Variability of Physiological Parameters of European Beech Provenances in International Provenance Trials in Serbia

In this study, the variability of physiological parameters of five provenances of Europeanbeech (Fagus sylvatica), which were planted at two locations with different ecological conditions atFruška Gora and Debeli Lug, was estimated. Provenance trials were established in the framework ofCOST Action E52: "Evaluation of Beech Genetic Resources for Sustainable Forestry". 2-3 years oldseedlings originating from Croatia, Germany, Bosnia, Austria and Serbia were planted in blocks offifty plants with a spacing of 2 x 1 m. Physiological parameters such as net photosynthesis, rate oftranspiration and stomatal conductance were measured with a portable gas analysis system. Generally,provenances from Fruška Gora Mountain showed higher intensity of all physiological parameters thanprovenances located at site Debeli Lug. High correlations among rates of net photosynthesis andtranspiration, on one side, and stomatal conductance, on the other side, were found. ANOVA testindicates that variability of net photosynthesis, transpiration and stomatal conductance of investigatedprovenances, at the two locations, was influenced both by environmental conditions of sites andgenetic constitution of provenances

Efekat primene polifosfatnog stakla u supstratima za gajenje cveća i povrća

Due to the dissolution properties the phosphate glasses can be promising materials for the development of new ecologically safe fertilizers. The complex polyphosphate glass with a molar ratio (M2O + MO)/P2O5 >1 and addition of microelements Zn2+, Mn2+, Fe3+ and Cu2+ were used in substrate for production of flower and vegetables seedlings. Dissolution tests were conducted in deionized water and 2% citric acid. The effects of the reaction time on the normalized glass mass loss (fm) and the normalized concentrations of the ions (fi) at 20, 30, 37 and 50°C were determined. Dissolution rate constants (kw - deionized water, kc -2% citric acid) were also determined (kw-0.0028-0.0094 gm-2, kc-0.029-0.011 gm-2). Values of kc were higher than those of kw, which confirms a significant effect of solvent type and temperature on the dissolution kinetics of glass. The experiments with plants (one vegetable and one flower) showed that the addition of phosphate glass caused an increase in yield and quality of plants. The applied dose of polyphosphate glass of 1gl-1 showed the best effect on the growth of the examined plant seedlings.Polifosfatno staklo, u kome je molski odnos (M2O + MO)/P2O5 >1, koje sadrži i mikroelemente Zn2+, Mn2+, Fe3+ i Cu2+, korišćeno je u supstratu za proizvodnju rasada cveća i povrća. Eksperimenti su izvedeni u stakleniku na Poljoprivrednom fakultetu u Beogradu i u laboratorijama ITNMS. Ispitivano je ponašanje polifosfatnog stakla prilikom rastvaranja u dejonizovanoj vodi i 2% rastvoru limunske kiseline na temperaturama od 20, 30, 37 i 50 °C. Ispitan je uticaj vremena rastvaranja na normalizovani gubitak mase (fm) i normalizovane koncentracije jona (fi). Određene su i konstante brzina rastvaranja(kw-dejonizovana voda, kc -2% rastvor limunske kiseline). Vrednosti kc su veće od vrednosti za kw, što potvrđuje uticaj rastvarača i temperature na proces rastvaranja. Rezultati su pokazali pozitivan efekat polifosfatnog stakla na rast i kvalitet ispitivanih biljaka. Primenjena doza polifosfatnog stakla od 1 gl-1 pokazala je najbolji efekat na rast ispitivanih biljaka-rasada

Efekti korišćenja sirove soje u obrocima krava u prvih 100 dana laktacije

The results of using ground soybean grain in feeding lactating cows were shown. The inclusion of 1-2 kg ground soybean grain in diets for cows increased milk amount for 1.76 kg/cow per day and 636 kg/cow per year. The results show that the crude soybean grain has positive influence on milk production and hasn't negative influence on health status cows. .U radu su prikazani rezultati korišćenja prekrupljenog sirovog zrna soje u ishrani krava u laktaciji. Uključivanjem 1-2 kg (zavisno od količine mleka) prekrupljenog sirovog zrna soje u obrok krava došlo je do povećanja količine mleka za 1,76 kg/krava dnevno, odnosno za 639 kg/krava godišnje. Rezultati pokazuju da prekrupljeno sirovo zrno soje ima pozitivan uticaj na produkciju mleka i pri tome nema negativnog uticaja na zdravstveno stanje krava.

