Non-Local Finite-Size Effects in the Dimer Model

We study the finite-size corrections of the dimer model on $\infty \times N$ square lattice with two different boundary conditions: free and periodic. We find that the finite-size corrections depend in a crucial way on the parity of $N$, and show that, because of certain non-local features present in the model, a change of parity of $N$ induces a change of boundary condition. Taking a careful account of this, these unusual finite-size behaviours can be fully explained in the framework of the $c=-2$ logarithmic conformal field theory.Comment: This is a contribution to the Proc. of the O'Raifeartaigh Symposium on Non-Perturbative and Symmetry Methods in Field Theory (June 2006, Budapest, Hungary), published in SIGMA (Symmetry, Integrability and Geometry: Methods and Applications) at http://www.emis.de/journals/SIGMA

New bounds on the neutrino magnetic moment from the plasma induced neutrino chirality flip in a supernova

The neutrino chirality-flip process under the conditions of the supernova core is investigated in detail with the plasma polarization effects in the photon propagator taken into account, in a more consistent way than in earlier publications. It is shown in part that the contribution of the proton fraction of plasma is essential. New upper bounds on the neutrino magnetic moment are obtained: mu_nu < (0.5 - 1.1) 10^{-12} mu_B from the limit on the supernova core luminosity for nu_R emission, and mu_nu < (0.4 - 0.6) 10^{-12} mu_B from the limit on the averaged time of the neutrino spin-flip. The best upper bound on the neutrino magnetic moment from SN1987A is improved by the factor of 3 to 7.Comment: 19 pages, LaTeX, 7 EPS figures, submitted to Journal of Cosmology and Astroparticle Physic

Scaling Relations of Dwarf Galaxies without Supernova-Driven Winds

Nearby dwarf galaxies exhibit tight correlations between their global stellar and dynamical properties, such as circular velocity, mass-to-light ratio, stellar mass, surface brightness, and metallicity. Such correlations have often been attributed to gas or metal-rich outflows driven by supernova energy feedback to the interstellar medium. We use high-resolution cosmological simulations of high-redshift galaxies with and without energy feedback, as well as analytic modeling, to investigate whether the observed correlations can arise without supernova-driven outflows. We find that the simulated dwarf galaxies exhibit correlations similar to those observed as early as z~10, regardless of whether supernova feedback is included. We also show that the correlations can be well reproduced by our analytic model that accounts for realistic gas inflow but assumes no outflows, and star formation rate obeying the Kennicutt-Schmidt law with a critical density threshold. We argue that correlations in simulated galaxies arise due to the increasingly inefficient conversion of gas into stars in low-mass dwarf galaxies rather than supernova-driven outflows. We also show that the decrease of the observed effective yield in low-mass objects, often used as an indicator of gas and metal outflows, can be reasonably reproduced in our simulations without outflows. We show that this trend can arise if a significant fraction of metals in small galaxies is spread to the outer regions of the halo outside the stellar extent via mixing. In this case the effective yield can be significantly underestimated if only metals within the stellar radius are taken into account. Measurements of gas metallicity in the outskirts of gaseous disks of dwarfs would thus provide a key test of such explanation.Comment: accepted for publication in ApJ, 19 pages, 12 figures, uses emulateapj

Composition and properties of water-soluble preparation from salmon liver

Amino acid composition and antioxidant activity are investigated for dried liver of chum salmon Oncorhynchus keta , for water-soluble preparation from the dried liver, and for 1-10 kDa fraction of the water-soluble preparation. The water-soluble preparation contains more than 60 % peptides of molecular weight 1-10 kDa, about 18 % peptides of > 10 kDa, and about 20 % low molecular peptides of molecular weight < 1 kDa. The content of combined amino acids is the highest in the water-soluble preparation (672.5 mg/g), it is lower in its 1-10 kDa fraction (544.4 mg/g) and the lowest in the dried liver (333.3 mg/g). On the contrary, the content of free ninhydrin-positive compounds is the highest in the liver (91.64 mg/g) and the lowest in the water-soluble preparation (61.36 mg/g). Relative to the level of carnosine antioxidant activity, the antioxidant activity of the dried liver is higher in 5.2 times, the activity of the water-soluble preparation is higher in 4.2 times, and the activity of the 1-10 kDa fraction is higher in 6.3 times. Three isoforms of such biologically active peptide as hepcidin are found in the 1-10 kDa fraction, their molecular weights are 2841.54, 2913.54 and 3025.63 Da

Loss of Nmp4 optimizes osteogenic metabolism and secretion to enhance bone quality

A goal of osteoporosis therapy is to restore lost bone with structurally sound tissue. Mice lacking the transcription factor Nuclear Matrix Protein 4 (Nmp4, Zfp384, Ciz, ZNF384) respond to several classes of osteoporosis drugs with enhanced bone formation compared to wild type (WT) animals. Nmp4-/- mesenchymal stem/progenitor cells (MSPCs) exhibit an accelerated and enhanced mineralization during osteoblast differentiation. To address the mechanisms underlying this hyper-anabolic phenotype, we carried out RNA-sequencing and molecular and cellular analyses of WT and Nmp4-/- MSPCs during osteogenesis to define pathways and mechanisms associated with elevated matrix production. We determined that Nmp4 has a broad impact on the transcriptome during osteogenic differentiation, contributing to the expression of over 5,000 genes. Phenotypic anchoring of transcriptional data was performed for the hypothesis-testing arm through analysis of cell metabolism, protein synthesis and secretion, and bone material properties. Mechanistic studies confirmed that Nmp4-/- MSPCs exhibited an enhanced capacity for glycolytic conversion- a key step in bone anabolism. Nmp4-/- cells showed elevated collagen translation and secretion. Expression of matrix genes that contribute to bone material-level mechanical properties were elevated in Nmp4-/- cells, an observation that was supported by biomechanical testing of bone samples from Nmp4-/- and WT mice. We conclude that loss of Nmp4 increases the magnitude of glycolysis upon the metabolic switch, which fuels the conversion of the osteoblast into a super-secretor of matrix resulting in more bone with improvements in intrinsic quality

