The modular method: Milkfish pond culture

The modular method of milkfish culture (Chanos chanos) described in the manual is an improvement over the traditional extensive method. The manual is intended for the use of fish farmers and aquaculturists, extensionists, and students of aquaculture not only in the Philippines, but also in other milkfish-producing countries in Southeast Asia and the world. It covers the following: Interesting facts about milkfish -- biological characteristics, artificial breeding of milkfish; Design and operation of modular pond system -- pond preparation, stocking in the nursery or transition ponds, stocking in the rearing ponds, care of stock, pond utilization and production schedule, harvest and post-harvest; and, Economics and costing

Mudcrab, Scylla spp, production in brackishwater ponds

This manual covers the specifics of grow-out operation — site selection, pond specification, pond preparation, source of juveniles, transport and stocking, care of pond and stock, feeds and feeding, harvest, postharvest. Also includes costs-and-benefits analysis and a list of useful references.Mudcrab (Scylla spp) production in brackishwater ponds is now gaining popularity, especially in communities that need to supplement their income. The manual covers the following: Distribution; Grow-out operation in ponds - site selection, pond specification, pond preparation, source of juveniles, transport and stocking of juveniles, care of pond and stock, feeds and feeding, harvest, post-harvest; Production and profits; Cost and analysis. It is hoped that the manual will be of use to fishfarmers and aquaculturists, extensionists, and students of aquaculture not only in the Philippines but also in other mudcrab producing countries in Southeast Asia

Grouper culture in floating net cages

The manual describes the culture of groupers (Epinephelus) in floating cages, providing a farming option for grouper growers and also a production alternative to the farmed species being done today, such as shrimp, milkfish and tilapia. The following aspects are covered: species identification for commercially cultured groupers; source of stock; net cage specifications; anchor; hides and shelters; nursery net cage operation; production cages; harvesting; post-harvest; profitability analysis of grouper cage culture; and, cost and return of growing grouper in cages

Perancangan Cd Katalog untuk Penyampaian Layanan Informasi Berbasis Multimedia pada Rumah Sakit Persahabatan

CD katalog developed is an effort to improve service quality and effectiveness of delivering information to visitors Persahabatan Hospital. The method used is the method of literature, methods of analysis and design methods. Methods Literatur include the study of literature references relating to problems and applications. Methods include analysis of field studies with a survey on the current system as well as an analysis of surveys, interviews, and questionnaire distribution. The design method includes designing the menu structure, State Transition diagrams, screen design, database use, and module specifications. The results obtained in the form of multimedia CD-based catalog that presents the completeness of the information, such as facilities Persahabatan Hospital, clinic, the site plan, and schedule a doctor. The conclusion was based on a multimedia CD Catalog can be a new alternative as a medium to facilitate the search for location information and profile hospital. With the CD catalog is expected to visitors and prospective participants can find information about the Friendship Hospital, more easily, and help the Friendship Hospital in providing information to visitors Hospitals

Efficacy of Perampanel in Refractory and Super-Refractory Status Epilepticus with Suspected Inflammatory Etiology: A Case Series

(1) Background: Increasing evidence supports the anti-inflammatory and neuroprotective role of perampanel (PER), mediated by decreased expression of pro-inflammatory cytokines and by interference with apoptosis processes. Therefore, the use of PER to treat status epilepticus (SE) with suspected inflammatory etiology is appealing and deserves further investigation. (2) Methods: We retrospectively analyzed seven patients (five F, two M; median age: 62 years) with refractory and super-refractory SE due to a probable or defined inflammatory etiology and treated with PER. (3) Results: PER was administered as the third (4/7) or fourth drug (3/7), with a median loading dose of 32 mg/day (range: 16–36 mg/day) and a median maintenance dose of 10 mg/day (range: 4–12 mg/day). In five cases, SE was focal, while in two patients, it was generalized. SE was caused by systemic inflammation in three patients, while in the other four subjects, it was recognized to have an autoimmune etiology. SE resolution was observed after PER administration in all cases, particularly within 24 h in the majority of patients (4/7, 57.1%). (4) Conclusions: Our data support the efficacy of PER in treating SE when first- and second-line ASMs have failed and suggest a possible earlier use in SE cases that are due to inflammatory/autoimmune etiology

Low X-Ray Luminosity Galaxy Clusters: Main goals, sample selection, photometric and spectroscopic observations

