Warp propagation in astrophysical discs

Astrophysical discs are often warped, that is, their orbital planes change with radius. This occurs whenever there is a non-axisymmetric force acting on the disc, for example the Lense-Thirring precession induced by a misaligned spinning black hole, or the gravitational pull of a misaligned companion. Such misalignments appear to be generic in astrophysics. The wide range of systems that can harbour warped discs - protostars, X-ray binaries, tidal disruption events, quasars and others - allows for a rich variety in the disc's response. Here we review the basic physics of warped discs and its implications.Comment: To be published in Astrophysical Black Holes by Haardt et al., Lecture Notes in Physics, Springer 2015. 19 pages, 2 figure

On the role of numerical diffusivity in MHD simulations of global accretion disc dynamos

Observations, mainly of outbursts in dwarf novae, imply that the anomalous viscosity in highly ionized accretion discs is magnetic in origin and requires that the plasma β∼1. Until now, most simulations of the magnetic dynamo in accretion discs have used a local approximation (known as the shearing box). While these simulations demonstrate the possibility of a self-sustaining dynamo, the magnetic activity generated in these models saturates at β≫1. This long-standing discrepancy has previously been attributed to the local approximation itself. There have been recent attempts at simulating magnetic activity in global accretion discs with parameters relevant to the dwarf novae. These too find values of β≫1. We speculate that the tension between these models and the observations may be caused by numerical magnetic diffusivity. As a pedagogical example, we present exact time-dependent solutions for the evolution of weak magnetic fields in an incompressible fluid subject to linear shear and magnetic diffusivity. We find that the maximum factor by which the initial magnetic energy can be increased depends on the magnetic Reynolds number as R2/3m. We estimate that current global numerical simulations of dwarf nova discs have numerical magnetic Reynolds numbers around six orders of magnitude less than the physical value found in dwarf nova discs of Rm∼1010. We suggest that, given the current limitations on computing power, expecting to be able to compute realistic dynamo action in observable accretion discs using numerical MHD is, for the time being, a step too far

A trio of month-long flares in the nova-like variable V704 And

We present the discovery of an unusual set of flares in the nova-like variable V704 And. Using data from AAVSO, ASAS-SN, and ZTF for the nova-like variable V704 And, we discovered a trio of brightening events that occurred during the high state. These events elevate the optical brightness of the source from ∼13.5 to ∼12.5 mag. The events last for roughly a month, and exhibit the unusual shape of a slow rise and faster decay. Immediately after the third event, we obtained data from regular monitoring with Swift, although by this time the flares had ceased and the source returned to its pre-flare level of activity in the high state. The Swift observations confirm that during the high state, the source is detectable in the X-rays, and provide simultaneous UV and optical fluxes. As the source is already in the high state prior to the flares, and therefore the disc is expected to already be in the high-viscosity state, we conclude that the driver of the variations must be changes in the mass transfer rate from the companion star and we discuss mechanisms that could lead to such short-timescale mass-transfer variations

Global profiling of the proteomic changes associated with the post-testicular maturation of mouse spermatozoa

Spermatozoa acquire fertilization potential during passage through a highly specialized region of the extratesticular ductal system known as the epididymis. In the absence of de novo gene transcription or protein translation, this functional transformation is extrinsically driven via the exchange of varied macromolecular cargo between spermatozoa and the surrounding luminal plasma. Key among these changes is a substantive remodeling of the sperm proteomic architecture, the scale of which has yet to be fully resolved. Here, we have exploited quantitative mass spectrometry-based proteomics to define the extent of changes associated with the maturation of mouse spermatozoa; reporting the identity of >6,000 proteins, encompassing the selective loss and gain of several hundred proteins. Further, we demonstrate epididymal-driven activation of RHOAmediated signaling pathways is an important component of sperm maturation. These data contribute molecular insights into the complexity of proteomic changes associated with epididymal sperm maturation.David A. Skerrett-Byrne, Amanda L. Anderson, Elizabeth G. Bromfield, Ilana R. Bernstein, Jess E. Mulhall, John E. Schjenken, Matthew D. Dun, Sean J. Humphrey, and Brett Nixo

Immortalized mouse caput epididymal epithelial (mECap18) cell line recapitulates the in-vivo environment

OnlinePublResiding between the testes and the vas deferens, the epididymis is a highly convoluted tubule whose unique luminal microenvironment is crucial for the functional maturation of spermatozoa. This microenvironment is created by the combined secretory and resorptive activity of the lining epididymal epithelium, including the release of extracellular vesicles (epididymosomes), which encapsulate fertility modulating proteins and a myriad of small non-coding RNAs (sncRNAs) that are destined for delivery to recipient sperm cells. To enable investigation of this intercellular communication nexus, we have previously developed an immortalized mouse caput epididymal epithelial cell line (mECap18). Here, we describe the application of label-free mass spectrometry to characterize the mECap18 cell proteome and compare this to the proteome of native mouse caput epididymal epithelial cells. We report the identification of 5,313 mECap18 proteins, as many as 75.8% of which were also identified in caput epithelial cells wherein they mapped to broadly similar protein classification groupings. Furthermore, key pathways associated with protein synthesis (e.g., EIF2 signaling) and cellular protection in the male reproductive tract (e.g., sirtuin signaling) were enriched in both proteomes. This comparison supports the utility of the mECap18 cell line as a tractable in-vitro model for studying caput epididymal epithelial cell function.Jess E. Mulhall, Natalie A. Trigg, Ilana R. Bernstein, Amanda L. Anderson, Heather C. Murray, Petra Sipilä, Tessa Lord, John E. Schjenken, Brett Nixon, David A. Skerrett-Byrn

