Comparing Data Quality and Cost from Three Modes of On-Board Transit Passenger Surveys

This report presents the findings from a research project investigating the relative data quality and administration costs for three different modes of surveying bus passengers that produce results generalizable to the full passenger population. The three modes, all of which used survey methods distributed or administered onboard the transit vehicle, were: self-complete paper surveys, self-complete online surveys, and interviewer-assisted tablet-based surveys. Results from this study indicate several implications for practitioners choosing a survey mode. First, and most importantly, the analysis reinforces the point that there is no single, best survey mode. The choice of mode must depend on an agency’s priorities for what questions most need to be answered, what population groups are most important to represent, and exactly how the agency chooses to define concepts like a “complete” survey or a “usable” address. Findings suggest several general recommendations for current survey practice: (1) online surveys administered via an invitation distributed on the transit vehicle are not a good option; (2) old-fashioned, low-tech paper survey may still be the best option for many bus passenger surveys; (3) changes in survey results that accompany changes in survey methods should be interpreted with caution; and (4) using a new survey method, especially one relying on more complex technologies, may create unexpected glitches

Age and Growth of Larval and Juvenile Atlantic Croaker, Micropogonias Undulatus, from the Middle Atlantic Bight and Estuarine Waters of Virginia

Sagittal otoliths were used to determine age and growth of 605 larval and juvenile Atlantic croaker, Micropogonias undulatus, collected in the Middle Atlantic Bight and estuarine waters of Virginia. This study is the first to use age-based analysis for young Atlantic croaker collected in this region. A Laird-Gompertz model (r2=0.95) was used to describe the growth of Atlantic croaker up to 65 mm standard length (SL) and 142 days (t): SL((t)) = 2.657 exp (4.656 [1-exp (-0.0081t)]); where SL((t)) = standard length at day t. Spatial and temporal patterns in the size and age of Atlantic croaker showed a pattern of inshore immigration from offshore spawning grounds, and faster early-season growth compared with late-season growth. Back-calculated hatching dates of Atlantic croaker collected in Virginia estuaries indicated a protracted spawning period over 8 months, from early July 1987 to early February 1988, with at least 82% of spawning occurring from August to October. Regression analysis indicated that early-spawned larvae (July through August) grew more than 39% faster than late-spawned larvae (September through February). Lapillar and sagittal otoliths were compared with light microscopy; ages were under-estimated with lapillar otoliths, which were particularly inadequate in determining the age of older juveniles. The relation between SL and sagittal otolith maximum diameter was best described by a fourth order polynomial (r2=0.99) and faster-growing Atlantic croaker had larger otoliths (12%) than the same size slower-growing fish

LOCATION OF A MIXALCO PRODUCTION FACILITY WITH RESPECT TO ECONOMIC VIABILITY

Monte-Carlo simulation modeling is used to perform a feasibility study of alternative locations for a MixAlco production facility. Net present value distributions will be ranked within feasible risk aversion boundaries. If MixAlco is a profitable investment, it would have a major impact on the fuel oxygenate and gasoline markets.Resource /Energy Economics and Policy,

Effect of water-soluble polymers, polyethylene glycol and poly(vinylpyrrolidone),on the gelation of aqueous micellar solutions of Pluronic copolymer F127

The micellization of F127 (E98P67E98) in dilute aqueous solutions of polyethylene glycol (PEG6000 and PEG35000) and poly(vinylpyrrolidone) (PVP K30 and PVP K90) is studied. The average hydrodynamic radius (rh,app) obtained from the dynamic light scattering technique increased with increase in PEG concentration but decreased on addition of PVP, results which are consistent with interaction of the micelles with PEG and the formation of micelles clusters, but no such interaction occurs with PVP. Tube inversion was used to determine the onset of gelation. The critical concentration of F127 for gelation increased on addition of PEG and of PVP K30 but decreased on addition of PVP K90. Small-angle X-ray scattering (SAXS) was used to show that the 30 wt% F127 gel structure (fcc) was independent of polymer type and concentration, as was the d-spacing and so the micelle hard-sphere radius. The maximum elastic modulus (G0 max) of 30 wt% F127 decreased from its value for water alone as PEG was added, but was little changed by adding PVP. These results are consistent with the packed-micelles in the 30 wt% F127 gel being effectively isolated from the polymer solution on the microscale while, especially for the PEG, being mixed on the macroscale

A one hundred and seventeen year coastal water temperature record from Woods Hole,

ABSTRACT: We have compiled what we believe is the longest coherent coastal sea surface temperature record in North America. Near-surface water temperature measurements have been made almost daily at Great Harbor, Woods Hole, Massachusetts, since 1886 with remarkably few gaps. The record shows that there was no significant trend in water temperature at this site for the first 60 yr of observation. There was some cooling during the 1960s that was followed by a significant warming from 1970-2002 at a rate of 0.04؇C yr Ϫ1 . During the 1990s annual mean temperatures averaged approximately 1.2؇C warmer than they had been on average between 1890 and 1970; winter (December, January, and February) temperatures were 1.7؇C warmer and summer (June, July, and August) temperatures were 1.0؇C warmer. There has not been a statistically significant decrease in the annual number of winter days below 1؇C or an increase in the annual number of winter days above 5؇C. The number of summer days each year with water temperature above 21؇C has not increased significantly. The dates of first observations of 10؇C and 20؇C water in the spring have not changed sufficiently to be statistically significant. There is a weak positive correlation between annual and winter water temperature and the annual and winter North Atlantic Oscillation index, respectively, during the period of record

