Evaluation of biochemical markers (sugar, proline, malonedialdehyde and ethylene) for cold sensitivity in microcuttings of two coffee species

Des microboutures de caféiers (#Coffea arabica L. et #Coffea canephora Pierre) ont été conservées #in vitro pendant 0-20 semaines à différentes températures (12, 17, 22 et 27°C). La survie est généralement élevée, sauf pour #C. canephora conservée à 12°C. Après une augmentation transitoire de la concentration en glucose et fructose au début de la période de stockage, le niveau de sucre reste stable. Pendant les premières semaines de conservation, la quantité de proline est multipliée par deux pour les deux espèces, à 12°C. Une chute de la concentration en proline est observée chez #C. canephora à la fin de la période de conservation à 12°C. Alors que chez #C. arabica, aucune modification n'est notée dans la concentration en malonedialdéhyde (MDA), son niveau augmente dans les boutures de #C. canephora stockées à 12°C. Cette augmentation est réversible après 10 jours à 27°C, au début de la période de stockage, mais elle devient irréversible après 20 semaines de conservation. La production d'éthylène démarre après transfert à 27°C dès deux semaines de conservation pour #C. canephora, mais après 12 semaines seulement pour #C. arabica. Cette synthèse diminue à la fin de la période de stockage chez les boutures de #C. canephora stockées à 12°C. Les résultats indiquent que parmi les quatre composés utilisés, le MDA apparaît comme le meilleur indicateur de la sensibilité au froid chez les deux espèces de caféier étudiées. (Résumé d'auteur

Effets comparés du vieillissement naturel et accéléré sur les semences d'orge (Hordeum vulgare L.)

Les effets d'un vieillissement accéléré (VA) sont étudiés avec des semences d'orge (#Hordeum vulgare$ L.) et comparés à ceux d'un vieillissement naturel au cours d'un stockage à 4°C depuis une trentaine d'années. Le vieillissement accéléré (42°C et 100% d'humidité relative) se manifeste, après 4 jours de traitement, par une diminution de la vitesse et du taux de germination, un ralentissement de la croissance des plantules et l'apparition de plantules anormales. Les semences de sept variétés, conservées depuis trente ans à 4°C et 30% d'humidité relative, ont gardé une très bonne aptitude à la germination (supérieure à 95%). Seule, la variété Atlas à grain nu a perdu 30% de faculté germinative et réagit le plus au VA. Le vieillissement accéléré n'entraîne aucune modification biochimique qualitative et quantitative des isozymes de peroxydases. En revanche, les zymogrammes de peroxydase des semences conservées depuis trente ans sont différents de ceux des semences récentes. Pour certaines variétés, un électromorphe s'exprime avec une intensité plus faible et disparaît pour les autres variétés. L'utilité des tests de VA comme prédicteurs de la capacité à la conservation est discutée. (Résumé d'auteur

A complementary approach to estimate the internal pressure of fission gas bubbles by SEM-SIMS-EPMA in irradiated nuclear fuels

International audienceThe behaviour of gases produced by fission is of great importance for nuclear fuel in operation. Within this context, a decade ago, a general method for the characterisation of the fission gas including gas bubbles in an irradiated UO$_2$ nuclear fuel was developed and applied to determine the bubbles internal pressure. The method consists in the determination of the pressure, over a large population of bubbles, using three techniques: SEM, EPMA and SIMS. In this paper, a complementary approach using the information given by the same techniques is performed on an isolated bubble under the surface and is aiming for a better accuracy compared to the more general measurement of gas content. SEM and EPMA enable the detection of a bubble filled with xenon under the surface. SIMS enables the detection of the gas filling the bubble. The quantification is achieved using the EPMA data as reference at positions where no or nearly no bubbles are detected

Annual transcriptome of a key zooplankton species, the copepod Calanus finmarchicus

