The application of electron microscopy to the study of plant viruses in unpurified plant extracts

Rods of variable lengths occurred in sap from plants infected with tobacco mosaic, cucumber mosaic, potato X, potato Y, henbane mosaic, tobacco etch, and cabbage blackring viruses; the first two were about 15 mμ. wide and appeared rigid, the others were about 10 mμ. wide and apparently flexible. Sap from plants infected with tomato bushy stunt, tobacco ringspot and two tobacco necrosiss viruses contained spherical particles about 26 mμ. in diameter; two particles, one about 18 mμ. and the other about 37 mμ. in diameter occurred in sap from plants infected with a third tobacco necrosis virus. No specific particles were identified in sap from plants infected with tomato spotted wilt, potato leaf roll, cauliflower mosaic, tomato aspermy, sugar beet mosaic and sugar beet yellows viruses. Serologically related strains of any one virus were morphologically indistinguishable, but this has little diagnostic value because so also were some unrelated viruses

Plant cover, soil temperature, freeze, water stress, and evapotranspiration conditions

There are no author-identified significant results in this report

The mechanical transmission and some properties of potato paracrinkle virus

With the aid of abrasives, paracrinkle virus, hitherto transmitted only by grafting, was transmitted to Arran Victory potatoes by inoculation with sap from infected plants, either symptomless King Edward or diseased Arran Victory. The proportion of plants that became infected was increased when they were kept in darkness for some days before inoculation. Tomato plants were more readily infected than Arran Victory potatoes, no abrasive being needed. Infected tomatoes were symptomless, but electron microscopy showed their sap to contain rod-shaped particles of variable lengths and about 10 mμ. wide. Such particles have not been found in uninfected tomatoes and they are presumed to be the virus; they were destroyed by heating at 60°. The nature of similar particles in King Edward and Arran Victory potatoes with paracrinkle is uncertain, because rod-shaped particles were also found in uninfected Arran Victory. Rod-shaped particles also occurred in uninfected plants of all other potato varieties examined and in newly raised potato seedlings; they were not transmitted, either by inoculation or by grafting, to tomato or other hosts tested, and they withstood heating to 98°. These results with paracrinkle parallel those with certain other plant viruses. They invalidate theories that postulate its origin as a consequence of grafting and render unnecessary the concept that it is intrinsic to King Edward potatoes

Plant cover, soil temperature, freeze, water stress, and evapotranspiration conditions

There are no author-identified significant results in this report

AgRISTARS: Early warning and crop condition assessment. Plant cover, soil temperature, freeze, water stress, and evapotranspiration conditions

Emissive (10.5 to 12.5 microns) and reflective (0.55 to 1.1 microns) data for ten day scenes and infrared data for six night scenes of southern Texas were analyzed for plant cover, soil temperature, freeze, water stress, and evapotranspiration. Heat capacity mapping mission radiometric temperatures were: within 2 C of dewpoint temperatures, significantly correlated with variables important in evapotranspiration, and related to freeze severity and planting depth soil temperatures

Plant cover, soil temperature, freeze, water stress, and evapotranspiration conditions

There are no author-identified significant results in this report

Plant cover, soil temperature, freeze, water stress, and evapotranspiration conditions

The author has identified the following significant results. HCMM day/night coverage 12 hours apart cannot be obtained at 26 deg N latitude; nor have any pairs 36 hours apart been obtained. A day-IR scene and a night scene for two different dates were analyzed. A profile across the test site for the same latitude shows that the two profiles are near mirror images of each other over land surfaces and that the temperature of two large water bodies, Falcon Reservoir and the Gulf of Mexico, are nearly identical on two dates. During the time interval between overpasses, the vegetative cover remained static due to winter dormancy. The data suggest that day/night temperature differences measured weeks apart may yield meaningful information about the contrast between daytime maximum and nighttime minimum temperatures for a given site

Plant cover, soil temperature, freeze, water stress, and evapotranspiration conditions

The author has identified the following significant results. Procedures to edit cloud-contaminated pixels from those pixels representing Earth surface features were investigated. Because clouds are more reflective than Earth features and are colder than Earth surface features most of the year at 26 N latitude, either a raw digital count ratio or a ratio of reflectance percentage for the VIS band to the temperature works well. For this procedure, the two bands of data need to be registered to the ground scene

Plant cover, soil temperature, freeze, water stress, and evapotranspiration

There are no author-identified significant results in this report

Plant cover, soil temperature, freeze, water stress, and evapotranspiration conditions

Emissive and reflective data for 10 days, and IR data for 6 nights in south Texas scenes were analyzed after procedures were developed for removing cloud-affected data. HCMM radiometric temperatures were: within 2 C of dewpoint temperatures on nights when air temperature approached dewpoint temperatures; significantly correlated with variables important in evapotranspiration; and, related to freeze severity and planting depth soil temperatures. Vegetation greenness indexes calculated from visible and reflective IR bands of NOAA-6 to -9 meteorological satellites will be useful in the AgRISTARS program for seasonal crop development, crop condition, and drought applications