Injury Rates in Age-Only Versus Age-and-Weight Playing Standard Conditions in American Youth Football

Background: American youth football leagues are typically structured using either age-only (AO) or age-and-weight (AW) playing standard conditions. These playing standard conditions group players by age in the former condition and by a combination of age and weight in the latter condition. However, no study has systematically compared injury risk between these 2 playing standards. Purpose: To compare injury rates between youth tackle football players in the AO and AW playing standard conditions. Study Design: Cohort study; Level of evidence, 2. Methods: Athletic trainers evaluated and recorded injuries at each practice and game during the 2012 and 2013 football seasons. Players (age, 5-14 years) were drawn from 13 recreational leagues across 6 states. The sample included 4092 athlete-seasons (AW, 2065; AO, 2027) from 210 teams (AW, 106; O, 104). Injury rate ratios (RRs) with 95% CIs were used to compare the playing standard conditions. Multivariate Poisson regression was used to estimate RRs adjusted for residual effects of age and clustering by team and league. There were 4 endpoints of interest: (1) any injury, (2) non–time loss (NTL) injuries only, (3) time loss (TL) injuries only, and (4) concussions only. Results: Over 2 seasons, the cohort accumulated 1475 injuries and 142,536 athlete-exposures (AEs). The most common injuries were contusions (34.4%), ligament sprains (16.3%), concussions (9.6%), and muscle strains (7.8%). The overall injury rate for both playing standard conditions combined was 10.3 per 1000 AEs (95% CI, 9.8-10.9). The TL injury, NTL injury, and concussion rates in both playing standard conditions combined were 3.1, 7.2, and 1.0 per 1000 AEs, respectively. In multivariate Poisson regression models controlling for age, team, and league, no differences were found between playing standard conditions in the overall injury rate (RRoverall, 1.1; 95% CI, 0.4-2.6). Rates for the other 3 endpoints were also similar (RRNTL, 1.1 [95% CI, 0.4-3.0]; RRTL, 0.9 [95% CI, 0.4-1.9]; RRconcussion, 0.6 [95% CI, 0.3-1.4]). Conclusion: For the injury endpoints examined in this study, the injury rates were similar in the AO and AW playing standards. Future research should examine other policies, rules, and behavioral factors that may affect injury risk within youth football

A diarylamine derived from anthranilic acid inhibits ZIKV replication

Zika virus (ZIKV) is a mosquito-transmitted Flavivirus, originally identified in Uganda in 1947 and recently associated with a large outbreak in South America. Despite extensive efforts there are currently no approved antiviral compounds for treatment of ZIKV infection. Here we describe the antiviral activity of diarylamines derived from anthranilic acid (FAMs) against ZIKV. A synthetic FAM (E3) demonstrated anti-ZIKV potential by reducing viral replication up to 86%. We analyzed the possible mechanisms of action of FAM E3 by evaluating the intercalation of this compound into the viral dsRNA and its interaction with the RNA polymerase of bacteriophage SP6. However, FAM E3 did not act by these mechanisms. In silico results predicted that FAM E3 might bind to the ZIKV NS3 helicase suggesting that this protein could be one possible target of this compound. To test this, the thermal stability and the ATPase activity of the ZIKV NS3 helicase domain (NS3Hel) were investigated in vitro and we demonstrated that FAM E3 could indeed bind to and stabilize NS3Hel

The Interrelationship and Diagnostic Utility of Memory and Reaction Time in Concussed Students: 971 Board #232 May 30 3