The copepod Calanus finmarchicus (Crustacea, Copepoda) is a key zooplanktonic species with a crucial position in the North Atlantic food web and significant contributor to ocean carbon flux. Like many other high latitude animals, it has evolved a programmed arrested development called diapause to cope with long periods of limited food supply, while growth and reproduction are timed to take advantage of seasonal peaks in primary production. However, anthropogenic warming is inducing changes in the expected timing of phytoplankton blooms, suggesting phenological mismatches with negative consequences for the N. Atlantic ecosystem. While diapause mechanisms are mainly studied in terrestrial arthropods, specifically on laboratory model species, such as the fruit fly Drosophila, the molecular investigations of annual rhythms in wild marine species remain fragmentary. Here we performed a rigorous year-long monthly sampling campaign of C. finmarchicus in a Scottish Loch (UK; 56.45°N, 5.18°W) to generate an annual transcriptome. The mRNA of 36 samples (monthly triplicate of 25 individuals) have been deeply sequenced with an average depth of 137 ± 4 million reads (mean ± SE) per sample, aligned to the reference transcriptome, and filtered. We detail the quality assessment of the datasets and provide a high-quality resource for the investigation of wild annual transcriptomic rhythms (35,357 components) in a key diapausing zooplanktonic species

The conserved C-terminus of the PcrA/UvrD helicase interacts directly with RNA polymerase

Copyright: © 2013 Gwynn et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was supported by a Wellcome Trust project grant to MD (Reference: 077368), an ERC starting grant to MD (Acronym: SM-DNA-REPAIR) and a BBSRC project grant to PM, NS and MD (Reference: BB/I003142/1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD

A First Look at Spatially Resolved Balmer Decrements at 1.0<z<2.41.0<z<2.4 from JWST NIRISS Slitless Spectroscopy

We present the first results on the spatial distribution of dust attenuation at $1.0<z<2.4$ traced by the Balmer Decrement, H$\alpha$/H$\beta$, in emission-line galaxies using deep JWST NIRISS slitless spectroscopy from the CAnadian NIRISS Unbiased Cluster Survey (CANUCS). H$\alpha$ and H$\beta$ emission line maps of emission-line galaxies are extracted and stacked in bins of stellar mass for two grism redshift bins, $1.0<z_{grism}<1.7$ and $1.7<z_{grism}<2.4$. Surface brightness profiles for the Balmer Decrement are measured and radial profiles of the dust attenuation towards H$\alpha$, $A_{\mathrm{H}\alpha}$, are derived. In both redshift bins, the integrated Balmer Decrement increases with stellar mass. Lower mass ($7.6\leqslant$Log($M_{*}$/M$_{\odot}$)$<10.0$) galaxies have centrally concentrated, negative dust attenuation profiles whereas higher mass galaxies ($10.0\leqslant$Log($M_{*}$/M$_{\odot}$)$<11.1$) have flat dust attenuation profiles. The total dust obscuration is mild, with on average $0.07\pm0.07$ and $0.14\pm0.07$ mag in the low and high redshift bins respectively. We model the typical light profiles of star-forming galaxies at these redshifts and stellar masses with GALFIT and apply both uniform and radially varying dust attenuation corrections based on our integrated Balmer Decrements and radial dust attenuation profiles. If these galaxies were observed with typical JWST NIRSpec slit spectroscopy ($0.2\times0.5^{\prime\prime}$ shutters), on average, H$\alpha$ star formation rates (SFRs) measured after slit-loss corrections assuming uniform dust attenuation will overestimate the total SFR by $6\pm21 \%$ and $26\pm9 \%$ at $1.0\leqslant z < 1.7$ and $1.7\leqslant z < 2.4$ respectively.Comment: 7 pages, 5 figures, submitted to ApJ

DNA cruciform arms nucleate through a correlated but non-synchronous cooperative mechanism

