Dengue Virus Infection of Aedes aegypti Requires a Putative Cysteine Rich Venom Protein

Citation: Londono-Renteria, B., Troupin, A., Conway, M. J., Vesely, D., Ledizet, M., Roundy, C. M., . . . Colpitts, T. M. (2015). Dengue Virus Infection of Aedes aegypti Requires a Putative Cysteine Rich Venom Protein. Plos Pathogens, 11(10), 23. doi:10.1371/journal.ppat.1005202Dengue virus (DENV) is a mosquito-borne flavivirus that causes serious human disease and mortality worldwide. There is no specific antiviral therapy or vaccine for DENV infection. Alterations in gene expression during DENV infection of the mosquito and the impact of these changes on virus infection are important events to investigate in hopes of creating new treatments and vaccines. We previously identified 203 genes that were >= 5-fold differentially upregulated during flavivirus infection of the mosquito. Here, we examined the impact of silencing 100 of the most highly upregulated gene targets on DENV infection in its mosquito vector. We identified 20 genes that reduced DENV infection by at least 60% when silenced. We focused on one gene, a putative cysteine rich venom protein (SeqID AAEL000379; CRVP379), whose silencing significantly reduced DENV infection in Aedes aegypti cells. Here, we examine the requirement for CRVP379 during DENV infection of the mosquito and investigate the mechanisms surrounding this phenomenon. We also show that blocking CRVP379 protein with either RNAi or specific antisera inhibits DENV infection in Aedes aegypti. This work identifies a novel mosquito gene target for controlling DENV infection in mosquitoes that may also be used to develop broad preventative and therapeutic measures for multiple flaviviruses

Office Lighting - current state and problems

Office lighting is a rapidly developing field in which new technologies are constantly introduced. In this work a review of the state of office lighting technology has been made, both generally and with a case example. The HUT Valotalo was used as the case. A survey was made to find out the problems and development needs of office lighting. This survey included all actors active in the various phases of the life cycle of an office lighting installation. The survey did not reveal anyone significant problem; the problems are associated more with methods than the devices.Toimistovalaistus kehittyy nopeasti ja uusia teknologioita kehitetään markkinoille jatkuvasti. Työssä on luotu katsaus toimistovalaistusteknologian tilaan vuonna 2001. sekä yleensä että esimerkkikohteen kautta. Esimerkkikohteena käytettiin TKK:n Valotaloa. Tämän lisäksi työssä tehtiin kyselytutkimus toimistovalaistustekniikassa ilmenevien ongelmien ja kehitystarpeitten kartoittamiseksi. Tutkimus käsitti kaikki valaistusasennuksen elinkaareen liittyvät tahot. Valaistusteknologiassa ei ole yhtä merkittävää ongelmakohtaa, ja ongelmat ilmenevat yleensä enemmän toimintatavoissa kuin itse laitteissa