Comparison Of Fe(II) And Fe(III)-Hydralazine Complexes, A Potentiometric And Spectrophotometric Studa

Iron in oxidation states (+2 and +3) is very essential element for human body, and its concentration significantly altered in cardio vascular disease. So the aim of the present work is to study the interaction of Fe(II) and Fe(III) with very commonly used antihypertensive drug hydralazine through potentiometric and spectrophotometric methods. The objectives of the work is to study the stoichiometry, behavior of the complexes in aqueous solution, effect of pH and behavior of this drugs towards both oxidation states of Iron. Both methods show that hydralazine forms a stable complex with both oxidation states of the metal, but the nature of complex changes with change in pH, ligand concentration and with time span. Both methods confirms 1:2 stoichiometry for Fe(II)-Hydralazine while 1:3 for Fe(III)-Hydralazine. Stabilities of both complexes were also calculated. For Fe(II)-Hydralazine complex values of log β1 and log β2 were found to be 4.99 and  7.58 respectively. For Fe(III)-Hydralazine complex log β1, log β2 and log β3 values were found to be 2.74, 7.39 and 11.32 respectively. At high ligand concentration hydralazine also show reducing properties. The study suggests a strong interaction of hydralazine with iron; however the nature of interaction is different with both oxidation states of iron

Incidence and risk factors of intra uterine fetal death: a retrospective study at a tertiary care centre in Kashmir, India

Background: Intrauterine fetal death (IUFD) is the tragic event contributing to high perinatal mortality in developing countries. So many risk factors have been seen associated with IUFD that can be prevented with better antenatal care and timely detection at the earliest so that the prevalence can be decreased. This study was done to identify the risk factors associated with IUFD.Methods: This is a retrospective study from done from March 2017 to March 2018 at skims maternity hospital. IUFD was defined as fetal death beyond 20 weeks of gestation. Records were analyzed and data was compiled.Results: In our study there were total of 2500 deliveries out of which 70 were IUFD. Incidence was 28 per 1000 live births. It was found more common in the age group of 20-29 year (65.71%)  %). Preeclampsia was the risk factor in 17.14% of cases , followed by abruption in 11.42% followed by placenta previa in 7.14% of cases. However, 20% of the cases had unidentified risk factor.Conclusions: Present study was an effort to compile common risk factors associated with IUFD at tertiary centre of Kashmir

Stopped-Flow Kinetic Study of Reduction of Ferric Maltol Complex by Ascorbate

Stopped-flow kinetic investigation of reduction of Fe(III)-maltol complex is reported. The rates are dependent on pH in a complex way. On one hand at low pH there is a predominance of Fe(III)(maltol)2 which is easier to reduce compared to Fe(III) (maltol)3 which is more resistant to reduction. On the other hand ascorbate is a stronger reducing agent at higher pH. The rates are also found to be inversely dependent on the concentration of free ligand. These observations are explained by the following rate law:Rate = ((k0 +k1[H+])k2 [Asc-]/ (k-1[HMal] + k2[Asc-])) + k3 [Asc-] ) [FeIII(Mal)3] Here k1 is the rate constant for acid hydrolysis of the Fe(maltol)3 complex to Fe(maltol)2 complex and is directly controlled by H+, k0 is the rate constant for hydrolysis of the Fe(maltol)3 complex to Fe(maltol)2 complex and is an intrinsic process, k-1 is the rate constant of reformation of the tris complex by reaction of the bis complex and the free ligand, k2 is the rate constant for reduction of the bis complex by ascorbate and k3 is the rate constant for the reduction of the tris complex by ascorbate

Structural Modifications of KrF Excimer Laser-Ablated Zirconium Correlated to the Surface and Mechanical Properties

The structural modifications of KrF excimer laser-ablated zirconium (Zr) have been investigated in correlation with its surface and mechanical properties after ablation in deionized water and ethanol. KrF excimer laser of pulse duration of 20 ns, wavelength of 248 nm, and repetition rate of 20 Hz has been utilized for this purpose. Irradiation of Zr was carried out for varying number of laser pulses ranging from 500 to 2000 for laser fluence value of 3.6 J/cm2. The structural and chemical analyses were performed by using X-ray diffraction (XRD), Raman spectroscopy, and energy dispersive X-ray spectroscopy (EDS) techniques. Scanning electron microscope (SEM) and Vickers hardness tester were utilized for the analysis of surface morphology and hardness of laser-irradiated Zr targets. Presence of surrounding liquids played substantial role in structural, chemical, and mechanical modifications of Zr targets after irradiation. Pressure gradients and convective bubble motion owing to the confinement effects of the surrounding liquids, several thermal and chemical phenomena produced by heating through laser at the solid-liquid interface results in the generation of various hydrides and oxides of Zr, which are responsible for the development of various surface features and increase in hardness of irradiated Zr

