757 research outputs found
Forest harvesting impacts on microclimate conditions and sediment transport activities in a humid periglacial environment
Sediment transport activities in periglacial environments
are controlled by microclimate conditions (i.e., air and ground temperatures,
throughfall), which are highly affected by vegetation cover. Thus, there is
the possibility that forest harvesting, the most dramatic change to
vegetation cover in mountain areas, may severely impact sediment transport
activities in periglacial areas (i.e., soil creep, dry ravel). In this study,
we investigated changes in sediment transport activities following forest
harvesting in steep artificial forests located in a humid periglacial area of
the southern Japanese Alps. In the southern Japanese Alps, rainfall is
abundant in summer and autumn, and winter air temperatures frequently rise
above and fall below 0∘. Our monitoring by time lapse cameras
revealed that gravitational transport processes (e.g., frost creep and dry
ravel) dominate during the freeze–thaw season, while rainfall-induced
processes (surface erosion and soil creep) occur during heavy rainfall
seasons. Canopy removal by forest harvesting increased the winter diurnal
ground surface temperature range from 2.7 to 15.9 ∘C. Forest
harvesting also increased the diurnal range of net radiation and ground
temperature, and decreased the duration of snow cover. Such changes in the
microclimate conditions altered the type of winter soil creep from frost
creep to diurnal needle-ice creep. Winter creep velocity of ground surface
sediment in the harvested site (> 2 mm day−1 on the days
with frost heave) was significantly higher than that in the non-harvested
site (generally < 1 mm day−1). Meanwhile, sediment flux on
the hillslopes, as observed by sediment traps, decreased in the harvested
site. Branches of harvested trees left on the hillslopes captured sediment
moving downslope. In addition, the growth of understories after harvesting
possibly reduced surface erosion. Consequently, removal of the forest canopy
by forest harvesting directly impacts the microclimate conditions (i.e.,
diurnal range of ground temperature and net radiation, duration of snow
cover) and increases frequency and velocity of periglacial soil creep, while
sediment flux on hillslopes is decreased by branches left on the hillslopes
and recovery of understories. The impact of forest harvesting on sediment
transport activity is seasonally variable in humid periglacial areas, because
microclimate conditions relevant to both freeze–thaw processes and
precipitation-induced processes control sediment transport.</p
Cytokinin biosynthesis ISOPENTENYLTRANSFERASE genes are differentially expressed during phyllomorph development in the acaulescent Streptocarpus rexii (Gesneriaceae)
Abstract The enzyme ISOPENTENYLTRANSFERASE (IPT) is responsible for the rate limiting step of cytokinin biosynthesis, an important plant hormone with key roles in meristem maintenance and organ development. In this study, we isolated IPT genes from the acaulescent Streptocarpus rexii, a plant that shows an unorthodox development starting with post-germination anisocotyly, in which cytokinins play an integral role. Three adenosine phosphate-IPTs and two tRNA-IPTs were isolated from S. rexii. Their expression levels and patterns in different tissues were compared by means of realtime-PCR and mRNA in-situ hybridization. We found that each SrIPT had a distinctive expression pattern. Interestingly, in vegetative tissues as well as in meristems only the adenosine phosphate-IPT SrIPT5 and the tRNA-IPT SrIPT9 were found. In addition, they were differentially affected by external hormone application, suggesting their different regulation and expression during meristem formation and maintenance and lamina growth. Our results indicate that SrIPTs are involved in shaping the architecture of S. rexii, working differentially and redundantly, and show that differentially expressed IPT genes regulate plant form
Cardiovascular magnetic resonance tagging imaging correlates with myocardial dysfunction and T2 mapping in idiopathic dilated cardiomyopathy
To evaluate the details of myocardial dysfunction in dilated cardiomyopathy (DCM) patients using tagging images and the correlation of tagging imaging with tissue characteristics. C
Gibberellin as a suppressor of lateral dominance snd inducer of apical growth in the unifoliate Streptocarpus wendlandii (Gesneriaceae)
