Sexual reproduction cycle of the sea cucumber (Stichopus hermanni) in the coral reefs of Kish Island of Iran

Sea cucumber is commercially important in the world. Reproductive biology of Stichopus hermanni in the coral reefs of the Kish Island was investigated using 252 specimens. Morphology of the gonad of the sea cucumber species of the Kish Island was found to be similar to other species. We showed that the gonad color is an unreliable character for sex determination except in maturity stage. We reported the gametogenesis sequence of events and found a sex ratio of 1:1 for the species. The sequence begins late in winter and continues until summer. The active stage of gametogenesis coincides with increasing photoperiod and temperature. Very little spawning activity out of season was noticeable and the maximum spawning was seen in summer. Average body length at first maturity was found to be 310 mm and the diameter of mature oocytes was 200 um. Oocytes moved upward at a rate of 20-30mm per minute that confirms divers' reports of the abundance of developing S. hermanni embryos near the water surface

Perencanaan & Penganggaran Bagi Pengelolaan Hutan Berbasis Masyarakat Di Indonesia

The weak commitment of central and local government to community forest management is seen in the poor planning and budget allocation for community forestry schemes (HKm), village forests (HD), and community forests (HTR) at ground level. To solve this problem, Kemitraan has studied the planning and budget allocation for community-based forest management, especially HKm, HD and HTR both at national and regional level. The study examines four provinces : Jambi, West Kalimantan, West Nusa Tenggara, and South East Sulawesi, as well as the coordination between central and local governments, presenting policy recommendations to address problems and sucessfully implement social forestry acceleration in Indonesia

Local Knowledge and the Adoption of Science Knowledge in Cocoa Cultivation Community in East Kolaka Regency

This study aims to analyze the existence of local farming knowledge by cacao farmers community and their integration with science knowledge from outside.The results showed that local knowledge in cacao cultivation is based on customs and traditions as well as the insistence of family life which has been the custom of farmers and then processes through repetitions which then form a farming experience. Science knowledge in cacao cultivation is formed based on the response to the decline in productions and user preferences and the inovation in farming technology which is introduced to users through technology transfer by researchers, extension agents and plantation assistants. The acceleration of technological innovation by the government was not followed by the speed and utilization of science knowledge by cacao farmers, and the weaknesses are in the delivery subsystem and the recipient subsystem. It takes a continuous bridge between research institutions as suppliers of science technology/ knowledge with their users, so that the resulting science knowledge is guaranteed to be followed by users on an ongoing basis

Hydrology and hydrobiology and environmental pollutions in lower than 10 meters depths of Caspian Sea

The present project in related to survey of factors and hydrology and hydrochemical features (water temperature, dissolve oxygen saturation, pH, clearance, salinity, nitrogen, phosphorus and silicon) hydrobiology (zooplankton, phytoplankton, macrobenthos) and survey of bio environment pollution (oil, heavy metal, detergent) executed in lower 10m in different water larger in southern Caspian Sea in 2002-2003. For sampling 8 lines number were vertical on coast that selected from Astra in west to Gomishan in east in southern Caspian Sea basin. The result indicated the average physical factors such as pH were 8.11 and salinity12.12 ppt ,and disolve oxygen6.7 mg/l. Average chemical factors such as NO_2 , NO_3 and NH_4 were 1.2 µg/l, 25.7 µg/l, 13 µg/l respectively.Total nitogen and organic nitrogen and inorganic nitrogen were 690.2 µg/l , 667.6 µg/l , 41.6 µg/l. Average silicat were recrded 266.35 µg/l . Total Phosphorus was observed 37.35 µg/l and average of organic Phosphorus concentration was and 20.25 µg/l .Average of Total organic matter (T.O.M) was 4.98% maximum amount were observed in Lisar and minimum in Nooshahr . Concentration of heavy metal during sampling were respectively ,Fe>Mn>Zn>Cr>Ph>Co>Cd>Cu. Maximum concentration of Fe were determined in winter in Nooshahr and Babolsar respectively 13/3 µg/l 17/1 µg/l. In many stations and different Season, the amount of heavy meta were lower standard of in marine water. The concentration of oil hydrocarborate (PAHs) in autumn was 0/13 ppb and in winter 0/12 ppb. The amount of (PAHs) in Southern Caspian Sea were Lower than other parts of Caspian Sea. The average of detergent concentration (LAS) was 0/036 µg/l that was two fold higher than determined in 2001. Total 107 species of phytoplankton belong to 5 phylum were identified. The numbers of species of phytoplankton groups were respectively, chrysophyta (42 species), cyanophyta (17 species), pyruphyta (17 species), chlorophyta (21 species) and euglenophyta (9 species). The maximum diversity of phytoplankton observed in summer and minimum in autumn. High diversity of chrysophta and cyanophyta observed in summer and phyrophyta and chrlophyta in spring. The composition of phytoplanhkton groups were respectively, chrysophyta (70%), phyrophyta (9%) and chlorophyta (7%) and euglonophyta (1%). Maximum density of phytoplankton was observed in autumn and minimum in winter. Total 19 species of Zooplankton were identified. Maximum diversity was observed in summer and minimum in winter. Zooplankton changes during sampling, showed amount of density of zooplankton in 5m were more than 10 m depths. Total (17 species macrobenthos were identified. The composition of macrobenthos groups were respectively , Annalida (92/7% ) , Bivalvia (2/7%) gumarida (108%) cumacea ( 1/5%) , Balanidae 103% . max . density were observed in Astara and min . in Sefied roud Average of density were 1218 0/851 ind /m^2 and biomass 14 15 g/m^2 High density were recorded in autumn and low density in winter . Correlation of phytoplankton and zooplankton with physicochemical parameter and also relation between total organic matter and sediment grain size were calculated.Ecological indicies (simpson diversity evenns diversity and shanoon-wiever diversity) were calculated for macrobenthos. Data were shown impact of cetenephora (Mnenemiopsis leidyi) on zooplankton and phytoplankton and macrobenthos density