Inverted repeat (IR) sequences in DNA can form non-canonical cruciform structures to relieve torsional stress. We use Monte Carlo simulations of a recently developed coarse-grained model of DNA to demonstrate that the nucleation of a cruciform can proceed through a cooperative mechanism. Firstly, a twist-induced denaturation bubble must diffuse so that its midpoint is near the centre of symmetry of the IR sequence. Secondly, bubble fluctuations must be large enough to allow one of the arms to form a small number of hairpin bonds. Once the first arm is partially formed, the second arm can rapidly grow to a similar size. Because bubbles can twist back on themselves, they need considerably fewer bases to resolve torsional stress than the final cruciform state does. The initially stabilised cruciform therefore continues to grow, which typically proceeds synchronously, reminiscent of the S-type mechanism of cruciform formation. By using umbrella sampling techniques we calculate, for different temperatures and superhelical densities, the free energy as a function of the number of bonds in each cruciform along the correlated but non-synchronous nucleation pathways we observed in direct simulations.Comment: 12 pages main paper + 11 pages supplementary dat

The near-infrared imager and slitless spectrograph for the James Webb Space Telescope. II. Wide field slitless spectroscopy

We acknowledge support from the Natural Sciences and Engineering Research Council of Canada (NSERC) through a variety of its funding programs. This work is in part supported by Canadian Space Agency grant 18JWSTGTO1. C.P., S.R. and K.V. are supported by the Canadian Space Agency under a contract with NRC Herzberg Astronomy and Astrophysics. K.M. acknowledges funding by the Science and Technology Foundation of Portugal (FCT), grants No. IF/00194/2015, PTDC/FIS-AST/28731/2017, UIDB/00099/2020.We present the wide field slitless spectroscopy mode of the NIRISS instrument on the James Webb Space Telescope. This mode employs two orthogonal low-resolution (resolving power ≈150) grisms in combination with a set of six blocking filters in the wavelength range 0.8–2.3 μm to provide a spectrum of almost every source across the field-of-view. When combined with the low background, high sensitivity and high spatial resolution afforded by the telescope, this mode will enable unprecedented studies of the structure and evolution of distant galaxies. We describe the performance of the as-built hardware relevant to this mode and expected imaging and spectroscopic sensitivity. We discuss operational and calibration procedures to obtain the highest quality data. As examples of the observing mode usage, we present details of two planned Guaranteed Time Observations programs: The Canadian NIRISS Unbiased Cluster Survey and The NIRISS Survey for Young Brown Dwarfs and Rogue Planets.Publisher PDFPeer reviewe

Spring temperature responses of oaks are synchronous with North Atlantic conditions during the last deglaciation

Paleoclimate proxies based on the measurement of xylem cell anatomy have rarely been developed across the temperature range of a species or applied to wood predating the most recent millennium. Here we describe wood anatomy-based proxies for spring temperatures in central North America from modern bur oaks (Quercus macrocarpa Michx.). The strong coherence of temperature signals across the species range supports the use of these proxies across thousands of years of climatic change. We also used 79 subfossil oak log cross sections from northern Missouri, ¹⁴C-dated to 9.9-13.63 ka (ka is 1000 cal yr BP), to assess the frequency of oak deposition into alluvial sediments and a subset of these oaks for a wood anatomy-based reconstruction of spring paleotemperatures. Temperatures during the Younger Dryas cold period (YD) were up to 3.5 degrees C lower than modern temperatures for that region, equivalent to or lower than those experienced at the northern edge of the modern species range. Compared to extant oaks growing at much higher [CO₂], subfossil oaks had greater vessel frequencies. Besides very low theoretical (or estimated) xylem conductivity near the beginning of the oak record near 13.6 ka, vessel frequencies greater than modern trees compensated for reduced vessel dimensions so that theoretical xylem conductivity was consistently above that of modern trees at the cold northern sites. Significant correlations were found between the frequency of ¹⁴C-dated oaks and either delta δ¹⁸O from the NGRIP (North Greenland Ice Core Project) ice core or from the Cariaco grayscale marine-sediment record from the southern Caribbean sea. Oak deposition into alluvial sediments during the YD was significantly lower than expected given the average sample depth of oaks from 9.9 to 13.6 ka. Reduced oak deposition during the YD suggests that an abrupt shift in climate reduced oak populations across the region and/or changed the rates of channel movement across drainages.Keywords: Pleistocene, Quercus macrocarpa, Holocene, Pre-Boreal, Younger Dryas, Radiocarbon, Wood anatomy, Great Plains\, USA, Phenology, Bolling-Allerod, Xylem, Bur oa