Clinical perspectives on sampling and processing approaches for the management of infection in diabetic foot ulceration: A qualitative study

Diabetic foot ulcers (DFUs) often become infected and are treated with antimicrobials, with samples collected to inform care. Swab samples are easier than tissue sampling but report fewer organisms. Compared with culture and sensitivity (C&S) methods, molecular microbiology identifies more organisms. Clinician perspectives on sampling and processing are unknown. We explored clinician perspectives on DFU sampling—tissue samples/wound swabs—and on processing techniques, culture and sensitivity or molecular techniques. The latter provides information on organisms which have not survived transport to the laboratory for culture. We solicited feedback on molecular microbiology reports. Qualitative study using semi-structured interview, with analysis using a Framework approach. CODIFI2 clinicians from UK DFU clinics. Seven consultants agreed to take part. They reported, overall, a preference for tissue samples over swabbing. Clinicians were not confident replacing C&S with molecular microbiology as the approach to reporting was unfamiliar. The study was small and did not recruit any podiatrists or nurses, who may have discipline-specific attitudes or perspectives on DFU care. Both sampling approaches appear to be used by clinicians. Molecular microbiology reports would not be, at present, suitable for replacement of traditional culture and sensitivity

Asynchronous optical sampling of on-chip terahertz devices for real-time sensing and imaging applications

We demonstrate that asynchronous optical sampling (ASOPS) can be used to measure the propagation of terahertz (THz) bandwidth pulses in a coplanar waveguide device with integrated photoconductive switches used for signal excitation and detection. We assess the performance of the ASOPS technique as a function of measurement duration, showing the ability to acquire full THz time-domain traces at rates up to 100 Hz. We observe a peak dynamic range of 40 dB for the shortest measurement duration of 10 ms, increasing to 88 dB with a measurement time of 500 s. Our work opens a route to real-time video-rate imaging via modalities using scanned THz waveguides, as well as real-time THz sensing of small volume analytes; we benchmark our on-chip ASOPS measurements against previously published simulations of scanning THz sensor devices, demonstrating sufficient dynamic range to underpin future video-rate THz spectroscopy measurements with these devices

Supermassive Black Hole Binaries: The Search Continues

Gravitationally bound supermassive black hole binaries (SBHBs) are thought to be a natural product of galactic mergers and growth of the large scale structure in the universe. They however remain observationally elusive, thus raising a question about characteristic observational signatures associated with these systems. In this conference proceeding I discuss current theoretical understanding and latest advances and prospects in observational searches for SBHBs.Comment: 17 pages, 4 figures. To appear in the Proceedings of 2014 Sant Cugat Forum on Astrophysics. Astrophysics and Space Science Proceedings, ed. C.Sopuerta (Berlin: Springer-Verlag

Transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamide

Background: The seminal vesicles synthesise bioactive factors that support gamete function, modulate the female reproductive tract to promote implantation, and influence developmental programming of offspring phenotype. Despite the significance of the seminal vesicles in reproduction, their biology remains poorly defined. Here, to advance understanding of seminal vesicle biology, we analyse the mouse seminal vesicle transcriptome under normal physiological conditions and in response to acute exposure to the reproductive toxicant acrylamide. Mice were administered acrylamide (25 mg/kg bw/day) or vehicle control daily for five consecutive days prior to collecting seminal vesicle tissue 72 h following the final injection. Results: A total of 15,304 genes were identified in the seminal vesicles with those encoding secreted proteins amongst the most abundant. In addition to reproductive hormone pathways, functional annotation of the seminal vesicle transcriptome identified cell proliferation, protein synthesis, and cellular death and survival pathways as prominent biological processes. Administration of acrylamide elicited 70 differentially regulated (fold-change ≥1.5 or ≤ 0.67) genes, several of which were orthogonally validated using quantitative PCR. Pathways that initiate gene and protein synthesis to promote cellular survival were prominent amongst the dysregulated pathways. Inflammation was also a key transcriptomic response to acrylamide, with the cytokine, Colony stimulating factor 2 (Csf2) identified as a top-ranked upstream driver and inflammatory mediator associated with recovery of homeostasis. Early growth response (Egr1), C-C motif chemokine ligand 8 (Ccl8), and Collagen, type V, alpha 1 (Col5a1) were also identified amongst the dysregulated genes. Additionally, acrylamide treatment led to subtle changes in the expression of genes that encode proteins secreted by the seminal vesicle, including the complement regulator, Complement factor b (Cfb). Conclusions: These data add to emerging evidence demonstrating that the seminal vesicles, like other male reproductive tract tissues, are sensitive to environmental insults, and respond in a manner with potential to exert impact on fetal development and later offspring health.David A. Skerrett-Byrne, Brett Nixon, Elizabeth G. Bromfield, James Breen, Natalie A. Trigg, Simone J. Stanger, Ilana R. Bernstein, Amanda L. Anderson, Tessa Lord, R. John Aitken, Shaun D. Roman, Sarah A. Robertson, and John E. Schjenke