A New Constraint on the Escape Fraction in Distant Galaxies Using Gamma-ray Burst Afterglow Spectroscopy

We describe a new method to measure the escape fraction fesc of ionizing radiation from distant star-forming galaxies using the afterglow spectra of long-duration gamma-ray bursts (GRBs). Optical spectra of GRB afterglows allow us to evaluate the optical depth of the host ISM, according to the neutral hydrogen column density N(HI) observed along the sightlines toward the star-forming regions where the GRBs are found. Different from previous effort in searching for faint, transmitted Lyman continuum photons, our method is not subject to background subtraction uncertainties and does not require prior knowledge of either the spectral shape of the host galaxy population or the IGM Lya forest absorption along these GRB sightlines. Because most GRBs occur in sub-L_* galaxies, our study also offers the first constraint on fesc for distant low-mass galaxies that dominate the cosmic luminosity density. We have compiled a sample of 27 GRBs at redshift z>2 for which the underlying N(HI) in the host ISM are known. These GRBs together offer a statistical sampling of the integrated optical depth to ionizing photons along random sightlines from star-forming regions in the host galaxies, and allow us to estimate the mean escape fraction averaged over different viewing angles. We find =0.02\pm 0.02 and place a 95% c.l. upper limit <= 0.075 for these hosts. We discuss possible biases of our approach and implications of the result. Finally, we propose to extend this technique for measuring at z~0.2 using spectra of core-collapse supernovae.Comment: Five journal pages, including one figure; ApJL in pres

Crystal structure of alkyl hydroperoxidase D like protein PA0269 from Pseudomonas aeruginosa: Homology of the AhpD-like structural family

<p>Abstract</p> <p>Background</p> <p>Alkyl hydroperoxidase activity provides an important antioxidant defense for bacterial cells. The catalytic mechanism requires two peroxidases, AhpC and AhpD, where AhpD plays the role of an essential adaptor protein.</p> <p>Results</p> <p>The crystal structure of a putative AhpD from <it>Pseudomonas aeruginosa </it>has been determined at 1.9 Å. The protein has an all-helical fold with a chain topology similar to a known AhpD from <it>Mycobacterium tuberculosis </it>despite a low overall sequence identity of 9%. A conserved two α-helical motif responsible for function is present in both. However, in the <it>P. aeruginosa </it>protein, helices H3, H4 of this motif are located at the N-terminal part of the chain, while in <it>M. tuberculosis </it>AhpD, the corresponding helices H8, H9 are situated at the C-terminus. Residues 24-62 of the putative catalytic region of <it>P. aeruginosa </it>have a higher sequence identity of 33% where the functional activity is supplied by a proton relay system of five residues, Glu36, Cys48, Tyr50, Cys51, and His55, and one structural water molecule. A comparison of five other related hypothetical proteins from various species, assigned to the alkyl hydroperoxidase D-like protein family, shows they contain the same conserved structural motif and catalytic sequence Cys-X-X-Cys. We have shown that AhpD from <it>P. aeruginosa </it>exhibits a weak ability to reduce H₂O₂as tested using a ferrous oxidation-xylenol orange (FOX) assay, and this activity is blocked by thiol alkylating reagents.</p> <p>Conclusion</p> <p>Thus, this hypothetical protein was assigned to the AhpD-like protein family with peroxidase-related activity. The functional relationship of specific oligomeric structures of AhpD-like structural family is discussed.</p

Automated manufacturing of chimeric antigen receptor T cells for adoptive immunotherapy using CliniMACS prodigy

Novel cell therapies derived from human T lymphocytes are exhibiting enormous potential in early-phase clinical trials in patients with hematologic malignancies. Ex vivo modification of T cells is currently limited to a small number of centers with the required infrastructure and expertise. The process requires isolation, activation, transduction, expansion and cryopreservation steps. To simplify procedures and widen applicability for clinical therapies, automation of these procedures is being developed. The CliniMACS Prodigy (Miltenyi Biotec) has recently been adapted for lentiviral transduction of T cells and here we analyse the feasibility of a clinically compliant T-cell engineering process for the manufacture of T cells encoding chimeric antigen receptors (CAR) for CD19 (CAR19), a widely targeted antigen in B-cell malignancies. Using a closed, single-use tubing set we processed mononuclear cells from fresh or frozen leukapheresis harvests collected from healthy volunteer donors. Cells were phenotyped and subjected to automated processing and activation using TransAct, a polymeric nanomatrix activation reagent incorporating CD3/CD28-specific antibodies. Cells were then transduced and expanded in the CentriCult-Unit of the tubing set, under stabilized culture conditions with automated feeding and media exchange. The process was continuously monitored to determine kinetics of expansion, transduction efficiency and phenotype of the engineered cells in comparison with small-scale transductions run in parallel. We found that transduction efficiencies, phenotype and function of CAR19 T cells were comparable with existing procedures and overall T-cell yields sufficient for anticipated therapeutic dosing. The automation of closed-system T-cell engineering should improve dissemination of emerging immunotherapies and greatly widen applicability