Biodegradation of Sugarcane Trash with Agro-Industrial and Farm Wastes for Value Added Organic Byproduct

Arable land degradation, agro-waste management coupled with irrational use of chemicals has led to concern about soil and environmental health. The sugarcane crop generates lot of vegetative biomass after harvesting, which is burnt in field creating environmental implications. A research trail was conducted at green house premises department of soil and environment SAU Tandojam. The waste byproducts of the sugarcane trash was blended with pressmud, poultry and goat manure to have decomposed nutrient rich product subjected to 130 days decomposition/composting. Results revealed that the pH had significant relationship with organic substrate decomposition used for the compost preparation blended with sugarcane trash. The organic carbon loss or mineralization showed great variability and had relationship with the substrate used and decomposition time for a finished product. The more organic carbon (31.80%) was recorded for Cspm and (31.80%) C was found for Cpm. Compost product was rich in carbon potassium values corresponding (4.6%) for Cspm and (2.7%) K for Cgm. It was concluded that sugarcane trash can best be blended with sugarcane press mud of agro-industrial waste. Further it could be recycled with agro-industrial waste for waste management, reduce pollution, chemical fertilizers and used as organic soil amendment

Assessment of Risk to Healthcare Workers During the COVID-19 Pandemic: A Tertiary Care Facility Based Cross-sectional Study in Pakistan

Objective: To assess the risk of COVID-19 to healthcare workers (HCWs) in Tertiary care hospitals and its association with demographic factors. Study Design: Cross-sectional analytical study. Place and Duration of Study: Tertiary Care Hospital, Rawalpindi Pakistan, from May to Dec 2020. Methodology: Healthcare workers working in a designated COVID-19 Tertiary care hospital were included in the study. A modified "Risk assessment and management of exposure of HCWs in the context of COVID-19 tool" was distributed. HCWs were categorized as "high risk" and "low risk" of COVID-19 infection. Frequency and percentages were computed for demographic variables. Results: A total of 182 healthcare workers were included, and 167(91.7%) returned the study questionnaire. Most of them were nurses (n=65, 40.1%) working in the medical unit (n=99, 61.1%). Low risk HCWs were 73.5%( n=119) and only 26.5%( n=43)were high risk. Gender (p-value: .02) and type of HCWs (p-value: .01) were significantly associated with the risk of COVID-19. Conclusion: One-fourth of HCWs were at high risk of COVID-19 virus infection. Female gender and nurses were more likely to acquire COVID-19 infection

A novel pH-responsive hydrogel system based on Prunus armeniaca gum and acrylic acid: Preparation and evaluation as a potential candidate for controlled drug delivery

pH-responsive hydrogels have become effective and attractive materials for the controlled release of drugs at pre-determined destinations. In the present study, a novel hydrogel system based on Prunus armeniaca gum (PAG) and acrylic acid (AA) was prepared by a free radical mechanism using N, N-methylene bisacrylamide (MBA) as cross-linker and potassium persulfate (KPS) as initiator. A series of hydrogels varying PAG, AA, and MBA concentration was developed to determine the impact of these components. Formulated hydrogels were characterized for pH-responsive swelling, drug release, gel content, and porosity. Structural analysis was performed by FTIR, XRD, and SEM analysis. TGA study was applied to assess thermal stability. Oral acute toxicity and in vivo drug release were performed in rabbits. Hydrogels exhibited pH-dependent swelling and drug release. Swelling, drug loading and release, and porosity increased by increasing PAG and AA concentration while decreased by increasing MBA. The gel content of formulations was increased by increasing all three components. FTIR studies confirmed the development of copolymeric networks and the loading of drug. XRD studies revealed that hydrogels were amorphous, and the crystalline drug was changed into an amorphous form during loading. TGA results indicated that hydrogels were stable up to 600 °C. Acute oral toxicity results confirm that hydrogels were nontoxic up to a dose of 2 g/kg body weight in rabbits. The pharmacokinetic evaluation revealed that hydrogels prolonged the availability of the drug and the peak plasma concentration of the drug was obtained in 6 h as compared to the oral solution of the drug. Tramadol hydrochloride (THC) was used as a model drug. Hence, pH-responsive swelling and release, nontoxic nature and improved pharmacokinetics support that PAG-based hydrogels may be considered as potential controlled-release polymeric carriers