We present the study of nineteen low X-ray luminosity galaxy clusters (L$_X \sim$ 0.5--45 $\times$ $10^{43}$ erg s$^{-1}$), selected from the ROSAT Position Sensitive Proportional Counters (PSPC) Pointed Observations (Vikhlinin et al. 1998) and the revised version of Mullis et al. (2003) in the redshift range of 0.16 to 0.7. This is the introductory paper of a series presenting the sample selection, photometric and spectroscopic observations and data reduction. Photometric data in different passbands were taken for eight galaxy clusters at Las Campanas Observatory; three clusters at Cerro Tololo Interamerican Observatory; and eight clusters at the Gemini Observatory. Spectroscopic data were collected for only four galaxy clusters using Gemini telescopes. With the photometry, the galaxies were defined based on the star-galaxy separation taking into account photometric parameters. For each galaxy cluster, the catalogues contain the PSF and aperture magnitudes of galaxies within the 90\% completeness limit. They are used together with structural parameters to study the galaxy morphology and to estimate photometric redshifts. With the spectroscopy, the derived galaxy velocity dispersion of our clusters ranged from 507 km~s$^{-1}$ for [VMF98]022 to 775 km~s$^{-1}$ for [VMF98]097 with signs of substructure. Cluster membership has been extensively discussed taking into account spectroscopic and photometric redshift estimates. In this sense, members are the galaxies within a projected radius of 0.75 Mpc from the X-ray mission peak and with cluster centric velocities smaller than the cluster velocity dispersion or 6000 km~s$^{-1}$, respectively. These results will be used in forthcoming papers to study, among the main topics, the red cluster sequence, blue cloud and green populations; the galaxy luminosity function and cluster dynamics.Comment: 13 pages, 6 tables, 9 figures. Uses emulateapj. Accepted for publication in The Astronomical Journal. Some formatting errors fixe

Negative selection and stringency modulation in phage-assisted constinuous evolution

Phage-assisted continuous evolution (PACE) uses a modified filamentous bacteriophage life cycle to dramatically accelerate laboratory evolution experiments. In this work we expand the scope and capabilities of the PACE method with two key advances that enable the evolution of biomolecules with radically altered or highly specific new activities. First, we implemented small molecule-controlled modulation of selection stringency that enables otherwise inaccessible activities to be evolved directly from inactive starting libraries through a period of evolutionary drift. Second, we developed a general negative selection that enables continuous counter-selection against undesired activities. We integrated these developments to continuously evolve mutant T7 RNA polymerase enzymes with ∼10,000-fold altered, rather than merely broadened, substrate specificities during a single three-day PACE experiment. The evolved enzymes exhibit specificity for their target substrate that exceeds that of wild-type RNA polymerases for their cognate substrates, while maintaining wild-type-like levels of activity

Can epilepsy affect normal EEG variants? A comparative study between subjects with and without epilepsy

Objectives: To compare the prevalence of benign EEG variants (BEVs) between epileptic and non-epileptic subjects. Methods: A prospective, observational EEG study of 1,163 consecutive patients, using the 10–20 international system with systematically two additional anterior/inferior temporal electrodes. The video-EEG monitoring duration was between 24 h and eight days. Results: We identified 917 (78.9%) epileptic patients (mean age: 33.42 ± 15.5 years; females: 53.4%) and 246 (21.2%) non-epileptic patients (mean age: 35.6 ± 18.75 years; females: 54.9%). Despite a shorter mean duration of the EEG recordings, the prevalence of BEVs was higher in non-epileptic vs. epileptic patients (73.2% vs. 57.8%, p = 0.000011). This statistical difference was confirmed for lambda waves (23.6% in the non-epilepsy group vs. 14.8% in the epilepsy group, p = 0.001), POSTs (50.8% vs. 32.5%, p < 0.000001), wicket spikes (20.3% vs. 13.6%, p = 0.009) in particular in NREM and REM sleep, and 14- and 6-Hz positive bursts (13% vs. 7.1% p = 0.003). Mu rhythm was observed at the same frequency in both groups (21.1% in the non-epilepsy group vs. 22.7% in the epilepsy group). There was no difference between the two groups for rarer rhythms, such as rhythmic mid-temporal theta burst of drowsiness, small sharp spikes, and midline theta rhythm. Conclusions: There was no increase in any of the BEVs in the epilepsy group. On the contrary, BEVs were more frequent and diversified in the non-epilepsy group. Epilepsy may negatively affect the occurrence of the most common BEVs, with the exception of the mu rhythm, which is present in about one-fifth of the population with or without epilepsy

Properties of the Visible Light Phototaxis and UV Avoidance Behaviors in the Larval Zebrafish

For many organisms, color is an essential source of information from visual scenes. The larval zebrafish has the potential to be a model for the study of this topic, given its tetrachromatic retina and high dependence on vision. In this study we took a step toward understanding how the larval zebrafish might use color sensing. To this end, we used a projector-based paradigm to force a choice of a color stimulus at every turn of the larva. The stimuli used spanned most of the larval spectral range, including activation of its Ultraviolet (UV) cone, which has not been described behaviorally before. We found that zebrafish larvae swim toward visible wavelengths (>400 nm) when choosing between them and darkness, as has been reported with white light. However, when presented with UV light and darkness zebrafish show an intensity dependent avoidance behavior. This UV avoidance does not interact cooperatively with phototaxis toward longer wavelengths, but can compete against it in an intensity dependent manner. Finally, we show that the avoidance behavior depends on the presence of eyes with functional UV cones. These findings open future avenues for studying the neural circuits that underlie color sensing in the larval zebrafish