Iron Age Settlement and Cemetery at Sejlflod in Himmerland, North Jutland

Iron Age Settlement and Cemetery at Sejlflod in Himmerland, North Jutlan

The chronology and structure of the Sejlflod cemetery, Northern Jutland, Denmark

The Sejlflod cemetery in Northern Jutland, containing almost 300 graves from the Late Roman and Early Germanic Iron Age, occupies a central position in a North European perspective. This arises in particular from the fact that the graves are inhumation burials furnished with a relative abundance of grave goods and that the cemetery represents the entire adult population of a village through time. An understanding of the Sejlflod cemetery is important for investigations of other similar cemeteries and burial grounds, for studies of a range of period-defining artefacts and for analyses of the social circumstances of the time. It is, however, heavily dependent on knowledge of the cemetery’s chronological structure. On the basis of the pottery, it has proved possible to divide the cemetery up into four chronological phases. This division is supported by stylistic and chronological analyses of the fibulas and a few other artefact types from the graves. Surprisingly, the chronological analysis does not reveal a horizontal stratigraphical development. On the contrary, it provides a basis for a new interpretation of the cemetery as a progressive fusion of independent family grave clusters

Mapping the interaction of Snf1 with TORC1 in Saccharomyces cerevisiae

Nutrient sensing and coordination of metabolic pathways are crucial functions for living cells. A combined analysis of the yeast transcriptome, phosphoproteome and metabolome is used to investigate the interactions between the Snf1 and TORC1 pathways under nutrient-limited conditions

Analysis of normal levels of free glycosaminoglycans in urine and plasma in adults

Plasma and urine glycosaminoglycans (GAGs) are long, linear sulfated polysaccharides that have been proposed as potential noninvasive biomarkers for several diseases. However, owing to the analytical complexity associated with the measurement of GAG concentration and disaccharide composition (the so-called GAGome), a reference study of the normal healthy GAGome is currently missing. Here, we prospectively enrolled 308 healthy adults and analyzed their free GAGomes in urine and plasma using a standardized ultra-high-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry method together with comprehensive demographic and blood chemistry biomarker data. Of 25 blood chemistry biomarkers, we mainly observed weak correlations between the free GAGome and creatinine in urine and hemoglobin or erythrocyte counts in plasma. We found a higher free GAGome concentration - but not a more diverse composition - in males. Partitioned by gender, we also established reference intervals for all detectable free GAGome features in urine and plasma. Finally, we carried out a transference analysis in healthy individuals from two distinct geographical sites, including data from the Lifelines Cohort Study, which validated the reference intervals in urine. Our study is the first large-scale determination of normal free GAGomes reference intervals in plasma and urine and represents a critical resource for future physiology and biomarker research