Efekti tova junadi do različitih završnih masa

In this work the results about fattening young bulls along with influence different factors were exposed. Especially, the basic factors binding with success of fattening young bulls (different starting and final weights, sex, composite of diet) are given. The results binding with growth, composite of tissue, increasing of proteins and fat with different final weights at butcher are especially given. .U radu su izloženi brojni rezultati u oblasti tova junadi uz uticaj različitih faktora. Posebno su istaknuti osnovni faktori od kojih u velikom stepenu zavisi uspeh tova (različite početne i završne telesne mase junadi, polna pripadnost grla, sastav obroka). U domenu efekata posebno su izneti rezultati vezani za prirast, sastav tkiva, povećanja proteina i masti pri različitim telesnim masama pri klanju i td

Utjecaj ekspozicije krošnje na morfološka svojstva iglica devet četinjača

The aim of this research was to investigate if the crown exposure of some conifers influenced to needle properties. The leaf morphological traits of sixty-six trees of nine conifers: Atlas cedar, Austrian pine, Blue spruce, Douglas fir, European spruce, European yew, Serbian spruce, Silver fir, and White fir, from six Belgrade parks, were analyzed. Five needles were measured from each of the four main crown exposures. Length, width, area and perimeter of needles were investigated. Species, parks in which they were found, as well as crown exposures, differed mostly in needle length and needle width. Correlations between measured needle traits were determined by linear regression analysis. Strong positive correlations were found between the length, perimeter, and area of needles. The differences among the species in terms of light requirement determine species for individual planting as light-loving or partial shade species (Atlas cedar, European spruce, Serbian spruce, Blue spruce, Austrian pine and Douglas fir), or for group planting as shade-loving species (Silver fir, White fir and European yew).Analizirano je 66 stabala devet vrsta četinjača: atlaskog cedra, crnog bora, bodljikave smreke, duglazije, obične smreke, šumske tise, Pančićeve omorike, koloradske  jele i obične jele, iz šest beogradskih parkova. Analizirano je pet iglica sa svake od četiri glavne ekspozicije krošnje. Ispitivana je duljina, širina, površina i opseg iglica. Vrste, parkovi u kojima su pronađene, kao i ekspozicije njihovih kruna, razlikovali su se po duljini i širini iglica. Korelacije između izmjerenih svojstava iglica određene su linearnom regresijskom analizom. Utvrđene su jake pozitivne korelacije između duljine, opsega i površine iglica. Razlike među vrstama u zahtjevima za svjetlom određuju vrste za pojedinačnu sadnju kao vrste koje vole svjetlo ili polusjenu (atlaski cedar, obična smreka, Pančićeva omorika, bodljikava smreka, crni bor i duglazija) ili za grupnu sadnju kao sjenoljubne vrste (obična jela, dugoigličava jela i šumska tisa)

Inflammatory Cytokines Prime Adipose Tissue Mesenchymal Stem Cells to Enhance Malignancy of MCF-7 Breast Cancer Cells via Transforming Growth Factor-beta 1

Mesenchymal stem cells from human adipose tissue (hASCs) are proposed as suitable tools for soft tissue engineering and reconstruction. Although it is known that hASCs have the ability to home to sites of inflammation and tumor niche, the role of inflammatory cytokines in the hASCs-affected tumor development is not understood. We found that interferon-gamma (IFN-gamma) and/or tumor necrosis factor-alpha (TNF-alpha) prime hASCs to produce soluble factors which enhance MCF-7 cell line malignancy in vitro. IFN-gamma and/or TNF-alpha-primed hASCs produced conditioned media (CM) which induced epithelial to mesenchymal transition (EMT) of MCF-7 cells by reducing E-Cadherin and increasing Vimentin expression. Induced EMT was accompanied by increased invasion, migration, and urokinase type-plasminogen activator (uPA) expression in MCF-7 cells. These effects were mediated by increased expression of transforming growth factor-beta 1(TGF-beta 1) in cytokines-primed hASCs, since inhibition of type I TGF-beta 1 receptor on MCF-7 cells and neutralization of TGF-beta 1 disabled the CM from primed hASCs to increase EMT, cell migration, and uPA expression in MCF-7 cells. Obtained data suggested that IFN-gamma and/or TNF-alpha primed hASCs might enhance the malignancy of MCF-7 cell line by inducing EMT, cell motility and uPA expression in these cells via TGF-beta 1-Smad3 signalization, with potentially important implications in breast cancer progression

The possibility of maize gluten application for weed control in maize and soybean

