Turning Meiosis into Mitosis

The mutation of as few as three genes in a sexual plant transforms meiosis into mitosis and results in diploid gametes that are genetically identical to the mother plant. This phenotype resembles apomeiosis, which is a major step in apomixis

Mutations in AtPS1 (Arabidopsis thaliana Parallel Spindle 1) Lead to the Production of Diploid Pollen Grains

Polyploidy has had a considerable impact on the evolution of many eukaryotes, especially angiosperms. Indeed, most—if not all—angiosperms have experienced at least one round of polyploidy during the course of their evolution, and many important crop plants are current polyploids. The occurrence of 2n gametes (diplogametes) in diploid populations is widely recognised as the major source of polyploid formation. However, limited information is available on the genetic control of diplogamete production. Here, we describe the isolation and characterisation of the first gene, AtPS1 (Arabidopsis thaliana Parallel Spindle 1), implicated in the formation of a high frequency of diplogametes in plants. Atps1 mutants produce diploid male spores, diploid pollen grains, and spontaneous triploid plants in the next generation. Female meiosis is not affected in the mutant. We demonstrated that abnormal spindle orientation at male meiosis II leads to diplogamete formation. Most of the parent's heterozygosity is therefore conserved in the Atps1 diploid gametes, which is a key issue for plant breeding. The AtPS1 protein is conserved throughout the plant kingdom and carries domains suggestive of a regulatory function. The isolation of a gene involved in diplogamete production opens the way for new strategies in plant breeding programmes and progress in evolutionary studies

The role of Interferometric Synthetic Aperture Radar in Detecting, Mapping, Monitoring, and Modelling the Volcanic Activity of Piton de la Fournaise, La Réunion: A Review

Synthetic Aperture Radar (SAR) remote sensing plays a significant role in volcano monitoring despite the measurements’ non real-time nature. The technique’s capability of imaging the spatial extent of ground motion has especially helped to shed light on the location, shape, and dynamics of subsurface magmatic storage and transport as well as the overall state of activity of volcanoes worldwide. A variety of different deformation phenomena are observed at exceptionally active and frequently erupting volcanoes, like Piton de la Fournaise on La Réunion Island. Those offer a powerful means of investigating related geophysical source processes and offer new insights into an active volcano’s edifice architecture, stability, and eruptive behavior. Since 1998, Interferometric Synthetic Aperture Radar (InSAR) has been playing an increasingly important role in developing our present understanding of the Piton de la Fournaise volcanic system. We here collect the most significant scientific results, identify limitations, and summarize the lessons learned from exploring the rich Piton de la Fournaise SAR data archive over the past ~20 years. For instance, the technique has delivered first evidence of the previously long suspected mobility of the volcano’s unsupported eastern flank, and it is especially useful for detecting displacements related to eruptions that occur far away from the central cone, where Global Navigation Satellite System (GNSS) stations are sparse. However, superimposed deformation processes, dense vegetation along the volcano’s lower eastern flank, and turbulent atmospheric phase contributions make Piton de la Fournaise a challenging target for applying InSAR. Multitemporal InSAR approaches that have the potential to overcome some of these limitations suffer from frequent eruptions that cause the replacement of scatterers. With increasing data acquisition rates, multisensor complementarity, and advanced processing techniques that resourcefully handle large data repositories, InSAR is progressively evolving into a near-real-time, complementary, operational volcano monitoring tool. We therefore emphasize the importance of InSAR at highly active and well-monitored volcanoes such as Mount Etna, Italy, Kīlauea Volcano, Hawai’i, and Piton de la Fournaise, La Réunion

pur4 Mutations Are Lethal to the Male, But Not the Female, Gametophyte and Affect Sporophyte Development in Arabidopsis[C][W]

Purine metabolism is crucial in living cells and involves three complex pathways in plants: the de novo synthesis, the salvage, and the degradation pathways. The relative importance of each pathway in plant development and reproduction, however, is still unclear. We identified two T-DNA insertions in the Arabidopsis (Arabidopsis thaliana) PUR4 gene (At1g74260) that encodes formylglycinamidine ribonucleotide synthase (EC 6.3.5.3), the fourth enzyme in the de novo purine biosynthesis pathway. The mutated alleles were never transmitted through the pollen of heterozygous plants but could be inherited through the female gametophyte, indicating that de novo purine synthesis is specifically necessary for pollen development. Because the pur4 mutations were lethal to the male gametophyte, homozygous pur4 plants could not be obtained. However, the reproductive phenotype of hetererozygous plants carrying the pur4-2 mutated allele was more severe than that carrying the pur4-1 mutated allele, and pur4-2/+ plants showed slightly delayed early development. We showed that the pur4-2 allele produces an antisense transcript and that the amount of PUR4 mRNA is reduced in these plants. Transient expression of a translational fusion with the green fluorescent protein in Arabidopsis plantlets showed that the formylglycinamidine ribonucleotide synthase protein is dually targeted to chloroplast and mitochondria, suggesting that at least some steps of the de novo purine biosynthesis pathway can take place in both organelles in Arabidopsis, a dual location previously thought to be a peculiarity of ureide-forming tropical legumes

