rats each for the lead-treated group (LTG) (50 mg/kg lead acetate [Pb acetate] for 4 weeks), recovery group (50 mg/kg Pb acetate for 4 weeks and left with no treatment for another 4 weeks), treatment group 1 (Cur100) (50 mg/kg Pb acetate for 4 weeks, followed by 100 mg/kg curcumin for 4 weeks), and treatment group 2 (Cur200) (50 mg/kg Pb acetate for 4 weeks, followed by 200 mg/kg curcumin for 4 weeks). All the experimental groups received oral treatments via orogastric-tube on alternate days. Pb concentration in the liver and kidney of the rats were evaluated using inductive-coupled plasma mass spectrometry techniques. Results: Pb-administered rats revealed significant alteration in oxidative status and increased Pb concentration in their liver and kidney with obvious reduction of hemogram and increased in leukogram as well as aberration in histological architecture of the liver and kidney. However, treatment with curcumin reduces the tissue Pb concentrations and ameliorates the above mention alterations. Conclusions: The results in this study suggested that curcumin attenuates Pb-induced hepatorenal toxicity via chelating activity and inhibition of oxidative stress