Synthesis, Characterization, Biological Activities and Ab-initio Study of Transition Metal Complexes of [Methyl 2-((4-chlorophenyl)(hydroxy)methyle) Acrylate]

Taking cognizance of the medicinal significance and diverse functions of synthetic Morita-Baylis-Hillman adducts (MBHA), the title ligand was synthesized and purified through column chromatography. Cr+3, Mn+2, Co+3, Ni+2, Cu+2 complexes of the ligand were synthesized under basic conditions, subjected to characterization through spectral analyses and verified with the IR spectrum that was generated computationally by the DFT B3LYP method, with 6-311++ G (d,p) basis set and Hartree Fock (HF) B3LYP method in conjunction with 3-21G(d,p) basis set. Powder XRD helped to testify crystals of the complexes. Moreover, the antibacterial, and antioxidant characteristics of MBHA and its complexes were also established. All of them were found to be active antioxidants. The antibacterial activities, examined against S. aureus, E. coli, B. pumilis and S. typhi have revealed that its Cobalt complex has an excellent potential to act against all of them. Hence, these compounds maybe having potentialities for the discovery of new, cheaper and efficient drugs against various infectious diseases. The study also uncovers the first example of utilization of MBHA towards metal complex formation

Revealing Genome-Based Biosynthetic Potential of Streptomyces sp. BR123 Isolated from Sunflower Rhizosphere with Broad Spectrum Antimicrobial Activity

Actinomycetes, most notably the genus Streptomyces, have great importance due to their role in the discovery of new natural products, especially for finding antimicrobial secondary metabolites that are useful in the medicinal science and biotechnology industries. In the current study, a genomebased evaluation of Streptomyces sp. isolate BR123 was analyzed to determine its biosynthetic potential, based on its in vitro antimicrobial activity against a broad range of microbial pathogens, including gram-positive and gram-negative bacteria and fungi. A draft genome sequence of 8.15 Mb of Streptomyces sp. isolate BR123 was attained, containing a GC content of 72.63% and 8103 protein coding genes. Many antimicrobial, antiparasitic, and anticancerous compounds were detected by the presence of multiple biosynthetic gene clusters, which was predicted by in silico analysis. A novel metabolite with a molecular mass of 1271.7773 in positive ion mode was detected through a high-performance liquid chromatography linked with mass spectrometry (HPLC-MS) analysis. In addition, another compound, meridamycin, was also identified through a HPLC-MS analysis. The current study reveals the biosynthetic potential of Streptomyces sp. isolate BR123, with respect to the synthesis of bioactive secondary metabolites through genomic and spectrometric analysis. Moreover, the comparative genome study compared the isolate BR123 with other Streptomyces strains, which may expand the knowledge concerning the mechanism involved in novel antimicrobial metabolite synthesis

Revealing genome-based biosynthetic potential of Streptomyces sp. BR123 isolated from sunflower rhizosphere with broad spectrum antimicrobial activity

Actinomycetes, most notably the genus Streptomyces, have great importance due to their role in the discovery of new natural products, especially for finding antimicrobial secondary metabolites that are useful in the medicinal science and biotechnology industries. In the current study, a genome-based evaluation of Streptomyces sp. isolate BR123 was analyzed to determine its biosynthetic potential, based on its in vitro antimicrobial activity against a broad range of microbial pathogens, including gram-positive and gram-negative bacteria and fungi. A draft genome sequence of 8.15 Mb of Streptomyces sp. isolate BR123 was attained, containing a GC content of 72.63% and 8103 protein coding genes. Many antimicrobial, antiparasitic, and anticancerous compounds were detected by the presence of multiple biosynthetic gene clusters, which was predicted by in silico analysis. A novel metabolite with a molecular mass of 1271.7773 in positive ion mode was detected through a high-performance liquid chromatography linked with mass spectrometry (HPLC-MS) analysis. In addition, another compound, meridamycin, was also identified through a HPLC-MS analysis. The current study reveals the biosynthetic potential of Streptomyces sp. isolate BR123, with respect to the synthesis of bioactive secondary metabolites through genomic and spectrometric analysis. Moreover, the comparative genome study compared the isolate BR123 with other Streptomyces strains, which may expand the knowledge concerning the mechanism involved in novel antimicrobial metabolite synthesi