5 research outputs found
Investigação dos efeitos dos antivirais aciclovir e ganciclovir sobre o desenvolvimento reprodutivo de ratos machos expostos in utero
Resum
Investigação dos efeitos dos antivirais aciclovir e ganciclovir sobre o desenvolvimento reprodutivo de ratos machos expostos in utero
Resum
Influence of three different histological methods on the morphology and morphometrical data in human testis
Coagulant fixatives and paraffin embedding
were widely used in the past for histomorphometrical
evaluations of the human testis under physiological and
pathological conditions. However, new methods are
applied nowadays using better combinations of fixatives
and plastic resins as embedding media, improving cell
and tissue structural preservation. In an attempt to
compare old and new data, the present study evaluated
histomorphometrical data obtained from human testis
after three different histological processing methods:
Bouin/paraplast, glutaraldehyde/glycol methacrylate and
glutaraldehyde/araldite. The morphometrical parameters
were not affected by glutaraldehyde fixation after both
resin embedding (methacrylate or araldite). On the other
hand, Bouin/paraplast embedding lead to tissue
shrinkage, which could give rise to misinterpretations on
the measurements performed. Since some germ and
somatic cells recognition do not depend upon high
resolution techniques, counting of such cell types could
be performed even using routine Bouin/paraplast
protocols. Thus, the morphometrical analyses relying on
cell recognition were not affected by the methods here
applied, however, when metric measurements were
applied, the obtained results could not be promptly
compared. On the other hand, if the study requires
confident spermatogonial identification for kinetics
evaluation, glutaraldehyde/araldite processing is highly
recommended
Exploratory comparisons between different anti-mitotics in clinically-used drug combination in triple negative breast cancer
Triple-negative breast cancer (TNBC) constitutes a very aggressive type of breast cancer with few options of cytotoxic chemotherapy available for them. A chemotherapy regimen comprising of doxorubicin hydrochloride and cyclophosphamide, followed by paclitaxel, known as AC-T, is approved for usage as an adjuvant treatment for this type of breast cancer. In this study we aimed to elucidate the role of KIF11 in TNBC progression throughout its inhibition by two synthetic small molecules containing the DHPM core (dihydropyrimidin-2(1H)-ones or -thiones), with the hypothesis that these inhibitors could be an interesting option of antimitotic drug used alone or as adjuvant therapy in association with AC. For this purpose, we evaluated the efficacy of DHPMs used as monotherapy or in combination with doxorubicin and cyclophosphamide, in Balbc-nude mice bearing breast cancer induced by MDA-MB-231, having AC-T as positive control. Our data provide extensive evidence to demonstrate that KIF11 inhibitors showed pronounced antitumor activity, acting in key points of tumorigenesis and cancer progression in in vivo xenograft model of triple negative breast cancer, like down-regulation of KIF11 and ALDH1-A1. Moreover, they didn’t show the classic peripheral neuropathy characterized by impaired mobility, as it is common with paclitaxel use. These results suggest that the use of a MAP inhibitor in breast cancer regimen treatment could be a promising strategy to keep antitumoral activity reducing the side effects