Nursing care of a patient after total hip replacement

Ústav ošetřovatelstvíDepartment of nursingThird Faculty of Medicine3. lékařská fakult

Regulace a poruchy savčí cytochrom c oxidázy.

Cytochrom c oxidáza (COX) je koncovým enzymovým komplexem dýchacího řetězce a vyskytuje se ve vnitřní mitochondriální membráně jako monomer, dimer a ve formě respiračních superkomplexů. Asemblační proces COX je komplikovaný, vysoce regulovaný a závisí na mnoha pomocných proteinech. Mutace COX podjednotek, kódovaných mitochondriální nebo jadernou DNA, nebo mutace v genech pro COX asemblační proteiny jsou častopu příčinou závažných mitochondriálních onemocnění. SURF1 protein je zapojen do počátečních fází tvorby COX, ale jeho přesná funkce není objasněna. Mutace lidského SURF1 genu vedou k těžkému defektu COX a fatálnímu neurodegenerativnímu onemocnění, Leigh syndromu. Knockout SURF1 genu u myši způsobuje také izolovaný COX defekt, ale méně výrazný a bez postižení CNS. Cílem práce byla detailní analýza narušené COX biogeneze vyvolané mutací nebo knockoutem SURF1 genu, od tvorby COX monomeru a vestavění COX do superkomplexů až po ovlivnění ostatních OXPHOS komplexů izolovaným defektem COX. Mutace SURF1 genu ve fibroblastech pacientů vedly k výrazné akumulaci asemblačního intermediátu COX a defektu tvorby funkčního COX monomeru, který se preferenčně vázal do I-III2-IV1 superkomplexu. COX deficience následně vedla ke zvýšení obsahu OXPHOS komplexů I, III a V. U SURF1-/- myší byl COX defekt výrazně...Cytochrome c oxidase (COX) represents the terminal enzyme complex of respiratory chain metabolic pathway and it occurs as monomer, dimer or as a part of respiratory supercomplexes in the inner mitochondrial membrane. COX assembly process is complicated, highly regulated and depends on many ancillary proteins. Mutations in COX subunits, which are encoded by mitochondrial and nuclear DNA, or in genes encoding its assembly proteins are frequent cause of very severe mitochondrial disorders. SURF1 assembly protein participates in the first steps of COX assembly, but its exact function is not yet clarified. In humans, mutations of SURF1 gene lead to severe COX defect and fatal neurodegenerative disorder, Leigh syndrome. Knockout of SURF1 gene in mouse causes isolated COX defect as well, but less pronounced and without involvement of CNS. The aim of the thesis was detailed analysis of disturbed COX biogenesis in a condition of SURF1 gene mutations or SURF1 gene knockout, from assembly of COX monomer to interaction of COX into supercomplexes, and to the impact of isolated COX defect on other OXPHOS complexes. Mutations of SURF1 gene in patient's fibroblasts led to marked accumulation of COX assembly intermediates and to a defect in formation of functional COX monomer, which was preferentially built into an...First Faculty of Medicine1. lékařská fakult

Financial Statement as a Source of Information for Multicriteria Analysis of State Gymnasiums in the Frýdek-Místek District

Bakalářská práce se zabývá multikriteriálním zhodnocením vybraných státních gymnázií v okrese Frýdek-Místek. Cílem je zhodnocení hospodaření na základě údajů z vybraných účetních výkazů v období 2016-2021. Cíle je dosaženo pomocí multikriteriální analýzy metodou váženého součtu s použitím vah stejné důležitosti pro všechny kritéria. Pro každou analýzu bylo vybráno právě 10 kritérií. Nejlépe hodnoceným gymnáziem za celé období na pomoci analýzy na základě dat z rozvahy se stalo gymnázium Cihelné. Analýzou za základě dat z výkazu zisků a ztrát bylo vyhodnoceno jako nejlepší subjekt gymnázium Petra Bezruče.The Bachelor's thesis deals with the multicriteria evaluation of selected state gymnasiums in the Frýdek-Místek district. The aim is evaluate the economy based on data from selected financial statements in the period 2016-2021. The aim is achieved through multicriteria analysis using the weighted sum approach method. 10 criteria were chosen for each analysis. Cihelní gymnasium became the best-rated gymnasium by multicriteria analysis based on data from balance sheet. Gymnasium Petra Bezruče became the best-rated gymnasium by multicriteria analysis bysed on data from profit and loss account.117 - Katedra účetnictví a danívýborn