From Arabidopsis thaliana to Brassica napus : development of amplified consensus genetic markers (ACGM)for construction of a gene map

International audienc

MCM8 is required for a pathway of meiotic double-strand break repair independent of DMC1 in Arabidopsis thaliana

Mini-chromosome maintenance (MCM) 2-9 proteins are related helicases. The first six, MCM2-7, are essential for DNA replication in all eukaryotes. In contrast, MCM8 is not always conserved in eukaryotes but is present in Arabidopsis thaliana. MCM8 is required for 95% of meiotic crossovers (COs) in Drosophila and is essential for meiosis completion in mouse, prompting us to study this gene in Arabidopsis meiosis. Three allelic Atmcm8 mutants showed a limited level of chromosome fragmentation at meiosis. This defect was dependent on programmed meiotic double-strand break (DSB) formation, revealing a role for AtMCM8 in meiotic DSB repair. In contrast, CO formation was not affected, as shown both genetically and cytologically. The Atmcm8 DSB repair defect was greatly amplified in the absence of the DMC1 recombinase or in mutants affected in DMC1 dynamics (sds, asy1). The Atmcm8 fragmentation defect was also amplified in plants heterozygous for a mutation in either recombinase, DMC1 or RAD51. Finally, in the context of absence of homologous chromosomes (i.e. haploid), mutation of AtMCM8 also provoked a low level of chromosome fragmentation. This fragmentation was amplified by the absence of DMC1 showing that both MCM8 and DMC1 can promote repair on the sister chromatid in Arabidopsis haploids. Altogether, this establishes a role for AtMCM8 in meiotic DSB repair, in parallel to DMC1. We propose that MCM8 is involved with RAD51 in a backup pathway that repairs meiotic DSB without giving CO when the major pathway, which relies on DMC1, fails

AAA-ATPase FIDGETIN-LIKE 1 and helicase FANCM antagonize meiotic crossovers by distinct mechanisms

Meiotic crossovers (COs) generate genetic diversity and are critical for the correct completion of meiosis in most species. Their occurrence is tightly constrained but the mechanisms underlying this limitation remain poorly understood. Here we identified the conserved AAA-ATPase FIDGETIN-LIKE-1 (FIGL1) as a negative regulator of meiotic CO formation. We show that Arabidopsis FIGL1 limits CO formation genome-wide, that FIGL1 controls dynamics of the two conserved recombinases DMC1 and RAD51 and that FIGL1 hinders the interaction between homologous chromosomes, suggesting that FIGL1 counteracts DMC1/RAD51-mediated inter-homologue strand invasion to limit CO formation. Further, depleting both FIGL1 and the previously identified anti-CO helicase FANCM synergistically increases crossover frequency. Additionally, we showed that the effect of mutating FANCM on recombination is much lower in F1 hybrids contrasting from the phenotype of inbred lines, while figl1 mutation equally increases crossovers in both contexts. This shows that the modes of action of FIGL1 and FANCM are differently affected by genomic contexts. We propose that FIGL1 and FANCM represent two successive barriers to CO formation, one limiting strand invasion, the other disassembling D-loops to promote SDSA, which when both lifted, leads to a large increase of crossovers, without impairing meiotic progression

<i>osd1</i> mutants skip meiosis II.

<p>(A and B) Male meiotic products stained with toluidine blue. (A) A wild-type tetrad. (B) A dyad in the <i>osd1-1</i> mutant. (C–D) Male meiosis in <i>osd1</i> is indistinguishable from wild type until telophase I (compare to <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1000124#pbio-1000124-g004" target="_blank">figure 4</a>), but no figures characteristic of a second division were observed. (C) Pachytene. (D) Diakinesis. (E) Metaphase I. (F) Anaphase I. (G) Telophase I. Two nuclei separated by a band of mitochondria are observed. (H) Metaphase I of female meiosis in <i>osd1</i>.</p