The relationship between thigh and leg circumferences and proprioceptive sense: results of postoperative anterior cruciate ligament reconstruction

Proprioceptive sense is damaged in anterior cruciate ligament (ACL) injuries and restoring joint stabilization is very important. The aim of the present study is to evaluate the relationship between the post-operative thigh and leg circumferences and proprioceptive sense on both operated and non-operated sides in patients who have undergone Semitendinosus/Gracilis ST/G (Hamstring Autograft) anterior cruciate ligament reconstruction (ACLR). 20 sedentary male patients aged 18-35 years who had undergone ST/G ACLR at least 6 months ago were included in the study. Leg and thigh circumferences and proprioceptive sense measurements were performed on the operated (OP) and non-operative (NONOP) sides of the patients. Leg and thigh circumferences were recorded in cm with a flexible meter. Proprioceptive sense measurements were determined at 45, 60 and 75 angles for extension (Ex) and 105, 120, 135 angles for flexion (Flx). When the findings of the patients were evaluated statistically between the operated and non-operated sides, no significant difference was found in the leg (p=0.070), thigh (p=0.403) circumferences and proprioception (Ex 45o p=0.547, Ex 60o p=0.124, Ex 75o p=0.956) (Flx 105o p=0.663, Flx 120o p=0.837, Flx 135o p=0.546). No general significance was found in the correlation analyses evaluated on both the operated and non-operated sides. There was a significant positive correlation between thigh circumference and Flx120º in terms of proprioception only on the non-operated side (p [Med-Science 2023; 12(2.000): 587-92

The Relationship between Lower Extremity Functional Performance and Balance after Anterior Cruciate Ligament Reconstruction: Results of Patients Treated with the Modified All-Inside Technique

Background and Objectives: Anterior cruciate ligament (ACL) ruptures are common injuries, and ACL reconstruction (ACLR) is among the most common surgical procedures in sports surgery. Our research aims to compare the 6-month post-operative results of the modified all-inside (MAI) ACLR technique, single leg hop tests (SLHT), and Y balance tests applied in different directions on the operated and non-operated sides. Materials and Methods: A retrospective cohort of 22 male recreational athletes who underwent MAI ACLR techniques performed by the same surgeon were evaluated. The functional knee strengths of the participants on the operated and non-operated sides were evaluated with five different tests of SLHTs: single hop for distance (SH), triple hop for distance (TH), crossover triple hop for distance (CH), medial side triple hop for distance (MSTH), and medial rotation (90 degrees) with hop for distance (MRH). Their dynamic balance was evaluated with the Y balance Test. Results: Compared to pre-operative levels, there was a significant improvement in the mean Lysholm, Tegner, and IKDC scores during the post-operative period (p 0.05). There were no significant relationships between YBT (composite scores) and SH, TH, CH, MSTH, and MRH distances in the healthy leg (p > 0.05), but a significant correlation with only CH in the ACL leg (p < 0.05). Conclusions: Our research shows that sixth-month post-operative SLHT findings were lower on the ACL side compared to the healthy side in patients tested with the MAI ACLR technique. However, when these scores are evaluated in terms of balance, it can be seen that both sides reveal similar findings. The similarity of LSIs in SLHTs applied in different directions, and balance scores of ACL and healthy sides revealed that the MAI technique is also an ACLR technique that can be used in athletes from a functional point of view

A Meta-Analysis: Identification of Common Mir-145 Target Genes that have Similar Behavior in Different GEO Datasets.

BACKGROUND:MicroRNAs, which are small regulatory RNAs, post-transcriptionally regulate gene expression by binding 3'-UTR of their mRNA targets. Their deregulation has been shown to cause increased proliferation, migration, invasion, and apoptosis. miR-145, an important tumor supressor microRNA, has shown to be downregulated in many cancer types and has crucial roles in tumor initiation, progression, metastasis, invasion, recurrence, and chemo-radioresistance. Our aim is to investigate potential common target genes of miR-145, and to help understanding the underlying molecular pathways of tumor pathogenesis in association with those common target genes. METHODS:Eight published microarray datasets, where targets of mir-145 were investigated in cell lines upon mir-145 over expression, were included into this study for meta-analysis. Inter group variabilities were assessed by box-plot analysis. Microarray datasets were analyzed using GEOquery package in Bioconducter 3.2 with R version 3.2.2 and two-way Hierarchical Clustering was used for gene expression data analysis. RESULTS:Meta-analysis of different GEO datasets showed that UNG, FUCA2, DERA, GMFB, TF, and SNX2 were commonly downregulated genes, whereas MYL9 and TAGLN were found to be commonly upregulated upon mir-145 over expression in prostate, breast, esophageal, bladder cancer, and head and neck squamous cell carcinoma. Biological process, molecular function, and pathway analysis of these potential targets of mir-145 through functional enrichments in PPI network demonstrated that those genes are significantly involved in telomere maintenance, DNA binding and repair mechanisms. CONCLUSION:As a conclusion, our results indicated that mir-145, through targeting its common potential targets, may significantly contribute to tumor pathogenesis in distinct cancer types and might serve as an important target for cancer therapy

Zinc-coated Foam With Negative Pressure Wound Therapy in the Treatment of Challenging Wounds: A New Alternative Interface Material

Objective. The aim of the study was to present the authors' clinical observations on zinc-coated foam with negative pressure wound therapy (NPWT). MaterialS and Methods. Ninety-four consecutive patients treated with zinc-coated foam with VAC therapy were retrospectively reviewed. Nonhealing wounds of at least 6 months duration with high to moderate exudate that required open wound management to secondary intervention were included in this study. The evaluation criteria consisted of the measurement of wound surface area, length Of overall treatment time, and a clinical observation of granulation tissue formation in the wound bed. Results. In all wounds, there was a significant decrease of the wound surface area, and wound exudate was obtained at the end Of the treatment. There was a statistically significant difference between pretreatment and posttreatment measurements (P < 0.05). In all wounds, granulation tissue formation was clinically observed by day 6. Of the 94 wounds, 72 were surgically closed and 22 healed secondarily. The follow-up period averaged 12 months, and it was uneventful with no sign of complications from the use of the material. Conclusion. The results of the retrospective study demonstrate zinc-coatedfoam with NPWT can be safely used as an effective and alternative interface material in the treatment of challenging wounds

Biological process (GO) of the potential targets of mir-145 by functional enrichments in PPI network.

<p>Biological process (GO) of the potential targets of mir-145 by functional enrichments in PPI network.</p

PPI network of commonly deregulated mir-145 targets.

<p>Pink: experimentally determined (known interactions), Blue: from curated databases (known interactions). Yellow: textmining, Green: gene neighborhood (Predicted interactions), Black: co-expression. The interaction score was set to high confidence (0.700).</p

A summary of the each individual microarray datasets from different GEO dataset.

<p>A summary of the each individual microarray datasets from different GEO dataset.</p

KEGG Pathways of the potential targets of mir-145 by functional enrichments in PPI network.

<p>KEGG Pathways of the potential targets of mir-145 by functional enrichments in PPI network.</p

Principal component analysis (PCA) plot for combined dataset before (left panel) and after (right panel) removing batch effect.

<p>Principal component analysis (PCA) plot for combined dataset before (left panel) and after (right panel) removing batch effect.</p

Flow chart of study selection in the meta-analysis.

<p>Flow chart of study selection in the meta-analysis.</p