Methods of DNA methylation detection

The present invention provides for methods of DNA methylation detection. The present invention provides for methods of generating and detecting specific electronic signals that report the methylation status of targeted DNA molecules in biological samples.Two methods are described, direct and indirect detection of methylated DNA molecules in a nano transistor based device. In the direct detection, methylated target DNA molecules are captured on the sensing surface resulting in changes in the electrical properties of a nano transistor. These changes generate detectable electronic signals. In the indirect detection, antibody-DNA conjugates are used to identify methylated DNA molecules. RNA signal molecules are generated through an in vitro transcription process. These RNA molecules are captured on the sensing surface change the electrical properties of nano transistor thereby generating detectable electronic signals

Biorenewable materials for water remediation:The central role of cellulose in achieving sustainability

As the population increases and manufacturing grows, greenhouse gas and other harmful emissions increase. Contaminated with chemicals such as dyes, pesticides, pharmaceuticals, oil, heavy metals or radionuclides, wastewater purification has become an urgent issue. Various technologies exist that can remove these contaminants from wastewater sources, but they often demand high energy and/or high cost, and in some cases produce contaminant laden sludge that requires safe disposal. The need for methods which are less capital intensive, less operationally costly and more environmentally friendly is suggested. Cellulose-based materials have emerged as promising candidates for wastewater treatment due to their renewability, low cost, biodegradability, hydrophilicity, and antimicrobial property. In this review article, we focussed on developing sustainable and biodegradable cellulose-based materials for wastewater treatment. This article deals with cellulose-based materials’ scope and their conversion into valuable products like hydrogel, aerogel, cellulose composites, and nanocellulose. The cellulose-based materials have no harmful environmental impact and are plentiful. The modified cellulose-based materials applying as membrane, adsorbent, sorbent, and beads to purify the wastewater were discussed. Finally, the challenges and future prospects of cellulose-based materials for wastewater treatment were considered, emphasizing their potential to be sustainable and eco-friendly alternatives to traditional materials used in wastewater treatment.</p

Electrochemical studies on somesynthesised N-sulphonamoylphenyl- 3-aminophenyl- 5-methyl-4-[ 4'(2"-pyrimidinyl)sulphonamoyl]- phenylazopyrazoles

449-452Present note deals with the electrochemical behaviour of N-sulphonamoylphenyl-3-aminophenyl-5methyl- 4 -[4'(2"-pyrimidinyl)sulphonamoyl]-phenylazopyrazoles. All the studies have been carried out in DMF-water admixture using Britton-Robinson buffers of varying pH. A single, well defined, diffusion-controlled reduction wave is obtained which shifted towards more negative potential with increase in the pH. On the basis of number of protons consumed and electron transferred, a plausible mechanism has also been suggested