Development and Validation of Simple RP-HPLC Method for Intracellular Determination of Fluconazole Concentration and Its Application to the Study of Candida albicans Azole Resistance

Candida albicans (strains NCTC-885-653 and ATCC-10231) long-term cultivated in the presence of antifungal agent fluconazole (FLC) and classical microbiological methods for determination of minimal inhibitory concentration (MIC) were used in this study. A simple and sensitive method based on reverse-phase high-performance liquid chromatography (RP-HPLC) has been developed for the determination of FLC intracellular concentration in C. albicans using tinidazole as an internal standard. Following extraction with dichloromethane, the chromatographic separation was achieved on a Machery-Nagel EC250/2 Nucleodur-100-3 C18 column by gradient elution using the mobile phase consisting of (A) 0.01 M ammonium acetate buffer, pH = 5.00, and (B) acetonitrile. Different analytical performance parameters such as linearity, precision, accuracy, limit of quantification (LOQ), and robustness were determined according to US DHHS FDA and EMEA guidelines. The method was linear for FLC (r=0.9999) ranging from 100 to 10000 ng/mL. The intraday and interday precisions (relative standard deviation) were within 2.79 and 2.64%, respectively, and the accuracy (relative error) was less than 2.82%. The extraction recovery ranged from 79.3 to 85.5%. The reliable method was successfully applied to C. albicans azole-resistance study and it was shown that intracellular concentration of FLC correlated with a yeast drug susceptibility profile and MIC values

Anemia outcome after laparascopic mini bypass: Analysis of 107 consecutive patients

Background: The prevalence of obesity has dramatically increased globally. Weight loss procedures are known to be an effective and reliable method with relatively low complication rate and satisfactory results. Laparoscopic mini-gastric bypass (LMGB) is known as a modified Mason loop procedure with compatible results to laparoscopic Roux-en-Y gastric bypass (LRYGB), and is believed to have even less complication rate. Despite adequate and meticulous supplement therapy, anemia is one of the challenges in patients undergoing LMGB. Thus, we aimed to review the prevalence and severity of anemia in patients undergoing LMGB. Method: A prospectively-maintained database of patients referring to Hazrat Rasoul Akram hospital who underwent LMGB from December 2013 to October 2014 was reviewed retrospectively. Results: A total of 113 consecutive patients were included in the study. The mean age was 38.7 ± 9.8 years. Mean Body mass index (BMI) was 45.5 ± 6.1 kg/m2 preoperatively and 36.7 ± 5.5 kg/m2 and 33.0 ± 5.3 kg/m2 three and six months after the procedure, respectively. (P = 0.001) Mean excess body weight loss after the procedure was 20 and 53 at three and six months post operatively. Serum hemoglobin (Hb) level decreased significantly in three months (P = 0.036) and remained unchanged in six months compared to 3-month (P = 0.385). Vitamin B12 level increased significantly in three months (P = 0.010) and then decrease in six months to the preoperative level (P = 0.889). Conclusion: LMGB is a safe, feasible and an effective alternative weight loss procedure. Simply, anemia can be prevented by utilizing therapeutic dose of multivitamin in patients who underwent this procedure. © 2016, Universa Press. All rights reserved