Subjective perception of life quality in Písek's retirement community

The topic of this bachelor thesis is a subjective perception of life quality of senior citizens in Písek's retirement community. When the age is higher, a quality life has its importance. In most of the time, the quality of life has a link with preparedness for aging. An important factor is a level of an autonomy and vastness of changes, which connect with the aging. Health condition is also important, because it plays major role in limiting the senior citizens. Theoretical part is focused on factors, which have impact on life quality. This thesis mention terms like age, aging, types of age and changes happening during aging. The changes can have physical, mental or social background. The aim of the practical part of this thesis is to find out how the senior citizens in Písek's retirement community perceive a quality of life. The research was made via SEIQoL method. Complementary method is represented by semi-structured interview. Three men and five women, all of them in different age and living in Písek's retirement community, were chosen for the interview. Each of the respondents live in Písek's retirement community for a different period of time. The research reveals a fact, that most of the senior citizens have same or similar life goals. They are satisfied with the goals. The quality of life index was in six cases higher than 70 %. Partial aim of the thesis was to find out reasons why and in which cases the senior citizens do not meet high quality life. Eventually I found out, that the reasons for not fulfilling the life goals of senior citizens are different in each case so the question cannot be conclusively answered

Investigation of knock-out mice with the deficiency of cytochrome c oxidase using advenced electrophoretical techniques

Department of BiochemistryKatedra biochemiePřírodovědecká fakultaFaculty of Scienc

Regulation and Disorders of Mammalian Cytochrome c Oxidase

Cytochrome c oxidase (COX) represents the terminal enzyme complex of respiratory chain metabolic pathway and it occurs as monomer, dimer or as a part of respiratory supercomplexes in the inner mitochondrial membrane. COX assembly process is complicated, highly regulated and depends on many ancillary proteins. Mutations in COX subunits, which are encoded by mitochondrial and nuclear DNA, or in genes encoding its assembly proteins are frequent cause of very severe mitochondrial disorders. SURF1 assembly protein participates in the first steps of COX assembly, but its exact function is not yet clarified. In humans, mutations of SURF1 gene lead to severe COX defect and fatal neurodegenerative disorder, Leigh syndrome. Knockout of SURF1 gene in mouse causes isolated COX defect as well, but less pronounced and without involvement of CNS. The aim of the thesis was detailed analysis of disturbed COX biogenesis in a condition of SURF1 gene mutations or SURF1 gene knockout, from assembly of COX monomer to interaction of COX into supercomplexes, and to the impact of isolated COX defect on other OXPHOS complexes. Mutations of SURF1 gene in patient's fibroblasts led to marked accumulation of COX assembly intermediates and to a defect in formation of functional COX monomer, which was preferentially built into an..

Nursing care of a patient after total hip replacement

Ústav ošetřovatelstvíDepartment of nursingThird Faculty of Medicine3. lékařská fakult

Regulation and Disorders of Mammalian Cytochrome c Oxidase

Cytochrome c oxidase (COX) represents the terminal enzyme complex of respiratory chain metabolic pathway and it occurs as monomer, dimer or as a part of respiratory supercomplexes in the inner mitochondrial membrane. COX assembly process is complicated, highly regulated and depends on many ancillary proteins. Mutations in COX subunits, which are encoded by mitochondrial and nuclear DNA, or in genes encoding its assembly proteins are frequent cause of very severe mitochondrial disorders. SURF1 assembly protein participates in the first steps of COX assembly, but its exact function is not yet clarified. In humans, mutations of SURF1 gene lead to severe COX defect and fatal neurodegenerative disorder, Leigh syndrome. Knockout of SURF1 gene in mouse causes isolated COX defect as well, but less pronounced and without involvement of CNS. The aim of the thesis was detailed analysis of disturbed COX biogenesis in a condition of SURF1 gene mutations or SURF1 gene knockout, from assembly of COX monomer to interaction of COX into supercomplexes, and to the impact of isolated COX defect on other OXPHOS complexes. Mutations of SURF1 gene in patient's fibroblasts led to marked accumulation of COX assembly intermediates and to a defect in formation of functional COX monomer, which was preferentially built into an..

Characterisation of respiratory supercomplexes in various types of mammalian mitochondria

Department of BiochemistryKatedra biochemiePřírodovědecká fakultaFaculty of Scienc