We report on the effects of exogenously applied hormones on the lateral and apical dominance that governs morphogenesis in the unifoliate Streptocarpus wendlandii. In this phenotype, lateral dominance is extreme as the plants only retain a macrocotyledon that develops into a leaf-like phyllomorph by means of a basal meristem and do not show apical growth. Gibberellin applications suppressed the basal meristem activity of the macrocotyledon resulting in an isocotylous seedling with two microcotyledons and caused the formation of a primary phyllomorph, which suggests that the groove meristem, a shoot apical meristem equivalent, is released from apical suppression by the basal meristem. Interestingly, uniconazol, a gibberellin biosynthesis inhibitor, also caused a reduction in basal meristem activity, but without primary phyllomorph formation, suggesting that some gibberellin is required for proper function of the basal meristem. Co-application of gibberellin and cytokinin resulted in two macrocotyledons also without phyllomorph formation, which is similar to previous results for cytokinin-only applications. Thus, cytokinin may act downstream in the regulatory pathway of the basal meristem. Our results suggest that the balance between gibberellin and cytokinin in the cotyledons appears thus as key factor in the regulation of lateral and apical dominance in Streptocarpus. Their interplay may well be the primary explanation for the great diversity in growth form exhibited in species of this genus. Our work shows that small imbalances of hormones in early stages of plant development can have major effects on the final phenotype
The bending of cell sheets - from folding to rolling
The bending of cell sheets plays a major role in multicellular embryonic morphogenesis. Recent advances are leading to a deeper understanding of how the biophysical properties and the force-producing behaviors of cells are regulated, and how these forces are integrated across cell sheets during bending. We review work that shows that the dynamic balance of apical versus basolateral cortical tension controls specific aspects of invagination of epithelial sheets, and recent evidence that tissue expansion by growth contributes to neural retinal invagination in a stem cell-derived, self-organizing system. Of special interest is the detailed analysis of the type B inversion in Volvox reported in BMC Biology by Höhn and Hallmann, as this is a system that promises to be particularly instructive in understanding morphogenesis of any monolayered spheroid system
A Radiobrominated Tyrosine Kinase Inhibitor for EGFR with L858R/T790M Mutations in Lung Carcinoma.
Activating double mutations L858R/T790M in the epidermal growth factor receptor(EGFR) region are often observed as the cause of resistance to tyrosine kinase inhibitors (TKIs). Third-generation EGFR-TKIs, such as osimertinib and rociletinib (CO-1686), was developed to target such resistance mutations. The detection of activating L858R/T790M mutations is necessary to select sensitive patients for therapy. Hence, we aimed to develop novel radiobromine-labeled CO-1686 as apositron emission tomography (PET) imaging probe for detecting EGFR L858R/T790M mutations. Nonradioactive brominated-CO1686 (BrCO1686) was synthesized by the condensation of N-(3-[{2-chloro-5-(trifluoromethyl)pyrimidin-4-yl}amino]-5-bromophenyl) acrylamide with the corresponding substituted 1-(4-[4-amino-3-methoxyphenyl]piperazine-1-yl)ethan-1-one. The radiobrominated [77Br]BrCO1686 was prepared through bromodestannylation of the corresponding tributylstannylated precursor with [77Br]bromide and N-chlorosuccinimide. Although we aimed to provide a novel PET imaging probe, 77Br was used as an alternative radionuclide for 76Br. We fundamentally evaluated the potency of [77Br]BrCO1686 as a molecular probe for detecting EGFR L858R/T790M using human non-small-cell lung cancer (NSCLC) cell lines: H1975 (EGFR L858R/T790M), H3255 (EGFR L858R), and H441 (wild-type EGFR). The BrCO1686 showed high cytotoxicity toward H1975 (IC50 0.18 0.06 M) comparable to that of CO-1686 (IC50 0.14 0.05 M). In cell uptake experiments, the level of accumulation of [77Br]BrCO1686 in H1975 was significantly higher than those in H3255 and H441 upon 4 h of incubation. The radioactivity of [77Br]BrCO1686 (136.3% dose/mg protein) was significantly reduced to 56.9% dose/mg protein by the pretreatment with an excess CO-1686. These results indicate that the binding site of the radiotracers should be identical to that of CO-1686. The in vivo accumulation of radioactivity of [77Br]BrCO1686 in H1975 tumor (4.51 0.17) was higher than that in H441 tumor (3.71 0.13) 1 h postinjection. Our results suggested that [77Br]BrCO1686 has specificity toward NSCLC cells with double mutations EGFR L858R/T790M compared to those in EGFR L858R and wild-type EGFR. However, the in vivo accumulation of radioactivity in the targeted tumor needs to be optimized by structural modification
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