Mitotic stress is an integral part of the oncogene-induced senescence program that promotes multinucleation and cell cycle arrest

Oncogene-induced senescence (OIS) is a tumor suppression mechanism that blocks cell proliferation in response to oncogenic signaling. OIS is frequently accompanied by multinucleation; however, the origin of this is unknown. Here, we show that multinucleate OIS cells originate mostly from failed mitosis. Prior to senescence, mutant H-RasV12 activation in primary human fibroblasts compromised mitosis, concordant with abnormal expression of mitotic genes functionally linked to the observed mitotic spindle and chromatin defects. Simultaneously, H-RasV12 activation enhanced survival of cells with damaged mitoses, culminating in extended mitotic arrest and aberrant exit from mitosis via mitotic slippage. ERK-dependent transcriptional upregulation of Mcl1 was, at least in part, responsible for enhanced survival and slippage of cells with mitotic defects. Importantly, mitotic slippage and oncogene signaling cooperatively induced senescence and key senescence effectors p21 and p16. In summary, activated Ras coordinately triggers mitotic disruption and enhanced cell survival to promote formation of multinucleate senescent cells

Mitotic spindle association of TACC3 requires Aurora-A-dependent stabilization of a cryptic α-helix.

Aurora-A regulates the recruitment of TACC3 to the mitotic spindle through a phospho-dependent interaction with clathrin heavy chain (CHC). Here, we describe the structural basis of these interactions, mediated by three motifs in a disordered region of TACC3. A hydrophobic docking motif binds to a previously uncharacterized pocket on Aurora-A that is blocked in most kinases. Abrogation of the docking motif causes a delay in late mitosis, consistent with the cellular distribution of Aurora-A complexes. Phosphorylation of Ser558 engages a conformational switch in a second motif from a disordered state, needed to bind the kinase active site, into a helical conformation. The helix extends into a third, adjacent motif that is recognized by a helical-repeat region of CHC, not a recognized phospho-reader domain. This potentially widespread mechanism of phospho-recognition provides greater flexibility to tune the molecular details of the interaction than canonical recognition motifs that are dominated by phosphate binding

Fault interactions and reactivation within a normal-fault network at Milne Point, Alaska

A normal-fault network from Milne Point, Alaska, is investigated focusing on characterizing geometry, displacement, strain, and different fault interactions. The network, constrained from three-dimensional seismic reflection data, comprises two generations of faults: Cenozoic north-northeast–trending faults and Jurassic west-northwest–trending faults, which highly compartmentalize Upper Triassic to Lower Cretaceous reservoirs. The west-northwest–trending faults are influenced by a similarly oriented underlying structural grain. This influence is characterized by increases in throw on several faults, strain localization, reorientation of faults and an increase in linkage maturity.Reconstructing fault plane geometries and mapping spatial variations in throw identified key characteristic features in their interactions and reactivation of pre-existing structures. Faults are divided into isolated, abutting, and splaying faults. Isolated faults exhibit a range of displacement profiles depending on the degree of restriction at fault tips. Fault splays accommodate step-like decreases in throw along larger main faults with a throw maximum at the intersection with the main fault. Throw profiles of abutting faults are divided into two groups: early stage abutting faults with throw minima at both the isolated and abutting tips, and developed abutting faults with throw maxima near the abutting tip.Developed abutting faults accumulate throw after initial abutment, locally reactivating and transferring throw onto the pre-existing fault. Two abutting faults can link kinematically by reactivating a segment of the pre-existing fault forming a trailing fault. The motion sense of the trailing fault can be synthetic or antithetic to the reactivated pre-existing fault, producing increases or decreases in the throw of the pre-existing fault, respectivel

Exoplanet Diversity in the Era of Space-based Direct Imaging Missions

This whitepaper discusses the diversity of exoplanets that could be detected by future observations, so that comparative exoplanetology can be performed in the upcoming era of large space-based flagship missions. The primary focus will be on characterizing Earth-like worlds around Sun-like stars. However, we will also be able to characterize companion planets in the system simultaneously. This will not only provide a contextual picture with regards to our Solar system, but also presents a unique opportunity to observe size dependent planetary atmospheres at different orbital distances. We propose a preliminary scheme based on chemical behavior of gases and condensates in a planet's atmosphere that classifies them with respect to planetary radius and incident stellar flux.Comment: A white paper submitted to the National Academy of Sciences Exoplanet Science Strateg

Macroautophagy—a novel β-amyloid peptide-generating pathway activated in Alzheimer's disease

Macroautophagy, which is a lysosomal pathway for the turnover of organelles and long-lived proteins, is a key determinant of cell survival and longevity. In this study, we show that neuronal macroautophagy is induced early in Alzheimer's disease (AD) and before β-amyloid (Aβ) deposits extracellularly in the presenilin (PS) 1/Aβ precursor protein (APP) mouse model of β-amyloidosis. Subsequently, autophagosomes and late autophagic vacuoles (AVs) accumulate markedly in dystrophic dendrites, implying an impaired maturation of AVs to lysosomes. Immunolabeling identifies AVs in the brain as a major reservoir of intracellular Aβ. Purified AVs contain APP and β-cleaved APP and are highly enriched in PS1, nicastrin, and PS-dependent γ-secretase activity. Inducing or inhibiting macroautophagy in neuronal and nonneuronal cells by modulating mammalian target of rapamycin kinase elicits parallel changes in AV proliferation and Aβ production. Our results, therefore, link β-amyloidogenic and cell survival pathways through macroautophagy, which is activated and is abnormal in AD