Kajian Populasi Kepiting Kenari Di Pulau Batudaka Kepulauan Togean, Sulawesi Tengah Dan Rekomendasi Manajemen Populasi

This study aimed to quantify the population of Birgus latro in the Batudaka di Togean islands, Central Sulawesi. The research on robber crab was conducted in Batudaka Island, Togean, Tomini Bay, Central Sulawesi. In the study site, 21 plots measuring of 50x50 m2 were created bounded by raffia. Feed in the form of shredded coconut is placed in each plot in the afternoon. At night was performed observations and catchs. In the "base camp" every crab crab carapace caught measured in carapace length and weight. During the study, 277 crabs were caught, consisted of 173 males (62.45%) and 104 (37.55%) females. Based on the formula calculation of Schiller (1992) population figures obtained 821 803 ± 195 030 crabs in Batudaka Island. By regression analysis between carapace length with weight, it was found that the growth of B. latro is negative allometric, i.e., weight gain is faster than the increase length of carapace. The weight gain of female is slightly higher than that of the male. Whether male crab population or female equally composed of 9 age groups. This study showed that 66.7% of male crab and 29.1% of female crab has entered the market size

Building droplet-based microfluidic systems for biological analysis

Abstract In the present paper, we review and discuss current developments and challenges in the field of dropletbased microfluidics. This discussion includes an assessment of the basic fluid dynamics of segmented flows, material requirements, fundamental unit operations and how integration of functional components can be applied to specific biological problems

Fluorescence detection methods for microfluidic droplet platforms

The development of microfluidic platforms for performing chemistry and biology has in large part been driven by a range of potential benefits that accompany system miniaturisation. Advantages include the ability to efficiently process nano- to femoto- liter volumes of sample, facile integration of functional components, an intrinsic predisposition towards large-scale multiplexing, enhanced analytical throughput, improved control and reduced instrumental footprints.

A fully unsupervised compartment-on-demand platform for precise nanoliter assays of time-dependent steady-state enzyme kinetics and inhibition.

The ability to miniaturize biochemical assays in water-in-oil emulsion droplets allows a massive scale-down of reaction volumes, so that high-throughput experimentation can be performed more economically and more efficiently. Generating such droplets in compartment-on-demand (COD) platforms is the basis for rapid, automated screening of chemical and biological libraries with minimal volume consumption. Herein, we describe the implementation of such a COD platform to perform high precision nanoliter assays. The coupling of a COD platform to a droplet absorbance detection set-up results in a fully automated analytical system. Michaelis-Menten parameters of 4-nitrophenyl glucopyranoside hydrolysis by sweet almond β-glucosidase can be generated based on 24 time-courses taken at different substrate concentrations with a total volume consumption of only 1.4 μL. Importantly, kinetic parameters can be derived in a fully unsupervised manner within 20 min: droplet production (5 min), initial reading of the droplet sequence (5 min), and droplet fusion to initiate the reaction and read-out over time (10 min). Similarly, the inhibition of the enzymatic reaction by conduritol B epoxide and 1-deoxynojirimycin was measured, and Ki values were determined. In both cases, the kinetic parameters obtained in droplets were identical within error to values obtained in titer plates, despite a >10(4)-fold volume reduction, from micro- to nanoliters

Droplet microfluidic-based in situ analyzer for monitoring free nitrate in soil

Monitoring nutrients in the soil can provide valuable information for understanding their spatiotemporal variability and informing precise soil management. Here, we describe an autonomous in situ analyzer for the real-time monitoring of nitrate in soil. The analyzer can sample soil nitrate using either microdialysis or ultrafiltration probes placed within the soil and quantify soil nitrate using droplet microfluidics and colorimetric measurement. Compared with traditional manual sampling and lab analysis, the analyzer features low reagent consumption (96 μL per measurement), low maintenance requirement (monthly), and high measurement frequency (2 or 4 measurements per day),providing nondrifting lab-quality data with errors of less than 10% using a microdialysis probe and 2−3% for ultrafiltration. The analyzer was deployed at both the campus garden and forest for different periods of time, being able to capture changes in free nitrate levels in response to manual perturbation by the addition of nitrate standard solutions and natural perturbation by rainfall events

Combinatorial microfluidic droplet engineering for biomimetic material synthesis

Although droplet-based systems are used in a wide range of technologies, opportunities for systematically customizing their interface chemistries remain relatively unexplored. This article describes a new microfluidic strategy for rapidly tailoring emulsion droplet compositions and properties. The approach utilizes a simple platform for screening arrays of droplet-based microfluidic devices and couples this with combinatorial selection of the droplet compositions. Through the application of genetic algorithms over multiple screening rounds, droplets with target properties can be rapidly generated. The potential of this method is demonstrated by creating droplets with enhanced stability, where this is achieved by selecting carrier fluid chemistries that promote titanium dioxide formation at the droplet interfaces. The interface is a mixture of amorphous and crystalline phases, and the resulting composite droplets are biocompatible, supporting in vitro protein expression in their interiors. This general strategy will find widespread application in advancing emulsion properties for use in chemistry, biology, materials and medicine

Efficient spatial-temporal chaotic mixing in microchannels

A chaotic micro mixer with multiple side channels is designed and investigated, in which fluid can be stirred by pumps through the side channels. By stretching and folding fluid in the main and side channels, chaotic mixing can be achieved. A simple mathematic model is derived to understand the movement of particles in the microchannel. Spatial trajectories of fluid particles are projected to Poincaré sections by mapping. The route from the quasi-period to chaos is revealed to be destruction of KAM curves and shrinkage of the quasi-periodic areas. Lyapunov exponents (LE) are used as the mixing index and the criteria to evaluate the chaotic behavior of the system. We found that LE is closely related to the amplitude and frequency of stirring and can be used to optimize our design and operation. From the relationship of LE and striation thickness, the minimal mixing length required can be estimated, which is much shorter than that needed in passive mixer design.<br/

Sensitive absorbance measurement in droplet microfluidics via multipass flow cells

Absorbance measurement is a useful analytical tool that can be used along with colorimetric assays to measure a wide range of analytes. However, since sensitivity is directly proportional to path length, sensitive measurements in microfluidic channels are inherently challenging. This is especially true for droplet microfluidics where the lensing at the droplet/carrier interface further constrains path length. In this study, we developed multipass flow cells assembled with squared PTFE tube with parallel mirrors on both sides, laser diode and detector. The devices featured affordable low power components, and was made using simple fabrication techniques making it accessible to a wide range of researchers. In testing it allowed multiple reflection of light in the detection chamber, which significantly increased the optical path length by 8 times. The flow cell was used to quantify the phosphate levels in water samples from a tidal chalk river which could not be measured with a simple single-pass flow cell