Induction of OCT2 contributes to regulate the gene expression program in human neutrophils activated via TLR8

The transcription factors (TFs) that regulate inducible genes in activated neutrophils are not yet completely characterized. Herein, we show that the genomic distribution of the histone modification H3K27Ac, as well as PU.1 and C/EBP beta, two myeloid-lineage-determining TFs (LDTFs), significantly changes in human neutrophils treated with R848, a ligand of Toll-like receptor 8 (TLR8). Interestingly, differentially acetylated and LDTF-marked regions reveal an over-representation of OCT-binding motifs that are selectively bound by OCT2/POU2F2. Analysis of OCT2 genomic distribution in primary neutrophils and of OCT2-depletion in HL-60-differentiated neutrophils proves the requirement for OCT2 in contributing to promote, along with nuclear factor kappa B (NF-kappa B) and activator protein 1 (AP-1), the TLR8-induced gene expression program in neutrophils. Altogether, our data demonstrate that neutrophils, upon activation via TLR8, profoundly reprogram their chromatin status, ultimately displaying cell-specific, prolonged transcriptome changes. Data also show an unexpected role for OCT2 in amplifying the transcriptional response to TLR8-mediated activation

Abuses and Distortions in Emotional Processes as Risk Factors for Parricide

Parricide is a rare case of murder having a strong emotional impact on public opinion, especially when it is heinous and perpetrated by minors. Based on the analysis of the judicial files archived at the Minor Penal Institute "N. Fornelli" of Bari, the authors will report three impressive cases perpetrated by minors, by focusing on their emotional features. Differences between the crimes of parricide committed by adults and minors will be highlighted, by analyzing the peculiar relations between the problems underlying parricide and the role played by abuses and maltreatments experienced by minors perpetrating such crime. In particular, attention will be paid to the emotional processes aroused in minor parricide offenders

Chromatin remodelling and autocrine TNFα are required for optimal interleukin-6 expression in activated human neutrophils

How IL-6 expression is regulated in human neutrophils has remained unclear. Here the authors show, using highly purified neutrophils, that TLR8 or TLR4 signalling activates latent enhancers and cooperates with autocrine TNFα to induce IL-6 transcription

Riforme amministrative e viabilità nel Regno di Napoli durante il periodo francese

Durante il decennio francese la viabilità principale del Regno di Napoli non conseguì vistosi successi in termini quantitativi. Finanziamenti ed estensione della rete rimasero pressoché invariati rispetto al precedente periodo borbonico. Qualche successo si potè rilevare nella costruzione dei ponti e nelle manutenzioni, che consentirono l'utilizzazione delle infrastrutture anche nel periodo invernale. La nuova macchina amministrativa dello stato, invece, consentì una migliore conoscenza delle necessità delle province e della loro effettiva capacità contributiva, mentre il Corpo degli ingegneri di ponti e strade, che si inserì nel nuovo contesto amministrativo, grazie alle sue articolazioni dipartimentali, fu in grado di seguire meglio lo sviluppo dei lavori e di elaborare piani più realistici che, però, rimasero allo stato di progetti per la breve durata dei regni napoleonici.Ostuni Nicola. Riforme amministrative e viabilità nel Regno di Napoli durante il periodo francese. In: Villes et territoire pendant la période napoléonienne (France et Italie). Actes du colloque de Rome (3-5 mai 1984) Rome : École Française de Rome, 1987. pp. 161-181. (Publications de l'École française de Rome, 96

Epigenetic regulation of neutrophil development and function

In addition to performing well-defined effector functions, neutrophils are now recognized as versatile and sophisticated cells with critical immunoregulatory roles. These include the release of a variety of proinflammatory or immunosuppressive cytokines, as well as the expression of genes with regulatory functions. Neutrophils share broad transcriptional features with monocytes, in keeping with the close developmental relation between the two cell types. However, neutrophil-specific gene expression patterns conferring cell type-specific responses to bacterial, viral or fungal components have been identified. Accumulating evidence suggest that these differences reflect the peculiar epigenomic and regulatory landscapes of neutrophils and monocytes, in turn controlled by the specific lineage-determining transcription factors shaping their identity. In this review, we will describe current knowledge on how neutrophil identity and function are controlled at the molecular level, focusing on transcriptional and chromatin regulation of neutrophil development and activation in response to inflammatory stimuli