The objective of this study was to determine the effects of maize gluten on the weeds number and dry biomass in the maize and soybean experiment. Pre-emergence maize gluten application resulted with an effective weed control in maize, whereas the soybean had the significant loss of plants, due to the gluten fitotoxicity. Post-emergence application was found to be less successful compared with pre-emergence application particularly for soybean. Panicum cruss-galli L. was the most frequent weed in the both experiment. Maize gluten rate of 300 g m2 could be recommended in control of the broad leaf weeds in maize cropping as an alternative herbicide thus a substitute for mineral nitrogen. Our research can contribute to the improvement of the weed control in sustainable cropping systems

Vibration analyzing in horizontal pumping aggregate by soft computing

The main goal of the study was to analyze vibration of pumping aggregate. There are fourth position which could be very harmful for the total working operation of the pumping aggregate. The pumping aggregated should have smooth continuous operations without any mistake. Vibration could affect different parts or segments of the pumping aggregate and therefore it is need to analyze the vibrating. Analyzing of the vibration could be highly nonlinear task since many different parameters are involved in the model. To avoid the analytical model in this article soft computing approach was used since the soft computing approach does not require internal knowledge of the vibration model. For the soft computing model there is enough to collect the input output data pairs through experimental measurement procedure. Based on the input/output data pairs the model will be created. The approach should rank the influence of the measuring positions vibration on the pumping aggregate. Finally three different soft computing methods were compared and results were reported

SMAD4 Gene Analysis in Patients with Early Onset Colorectal Cancer: A Pilot Study

In colorectal cancer (CRC), inactivation of SMAD4 occurs early in the disease development and SMAD4 represents one of key driver genes in progression and metastasis. Loss of SMAD4 protein expression is a relatively common feature of sporadic colorectal cancers, and it was observed to be even more frequent in tumors of patients with early onset disease and also more frequent in microsatellite stable tumors. Pathogenic variants in the SMAD4 gene are usually missense or nonsense mutations, and they are more frequent in the C-terminal domain. The aim of this study was to perform genetic analysis of SMAD4 C-terminal domain in colorectal cancer patients with early onset disease and microsatellite stable tumors. This pilot study was conducted with a purpose of investigating if such genetic screening strategy would be useful for diagnostic purposes in this specific subgroup of CRC patients. The study was conducted in a selected set of DNA samples extracted from the tumors of CRC patients who had less than 50 years at the time of diagnosis. Genetic analysis of C-terminal domain has encompassed analysis of exons 9, 10, 11 and 12 of the SMAD4 gene by PCR and direct DNA sequencing. Among the twenty analyzed tumor DNAs, one sample was found to harbor a SMAD4 variant: NC_000018.9:g.48591918C gt T; (NM005359.5: c.1081C gt T; Arg361Cys). The variant was discovered in exon 9, affecting the codon 361, which represents a mutational hot spot within the SMAD4 gene. This variant was discovered in homozygous state in the tumor of a 47 yr old female with T3 stage carcinoma of the right colon. Considering the incidence and functional consequences of SMAD4 exon 9 variants, the screening of this region could be a useful low cost strategy for the genetic analysis of colorectal tumors from patients with early onset disease, as well as for susceptibility testing

Upotreba nenutritivnih adsorptivnih materija u cilju preveniranja i otklanjanja štetnih efekata mikotoksina

This review article is treating the use of several non-nutritive adsorbents in vitro and their in vivo application as possible solution it treating mycotoxicosis. Adsorbents reviewed were: zeolites, bentonites, HSCAS, various clays and active charcoal. It is shown that with the addition of those non-nutritive adsorbents the effects of mycotoxins, that may be present in significant amounts in animal feeds, could be neutralized to some degree. It must be stressed that there is no single substance that is equally effective in adsorption of all mycotoxins, also that no substance is able to completely eliminate negative mycotoxin effects. .Ovaj pregledni rad razmatra upotrebu nekoliko različitih adsorbenata in vitro i njihovu primenu in vivo kao mogući pristup u rešavanju mikotoksikoza. Prikazani adsorbenti su bili: zeoliti, bentoniti i HSCAS. U raduje prikazano da se dodavanjem navedenih nenutritivnih adsorptivnih materija sa većim ili manjim uspehom može neutralizovati dejstvo mikotoksina, koji mogu da budu značajno zastupljeni u stočnoj hrani. Treba istaći da ne postoji materija podjednako efikasna u adsorbciji svih mikotoksina niti takva koja bi u potpunosti eliminisala negativan uticaj mikotoksina