More than 40 million American youth participate in interscholastic, community-based, and collegiate sports. A risk of participation is traumatic brain injury (TBI). In up to 40% of TBI cases, athletes experience persistent functional and cognitive deficits. It is important to understand the variables that lead to these deficits to improve diagnosis and prognostic management. PURPOSE: To evaluate memory and reaction time as markers of TBI severity among patients experiencing prolonged recovery. METHODS: We retrospectively analyzed student-athletes admitted to a Midwestern outpatient clinic for neuropsychological evaluation; 78 patients had relatively comprehensive profiles and were included in the analysis. We conducted a health history, a 22-item post-concussion symptom inventory, and the ImPACT computerized test, which evaluated memory and reaction time. Pearson’s and point-biserial correlation coefficients tested the direction and strength of association between memory, reaction time, and markers of injury severity. Logistic, negative binomial, and linear regressions tested memory and reaction time as predictors of whether symptoms were reported, the number of reported symptoms, and the severity of symptoms. RESULTS: Patients were 16.0 ± 2.6 years of age, 56.3% were male, and they had experienced 1.2 ± 1.5 previous concussions. Reaction time was 0.64 ± 0.13 seconds; visual motor speed score was 44.7 ± 34.6; visual memory score was 92.0 ± 69.3; verbal memory score was 98.0 ± 80.9; cognitive efficiency score was 0.34 ± 0.12. Reaction time was a significant predictor (p\u3c0.05) of balance problems, dizziness, mental fogginess, and sensitivity to light and noise; it was a trending predictor (p=0.061) of the summed severity of symptoms. Verbal memory was a significant predictor (p\u3c0.05) of balance problems, sleeping problems, and fatigue. Visual memory, visual motor speed, and cognitive efficiency index were poor predictors of injury severity. CONCLUSIONS: Reaction time and memory are common components of testing batteries for concussed athletes. In our sample, reaction time and verbal memory emerged as useful predictors of severity among patients suffering long-term symptoms of TBI. It may be of value for coaches and athletic trainers to establish baseline values at the onset of a competitive season

7-(4-Methoxyphenyl)-4,9-dimethyl-N-(4-methylphenyl)-5,12-diazatetraphen-6-amine

In the title compound, C32H27N3O, the fused tetracycilc ring system is essentially planar [r.m.s. deviation = 0.07 (7) Å]. An intramolecular N—H...π(arene) interaction and a weak intramolecular C—H...N hydrogen bond may influence the molecular conformation. In the crystal, weak intermolecular C—H...N hydrogen bonds link the molecules into centrosymmetric dimers, forming R22(14) motifs. In addition, weak π–π stacking interactions with centroid–centroid distances in the range 3.578 (1)–3.739 (1) Å provide further stabilization

Loss of circadian gene Timeless induces EMT and tumor progression in colorectal cancer via Zeb1-dependent mechanism

The circadian gene Timeless (TIM) provides a molecular bridge between circadian and cell cycle/DNA replication regulatory systems and has been recently involved in human cancer development and progression. However, its functional role in colorectal cancer (CRC), the third leading cause of cancer-related deaths worldwide, has not been fully clarified yet. Here, the analysis of two independent CRC patient cohorts (total 1159 samples) reveals that loss of TIM expression is an unfavorable prognostic factor significantly correlated with advanced tumor stage, metastatic spreading, and microsatellite stability status. Genome-wide expression profiling, in vitro and in vivo experiments, revealed that TIM knockdown induces the activation of the epithelial-to-mesenchymal transition (EMT) program. Accordingly, the analysis of a large set of human samples showed that TIM expression inversely correlated with a previously established gene signature of canonical EMT markers (EMT score), and its ectopic silencing promotes migration, invasion, and acquisition of stem-like phenotype in CRC cells. Mechanistically, we found that loss of TIM expression unleashes ZEB1 expression that in turn drives the EMT program and enhances the aggressive behavior of CRC cells. Besides, the deranged TIM-ZEB1 axis sets off the accumulation of DNA damage and delays DNA damage recovery. Furthermore, we show that the aggressive and genetically unstable ‘CMS4 colorectal cancer molecular subtype’ is characterized by a lower expression of TIM and that patients with the combination of low-TIM/high-ZEB1 expression have a poorer outcome. In conclusion, our results as a whole suggest the engagement of an unedited TIM-ZEB1 axis in key pathological processes driving malignant phenotype acquisition in colorectal carcinogenesis. Thus, TIM-ZEB1 expression profiling could provide a robust prognostic biomarker in CRC patients, supporting targeted therapeutic strategies with better treatment selection and patients’ outcomes